High expression of COL5A2, a member of COL5 family, indicates the poor survival and facilitates cell migration in gastric cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:33739392", "kind": "science-record", "revision": 6, "content_hash": "sha256:1a3a570ffb8c88800ed291f7a98343918410d0b366944a10a047188b7abfd9ee", "created_at": "2026-11-01T15:41:29Z", "updated_at": "2026-11-01T21:24:55Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "High expression of COL5A2, a member of COL5 family, indicates the poor survival and facilitates cell migration in gastric cancer.", "doi": "10.1042/BSR20204293", "pmid": "33739392", "authors": ["Yuen Tan", "Qingchuan Chen", "Yao Xing", "Chao Zhang", "Siwei Pan", "Wen An", "Huimian Xu"], "journal": "Bioscience reports", "year": 2021, "abstract": "BACKGROUND: Gastric cancer (GC) metastasis determines the prognosis of patients, and exploring the molecular mechanism of GC metastasis is expected to provide a theoretical basis for clinical treatment. Recent studies have shown that extracellular matrix protein is closely related to GC metastasis. The present study aimed to explore the expression profile and role of COL5A2, as an extracellular matrix protein, in GC.\nMETHODS: The expression, overall survival, and progression-free survival data of COL5 family members were extracted from The Cancer Genome Atlas (TCGA) database, respectively. Weighted gene co-expression network analysis of the GSE62229 database was performed out to identify modules and associated genes.\nRESULTS: COL5A2 was selected as our research target in the TCGA database, and was also verified in the GSE62229 and GSE15459 datasets. COL5A2 was up-regulated in GC tissues by paraffin immunohistochemistry and RT-qPCR. The prognosis of patients with low COL5A2 expression was better than that of patients with high COL5A2 expression. Scratch and migration experiments showed that knockdown of COL5A2 decreased the migration ability of gastric cancer cells compared with the control group. In vivo, mice with tail vein injection COL5A2 knockdown had fewer and smaller metastatic nodules in liver. GSEA results showed that the TCGA and GSE62229 samples were significantly enriched in several well-known cancer-related pathways, such as the TGF-β, MAPK, and JAK2 signaling pathways.\nCONCLUSION: COL5A2 was most closely related to advanced GC among COL5 family members. High COL5A2 expression is associated with a poor prognosis, and may be a novel therapeutic target for GC.", "claims": [{"id": "claim-method", "statement": "The Kaplan–Meier method was used to calculate OS.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33739392", "locator": "sections[16] \"Statistical analysis\" paragraph 2", "quote": "The Kaplan–Meier method was used to calculate OS."}], "state": "complete"}, {"id": "claim-data-access", "statement": "All data in this article were available, and could find and use in the websites. K-M plotter (http://kmplot.com/), GEPIA (http://gepia.cancer-pku.cn/), and GSE62229 and GSE15459 (https://www.ncbi.nlm.nih.gov/gds).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33739392", "locator": "sections[26] \"Data Availability\" paragraph 2", "quote": "All data in this article were available, and could find and use in the websites. K-M plotter (http://kmplot.com/), GEPIA (http://gepia.cancer-pku.cn/), and GSE62229 and GSE15459 (https://www.ncbi.nlm.nih.gov/gds)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:33739392"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE15459"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE15459"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:33739392", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1042/BSR20204293", "sha256": "sha256:864f2b671fd603936d8f327c81f5386a1484f85633b1b9e3e1471e93de0f3046", "bytes": 29584, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1042/BSR20204293", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC8039095", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE15459", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE15459", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE15459", "version": null, "note": "All data in this article were available, and could find and use in the websites. 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