Identification of Metabolic-Associated Genes for the Prediction of Colon and Rectal Adenocarcinoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:33833525", "kind": "science-record", "revision": 7, "content_hash": "sha256:0c9be49aaadfdacff6ea80cf24a341adaa734dda496d049b5ccf2f3a1ae26823", "created_at": "2026-11-01T15:41:31Z", "updated_at": "2026-11-01T22:03:31Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identification of Metabolic-Associated Genes for the Prediction of Colon and Rectal Adenocarcinoma.", "doi": "10.2147/OTT.S297134", "pmid": "33833525", "authors": ["Yanfen Cui", "Baoai Han", "He Zhang", "Hui Liu", "Fei Zhang", "Ruifang Niu"], "journal": "OncoTargets and therapy", "year": 2021, "abstract": "BACKGROUND AND AIM: Uncontrolled proliferation is the most prominent biological feature of tumors. In order to rapidly proliferate, tumor cells regulate their metabolic behavior by controlling the expression of metabolism-related genes (MRGs) to maximize the utilization of available nutrients. In this study, we aimed to construct prognosis models for colorectal adenocarcinoma (COAD) and rectum adenocarcinoma (READ) using MRGs to predict the prognoses of patients.\nMETHODS: We first acquired the gene expression profiles of COAD and READ from the TCGA database, and then utilized univariate Cox analysis, Lasso regression, and multivariable Cox analysis to identify the MRGs for risk models.\nRESULTS: Eight genes (CPT1C, PLCB2, PLA2G2D, GAMT, ENPP2, PIP4K2B, GPX3, and GSR) in the colon cancer risk model and six genes (TDO2, PKLR, GAMT, EARS2, ACO1, and WAS) in the rectal cancer risk model were identified successfully. Multivariate Cox analysis indicated that these two models could accurately and independently predict overall survival (OS) for patients with COAD or READ. Furthermore, functional enrichment analysis was used to identify the metabolism pathway of MRGs in the risk models and analyzed these genes comprehensively. Then, we verified the prognosis model in independent COAD cohorts (GSE17538) and detected the correlations of the protein expression levels of GSR and ENPP2 with prognosis for COAD or READ.\nCONCLUSION: In this study, 14 MRGs were identified as potential prognostic biomarkers and therapeutic targets for colorectal cancer.", "claims": [{"id": "claim-data-access", "statement": "The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33833525", "locator": "sections[24] \"Data Sharing Statement\" paragraph 2", "quote": "The datasets used or analyzed during the current study are available from the corresponding author on reasonable request."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:33833525"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE17538"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "blocked", "blocked_on": "method:group_comparison_wilcoxon", "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE17538"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": ["step:run_analysis blocked on method:group_comparison_wilcoxon"]}, "data": {"state": "partial", "assets": [{"id": "source:pmid:33833525", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.2147/OTT.S297134", "sha256": "sha256:686b5bb622512b76374bd19c6495e4d42bc9c8f4e118145958987f95d2e148c5", "bytes": 41021, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.2147/OTT.S297134", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC8020594", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE17538", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE17538", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE17538", "version": null, "note": "The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM437093", "GSM437094", "GSM437095", "GSM437096", "GSM437097", "GSM437098", "GSM437099", "GSM437100", "GSM437101", "GSM437102", "GSM437103", "GSM437104", "GSM437105", "GSM437106", "GSM437107", "GSM437108", "GSM437109", "GSM437110", "GSM437111", "GSM437112", "GSM437113", "GSM437114", "GSM437115", "GSM437116", "GSM437117", "GSM437118", "GSM437119", "GSM437120", "GSM437121", "GSM437122", "GSM437123", "GSM437124", "GSM437125", "GSM437126", "GSM437127", "GSM437128", "GSM437129", "GSM437130", "GSM437131", "GSM437132", "GSM437133", "GSM437134", "GSM437135", "GSM437136", "GSM437137", "GSM437138", "GSM437139", "GSM437140", "GSM437141", "GSM437142", "GSM437143", "GSM437144", "GSM437145", "GSM437146", "GSM437147", "GSM437148", "GSM437149", "GSM437150", "GSM437151", "GSM437152", "GSM437153", "GSM437154", "GSM437155", "GSM437156", "GSM437157", "GSM437158", "GSM437159", "GSM437160", "GSM437161", "GSM437162", "GSM437163", "GSM437164", "GSM437165", "GSM437166", "GSM437167", "GSM437168", "GSM437169", "GSM437170", "GSM437171", "GSM437172", "GSM437173", "GSM437174", "GSM437175", "GSM437176", "GSM437177", "GSM437178", "GSM437179", "GSM437180", "GSM437181", "GSM437182", "GSM437183", "GSM437184", "GSM437185", "GSM437186", "GSM437187", "GSM437188", "GSM437189", "GSM437190", "GSM437191", "GSM437192", "GSM437193", "GSM437194", "GSM437195", "GSM437196", "GSM437197", "GSM437198", "GSM437199", "GSM437200", "GSM437201", "GSM437202", "GSM437203", "GSM437204", "GSM437205", "GSM437206", "GSM437207", "GSM437208", "GSM437209", "GSM437210", "GSM437211", "GSM437212", "GSM437213", "GSM437214", "GSM437215", "GSM437216", "GSM437217", "GSM437218", "GSM437219", "GSM437220", "GSM437221", "GSM437222", "GSM437223", "GSM437224", "GSM437225", "GSM437226", "GSM437227", "GSM437228", "GSM437229", "GSM437230", "GSM437231", "GSM437232", "GSM437233", "GSM437234", "GSM437235", "GSM437236", "GSM437237", "GSM437238", "GSM437239", "GSM437240", "GSM437241", "GSM437242", "GSM437243", "GSM437244", "GSM437245", "GSM437246", "GSM437247", "GSM437248", "GSM437249", "GSM437250", "GSM437251", "GSM437252", "GSM437253", "GSM437254", "GSM437255", "GSM437256", "GSM437257", "GSM437258", "GSM437259", "GSM437260", "GSM437261", "GSM437262", "GSM437263", "GSM437264", "GSM437265", "GSM437266", "GSM437267", "GSM437268", "GSM437269", "GSM437270", "GSM437271", "GSM437272", "GSM437273", "GSM437274", "GSM437275", "GSM437276", "GSM437277", "GSM437278", "GSM437279", "GSM437280", "GSM437281", "GSM437282", "GSM437283", "GSM437284", "GSM437285", "GSM437286", "GSM437287", "GSM437288", "GSM437289", "GSM437290", "GSM437291", "GSM437292", "GSM437293", "GSM437294", "GSM437295", "GSM437296", "GSM437297", "GSM437298", "GSM437299", "GSM437300", "GSM437301", "GSM437302", "GSM437303", "GSM437304", "GSM437305", "GSM437306", "GSM437307", "GSM437308", "GSM437309", "GSM437310", "GSM437311", "GSM437312", "GSM437313", "GSM437314", "GSM437315", "GSM437316", "GSM437317", "GSM437318", "GSM437319", "GSM437320", "GSM437321", "GSM437322", "GSM437323", "GSM437324", "GSM472192", "GSM472193", "GSM472194", "GSM472195", "GSM472196", "GSM472197", "GSM472198", "GSM472199", "GSM472200", "GSM472201", "GSM472202", "GSM472203"], "sample_count": 244, "group_sizes": null, "group_sizes_basis": null, "platform": "570;1261", "taxon": "Mus musculus; Homo sapiens", "title": "Experimentally Derived Metastasis Gene Expression Profile Predicts Recurrence and Death in Colon Cancer Patients", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "asset:table:pmid:33833525:1", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:d6b87c52d5a332eddda0f81cd59e3357f1c3ef4a0e0d5b249409c76beaaa18e5", "bytes": 1222, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[3]/table-wrap[1]#t0001", "version": null, "note": "Clinicopathological Parameters of COAD Patients in the TCGA Database", "structure": {"pmcid": "PMC8020594", "section_index": 3, "section_title": "Data Collection", "row_count": 31, "column_count": 5}, "table": {"label": "Table 1", "caption": "Clinicopathological Parameters of COAD Patients in the TCGA Database", "rows": [["Variables", "COAD", "READ"], ["n", "Percentages (%)", "n", "Percentages (%)"], ["Age(years)", "", "", "", ""], ["<60", "104", "27.08", "28", "32.94"], ["≥60", "280", "72.92", "57", "67.06"], ["Gender", "", "", "", ""], ["Male", "204", "53.12", "48", "56.47"], ["Female", "180", "46.88", "37", "43.53"], ["T", "", "", "", ""], ["T1", "9", "2.34", "4", "4.71"], ["T2", "68", "17.71", "17", "20.00"], ["T3", "263", "68.49", "58", "68.23"], ["T4", "44", "11.46", "6", "7.06"], ["N", "", "", "", ""], ["N0", "230", "59.90", "46", "54.12"], ["N1", "88", "22.92", "25", "29.41"], ["N2", "66", "17.18", "13", "15.29"], ["Nx", "0", "0", "1", "1.18"], ["M", "", "", "", ""], ["M0", "286", "74.48", "65", "76.47"], ["M1", "54", "14.06", "13", "15.29"], ["Mx", "44", "11.46", "7", "8.24"], ["Clinical stage", "", "", "", ""], ["Stage I", "65", "16.94", "17", "20.00"], ["Stage II", "151", "39.32", "27", "31.76"], ["Stage III", "103", "26.82", "23", "27.06"], ["Stage IV", "54", "14.06", "14", "16.47"], ["Stage x", "11", "2.86", "4", "4.71"], ["Survival status", "", "", "", ""], ["Alive", "314", "81.77", "75", "88.24"], ["Dead", "70", "18.23", "10", "11.76"]], "row_count": 31, "column_count": 5, "numeric_cells": 90, "header_rows": 2, "locator": "section[3]/table-wrap[1]#t0001"}}, {"id": "asset:table:pmid:33833525:2", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:aaa97802452ca43d610f8267030d2696f3d381278d90e4863bacdaa892b05f82", "bytes": 957, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[8]/table-wrap[1]#t0002", "version": null, "note": "Clinicopathological Parameters of COAD and READ Patients", "structure": {"pmcid": "PMC8020594", "section_index": 8, "section_title": "Patient Samples", "row_count": 23, "column_count": 5}, "table": {"label": "Table 2", "caption": "Clinicopathological Parameters of COAD and READ Patients", "rows": [["Variables", "COAD", "READ"], ["n", "Percentages (%)", "n", "Percentages (%)"], ["Age(years)", "", "", "", ""], ["<60", "69", "58.94", "55", "48.25"], ["≥60", "48", "41.03", "59", "51.75"], ["Gender", "", "", "", ""], ["Male", "66", "56.41", "47", "41.23"], ["Female", "51", "43.59", "67", "58.77"], ["T", "", "", "", ""], ["T1–2", "21", "17.95", "15", "13.16"], ["T3–4", "96", "82.05", "99", "86.84"], ["N", "", "", "", ""], ["N0", "72", "61.54", "71", "62.28"], ["N1–2", "45", "38.46", "43", "37.72"], ["M", "", "", "", ""], ["M0", "114", "97.44", "112", "98.25"], ["M1", "3", "2.56", "2", "1.75"], ["Clinical stage", "", "", "", ""], ["Stage I–II", "80", "68.38", "65", "57.02"], ["Stage III–IV", "37", "31.62", "49", "42.98"], ["Survival status", "", "", "", ""], ["Alive", "106", "90.60", "99", "86.84"], ["Dead", "11", "9.40", "15", "13.16"]], "row_count": 23, "column_count": 5, "numeric_cells": 61, "header_rows": 2, "locator": "section[8]/table-wrap[1]#t0002"}}, {"id": "asset:table:pmid:33833525:3", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:fb5847d077305dce764c43938ac1fbaa47192b96e4bc94df6878d4779129a1b9", "bytes": 973, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[14]/table-wrap[1]#t0003", "version": null, "note": "Lasso Cox Analysis for colon cancer", "structure": {"pmcid": "PMC8020594", "section_index": 14, "section_title": "Construction and Verification of the COAD Risk Model", "row_count": 9, "column_count": 4}, "table": {"label": "Table 3", "caption": "Lasso Cox Analysis for colon cancer", "rows": [["Gene", "Full Name of Gene", "Coefficient", "Metabolism-Related KEGG PAthways"], ["ENPP2", "Ectonucleotide pyrophosphatase/phosphodiesterase 2", "0.0415", "Ether lipid metabolism"], ["GPX3", "Glutathione peroxidase 3", "0.0069", "Glutathione metabolism"], ["CPT1C", "Carnitine O-palmitoyltransferase 1", "0.0889", "Fatty acid degradation"], ["PLA2G2D", "Phospholipase A2 group IID", "0.0145", "Glycerphospholipid metabolism; Ether lipid metabolism"], ["GAMT", "Guanidinoacetate N-methyltransferase", "0.0957", "Glycine, serine and threonine metabolism"], ["PIP4K2B", "Phosphatidylinositol-5-phosphate 4-kinase type 2 beta", "0.0552", "Inositol phosphate metabolism"], ["PLCB2", "Phospholipase C beta 2", "0.1001", "Inositol phosphate metabolism"], ["GSR", "Glutathione metabolism", "−0.0179", "Glutathione metabolism"]], "row_count": 9, "column_count": 4, "numeric_cells": 13, "header_rows": 1, "locator": "section[14]/table-wrap[1]#t0003"}}, {"id": "asset:table:pmid:33833525:4", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:b5c4a9c66b672b48b575fb7ce9a13205955386cf8494b40a875363af37ad977d", "bytes": 725, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[15]/table-wrap[1]#t0004", "version": null, "note": "Lasso Cox Analysis for rectal cancer", "structure": {"pmcid": "PMC8020594", "section_index": 15, "section_title": "Construction of the READ Risk Model", "row_count": 7, "column_count": 4}, "table": {"label": "Table 4", "caption": "Lasso Cox Analysis for rectal cancer", "rows": [["Gene", "Full Name of Gene", "Coefficient", "Metabolism-Related KEGG Pathways"], ["TDO2", "Tryptophan 2,3-dioxygenase", "0.1041", "Tryptophan metabolism"], ["PKLR", "Pyruvate kinase isozymes R/L", "0.1239", "Pyruvate metabolism"], ["GAMT", "Guanidinoacetate N-methyltransferase", "0.0211", "Glycine, serine and threonine metabolism"], ["EARS2", "Glutamyl-tRNA synthetase 2", "−0.2970", "Porphyrin and chlorophyll metabolism"], ["ACO1", "Aconitase 1", "−0.2775", "TCA cycle"], ["WARS", "Tryptophanyl-tRNA synthetase", "−0.0097", "Aminoacyl-tRNA biosynthesis"]], "row_count": 7, "column_count": 4, "numeric_cells": 9, "header_rows": 1, "locator": "section[15]/table-wrap[1]#t0004"}}], "gaps": ["asset:data:geo:GSE17538 not downloaded", "asset:data:geo:GSE17538 licence not read"]}, "analysis": {"state": "partial", "methods": [{"id": "method-1", "family": "group_comparison", "specific": "wilcoxon", "label": "Group comparison", "dsl_operations": [], "missing_operations": ["group_comparison_wilcoxon"], "expressible": false, "parameters": {}, "data_shapes": ["two_or_more_groups_continuous"], "evidence": {"score": 0.8, "band": "high", "quote": "Wilcoxon"}}, {"id": "method-2", "family": "survival_analysis", "specific": "cox_proportional_hazards", "label": "Survival analysis", "dsl_operations": ["cox_ph"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["time_to_event_with_censoring"], "evidence": {"score": 0.8, "band": "high", "quote": "Cox regression"}}, {"id": "method-3", "family": "survival_analysis", "specific": "log_rank_test", "label": "Survival analysis", "dsl_operations": ["logrank_test"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["time_to_event_with_censoring"], "evidence": {"score": 0.8, "band": "high", "quote": "Log Rank"}}, {"id": "method-4", "family": "diagnostic_evaluation", "specific": "roc", "label": "Diagnostic test evaluation", "dsl_operations": ["confusion_counts", "roc_curve", "sensitivity_specificity", "solve_confusion_matrix", "classification_metrics", "published_precision_envelope"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["reference_standard_and_test"], "evidence": {"score": 0.8, "band": "high", "quote": "ROC analysis"}}], "targets": [], "gaps": ["method.group_comparison_wilcoxon"]}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", "candidate": "Automated Assays Laravel application and simulated adapters"}], "gaps": []}, "readiness": {"state": "complete", "tier": "R1", "blocking_axis": "none", "blocked_on": "nothing:replicable_now", "axes": {"method": {"tier": "R1", "expressible": false, "required_operations": [], "missing_operations": ["group_comparison_wilcoxon"], "basis": "The paper names the method it used and the corpus's method extractor maps it onto the DSL catalogue: wilcoxon."}, "capability": {"tier": "R1", "capability_id": "capability:computational_analysis", "availability_state": "owned", "basis": "The computational analysis is owned. The physical bench the paper used is not, so a physical validation cannot be run here."}, "data": {"tier": "R1", "reachable": true, "accessions": ["GSE17538"], "resolved_accessions": ["GSE17538"], "statements": ["The datasets used or analyzed during the current study are available from the corresponding author on reasonable request."], "basis": "The paper names GSE17538 in Gene Expression Omnibus (GEO) and the accession resolves in the repository. No dataset was downloaded and no licence was read."}}, "can_run": {"dsl_operations": [], "assets": ["source:pmid:33833525", "data:geo:GSE17538", "asset:table:pmid:33833525:1", "asset:table:pmid:33833525:2", "asset:table:pmid:33833525:3", "asset:table:pmid:33833525:4"], "steps": []}, "must_build": {"dsl_operations": [{"operation": "group_comparison_wilcoxon", "unlocks_papers": null, "priority": null}], "converters": [], "readers": [], "capabilities": [], "data_gaps": ["data:geo:GSE17538"]}, "gaps": []}, "review": {"state": "partial", "machine_critique": {"kind": "extraction_review", "reader": {"model": "MiniMax-M3.1-Flash", "provider": "minimax", "provider_family": "minimax", "read_at": "2026-10-31T19:30:47Z", "independence": "single_reader"}, "evidence_check": {"method": "absent", "availability": "verbatim"}, "calls": 3, "usage": {"prompt_tokens": 29646, "completion_tokens": 1787, "cached_tokens": 528}}, "human_critique": null, "stages": [{"id": "extraction", "state": "complete", "reader": "MiniMax-M3.1-Flash", "provider": "minimax"}], "gaps": ["review.independent_reader", "review.machine_critique", "review.human_critique"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "pmid:33833525", "type": "paper", "label": "pmid:33833525"}, {"id": "source:pmid:33833525", "type": "manuscript", "label": "PMID 33833525 full text"}, {"id": "data:geo:GSE17538", "type": "dataset", "label": "GEO GSE17538"}], "activities": [{"id": "activity-reading-sci-2026-11-01", "type": "classification", "used": [{"id": "pmid:33833525", "type": "paper", "label": "pmid:33833525"}, {"id": "source:pmid:33833525", "type": "manuscript", "label": "PMID 33833525 full text"}, {"id": "data:geo:GSE17538", "type": "dataset", "label": "GEO GSE17538"}], "agent": "agent:corpus-assay-engine"}, {"id": "activity-read-33833525", "type": "extraction", "used": ["source:pmid:33833525"], "agent": "agent:minimax-reader"}, {"id": "activity-resolve-GSE17538", "type": "accession_resolution", "used": ["data:geo:GSE17538"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}, {"id": "agent:minimax-reader", "type": "software", "label": "MiniMax literature reader"}], "source_run_id": "reading-sci-2026-11-01", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "pmid:33833525", "parent_hash": "sha256:d55efa6bffd24c086c0b0949e8c411bc3b177b8b98eb5f69748459308307b274", "fork_of": null, "generation": 6}, "gaps": ["asset:data:geo:GSE17538 licence not read", "asset:data:geo:GSE17538 not downloaded", "execution.not_run", "method.group_comparison_wilcoxon", "review.human_critique", "review.independent_reader", "review.machine_critique", "step:run_analysis blocked on method:group_comparison_wilcoxon"], "assays": [{"role": "analysis", "definition_id": "group_comparison", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 0.8, "evidence": ["Wilcoxon"], "order_basis": "analysis_only", "id": "assay:1"}]}