Dynamic Intracellular Metabolic Cell Signaling Profiles During Ag-Dependent B-Cell Differentiation.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:33859640", "kind": "science-record", "revision": 8, "content_hash": "sha256:e73b5e047205c33ad064296c8c0e9d4744d2ed29bcf74d2cb3461e9849b5a65d", "created_at": "2026-11-01T15:41:32Z", "updated_at": "2026-11-01T22:46:31Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Dynamic Intracellular Metabolic Cell Signaling Profiles During Ag-Dependent B-Cell Differentiation.", "doi": "10.3389/fimmu.2021.637832", "pmid": "33859640", "authors": ["Paula Díez", "Martín Pérez-Andrés", "Martin Bøgsted", "Mikel Azkargorta", "Rodrigo García-Valiente", "Rosa M Dégano", "Elena Blanco", "Sheila Mateos-Gomez", "Paloma Bárcena", "Santiago Santa Cruz", "Rafael Góngora", "Félix Elortza", "Alicia Landeira-Viñuela", "Pablo Juanes-Velasco", "Victor Segura", "Raúl Manzano-Román", "Julia Almeida", "Karen Dybkaer", "Alberto Orfao", "Manuel Fuentes"], "journal": "Frontiers in immunology", "year": 2021, "abstract": "Human B-cell differentiation has been extensively investigated on genomic and transcriptomic grounds; however, no studies have accomplished so far detailed analysis of antigen-dependent maturation-associated human B-cell populations from a proteomic perspective. Here, we investigate for the first time the quantitative proteomic profiles of B-cells undergoing antigen-dependent maturation using a label-free LC-MS/MS approach applied on 5 purified B-cell subpopulations (naive, centroblasts, centrocytes, memory and plasma B-cells) from human tonsils (data are available via ProteomeXchange with identifier PXD006191). Our results revealed that the actual differences among these B-cell subpopulations are a combination of expression of a few maturation stage-specific proteins within each B-cell subset and maturation-associated changes in relative protein expression levels, which are related with metabolic regulation. The considerable overlap of the proteome of the 5 studied B-cell subsets strengthens the key role of the regulation of the stoichiometry of molecules associated with metabolic regulation and programming, among other signaling cascades (such as antigen recognition and presentation and cell survival) crucial for the transition between each B-cell maturation stage.", "claims": [{"id": "claim-method", "statement": "Using the Progenesis suite, an ANOVA test with a q-value cutoff of 0.05 followed up by individual T-tests was used to determinate which proteins discriminated among different cell populations.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33859640", "locator": "sections[11] \"Quantitative Protein Expression Levels\" paragraph 2", "quote": "Using the Progenesis suite, an ANOVA test with a q-value cutoff of 0.05 followed up by individual T-tests was used to determinate which proteins discriminated among different cell populations."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The full mass spectrometry proteomics data reported in this paper has been deposited to ProteomeXchange Consortium via the PRIDE (65) partner repository with the dataset identifier PXD006191.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33859640", "locator": "sections[23] \"Data Availability Statement\" paragraph 2", "quote": "The full mass spectrometry proteomics data reported in this paper has been deposited to ProteomeXchange Consortium via the PRIDE (65) partner repository with the dataset identifier PXD006191."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:33859640"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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