The mechanism of TiaoGanYiPi formula for treating chronic hepatitis B by network pharmacology and molecular docking verification.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:33863948", "kind": "science-record", "revision": 6, "content_hash": "sha256:7229a460de22727b628f6b117294ffefc6dc0f935a22bdc77c5e60deb7f6f21a", "created_at": "2026-11-01T15:41:32Z", "updated_at": "2026-11-01T21:25:09Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "The mechanism of TiaoGanYiPi formula for treating chronic hepatitis B by network pharmacology and molecular docking verification.", "doi": "10.1038/s41598-021-87812-9", "pmid": "33863948", "authors": ["Xu Cao", "Xiaobin Zao", "Baiquan Xue", "Hening Chen", "Jiaxin Zhang", "Shuo Li", "Xiaobin Li", "Shun Zhu", "Rui Guo", "Xiaoke Li", "Yong'an Ye"], "journal": "Scientific reports", "year": 2021, "abstract": "The Chinese herbal formula TiaoGanYiPi (TGYP) showed effective against chronic hepatitis B (CHB) caused by hepatitis B virus (HBV) infection. Hence, we aimed to clarify the mechanisms and potential targets between TGYP and CHB. The active compounds and related putative targets of TGYP, and disease targets of CHB were obtained from the public databases. The key targets between TGYP and CHB were identified through the network construction and module analysis. The expression of the key targets was detected in Gene Expression Omnibus (GEO) dataset and normal hepatocyte cell line LO2. We first obtained 11 key targets which were predominantly enriched in the Cancer, Cell cycle and HBV-related pathways. And the expression of the key targets was related to HBV infection and liver inflammation verified in GSE83148 database. Furthermore, the results of real-time quantitative PCR and CCK-8 assay indicated that TGYP could regulate the expression of key targets including CCNA2, ABL1, CDK4, CDKN1A, IGFR and MAP2K1, and promote proliferation of LO2 cells. In coclusion, we identified the active compounds and key targets btween TGYP and CHB, and found that the TGYP might exhibite curative effect on CHB via promoting hepatocyte proliferation and inhibiting the liver inflammatory processes.", "claims": [{"id": "claim-method", "statement": "For the comparison of quantitative data between groups, the Student’s t-test is used.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33863948", "locator": "sections[10] \"Expression of the key target genes in CHB liver tissues and correlation with liver inflammation\" paragraph 2", "quote": "For the comparison of quantitative data between groups, the Student’s t-test is used."}], "state": "complete"}, {"id": "claim-data-access", "statement": "All the relevant data is provided within the paper and its supporting information files. 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{"label": "Table 1", "caption": "The docking scores of key compounds and key target genes.", "rows": [["Targets", "PBD ID", "Compounds", "Affinity (kcal/mol)", "Baseline affinity (ETV, kcal/mol)"], ["ABL1", "6BL8", "( +)-Anomalin", "−8.4", "−6.9"], ["CASP8", "6PX9", "Baicalin", "−8.1", "−5.7"], ["CASP8", "6PX9", "Cubebin", "−7.1", "−5.7"], ["CCNA2", "1E9H", "corycavine", "−10.1", "−7.6"], ["CCNB1", "6GU2", "Calycosin", "−7.9", "−6.7"], ["CCNB1", "6GU2", "quercetin", "−7.5", "−6.7"], ["CCNB1", "6GU2", "Isorhamnetin", "−7.6", "−6.7"], ["CCNB1", "6GU2", "Kaempferol", "−8.0", "−6.7"], ["CDK4", "5FWP", "( +)-Anomalin", "−8.3", "−9.4"], ["CDKN1A", "6P8H", "Calycosin", "−6.6", "−5.5"], ["EP300", "6PF1", "(3R)-3-(2-hydroxy-3,4-dimethoxyphenyl)chroman-7-ol", "−8.2", "−6.9"], ["HIF1A", "3HQU", "quercetin", "−7.7", "−7.1"], ["HIF1A", "3HQU", "Saikogenin F", "−6.8", "−7.1"], ["IGF1R", "3D94", "quercetin", "−8.3", "−6.9"], ["IGF1R", "3D94", "kaempferol", "−8.2", "−6.9"], ["IGF1R", "3D94", 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