Overexpression of CRABP2 inhibits dexamethasone-induced apoptosis in human osteoblast cells.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:33879199", "kind": "science-record", "revision": 8, "content_hash": "sha256:17cf478f12d20655d7cf0dd1cd6da4dbadd056019e900bdabd309163d3c07c25", "created_at": "2026-11-01T15:41:33Z", "updated_at": "2026-11-01T22:46:33Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Overexpression of CRABP2 inhibits dexamethasone-induced apoptosis in human osteoblast cells.", "doi": "10.1186/s13018-021-02386-6", "pmid": "33879199", "authors": ["Haiping Zhang", "Ziliang Yu", "Farui Sun", "Jin Jin"], "journal": "Journal of orthopaedic surgery and research", "year": 2021, "abstract": "BACKGROUND: The purpose of the current study was to explore the role and underlying mechanism of cellular retinoic acid binding protein 2 (CRABP2) in dexamethasone (DEX)-induced apoptosis in human osteoblast cells.\nMETHODS: GSE10311 was downloaded from the Gene Expression Omnibus (GEO) database to identify the differentially expressed genes (DEGs) by the limma/R package. Primary human osteoblast was isolated and treated with different concentration of DEX (0, 10-8, 10-7, 10-6, 10-5, and 10-4 mol/L), and cell viability and flow cytometry were used to detect cell proliferation and apoptosis. A CRABP2 overexpression plasmid (oe-CRABP2) was used to overexpress CRABP2, and western blotting was conducted to detect protein expression.\nRESULTS: We found that CRABP2 was downregulated in the DEX-treated group. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses indicated that DEGs were associated with PI3K/Akt signaling pathway. DEX downregulated CRABP2 gene and protein expression, inhibited viability, and induced human osteoblast apoptosis. Overexpression of CRABP2 reversed DEX-induced apoptosis in human osteoblast. Moreover, overexpression of CRABP2 delayed the progression of DEX-induced osteonecrosis of the femoral head (ONFH) animal model.\nCONCLUSION: In conclusion, CRABP2 is effective at inhibiting DEX-induced human osteoblast apoptosis and delayed ONFH progression.", "claims": [{"id": "claim-method", "statement": "Differences among different groups were calculated with one-way analysis of variance (ANOVA) and post-analysis by Tukey’s honestly significant difference test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33879199", "locator": "sections[11] \"Statistical analysis\" paragraph 2", "quote": "Differences among different groups were calculated with one-way analysis of variance (ANOVA) and post-analysis by Tukey’s honestly significant difference test."}], "state": "complete"}, {"id": "claim-data-access", "statement": "We state that the data will not be shared since all the raw data are present in the figures included in the article.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33879199", "locator": "sections[23] \"notes\" paragraph 1", "quote": "We state that the data will not be shared since all the raw data are present in the figures included in the article."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:33879199"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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METHODS: GSE10311", "y were used to detect cell proliferation and apoptosis. A CRABP2 overexpression plasmid (oe-CRABP2)", "ited viability, and induced human osteoblast apoptosis. Overexpression of CRABP2 reversed DEX-induc"], "order_basis": "paper_text_order:11", "id": "assay:1"}, {"role": "data_generation", "definition_id": "flow_cytometry", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["0-5, and 10-4 mol/L), and cell viability and flow cytometry were used to detect cell proliferation and a"], "order_basis": "paper_text_order:576", "id": "assay:2"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["-CRABP2) was used to overexpress CRABP2, and western blotting was conducted to detect protein expression."], "order_basis": "paper_text_order:725", "id": "assay:3"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["terials and methods Cell culture and plasmid transfection The human osteoblast was isolated and cultur", "oe-CRABP2) was synthesized by Santa Cruz and transfected following the manufacturer’s protocol. Then,", "dish (NEST, Hong Kong, China) for transient transfection. DMEM (1 mL) containing 10% FBS was added to", "nd the cells were cultured for 24 h prior to transfection. Transfection was performed using Lipofectam"], "order_basis": "paper_text_order:1491", "id": "assay:4"}, {"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["wley rats weighing 320 ± 30 g were used. The rats were randomly and equally subdivided into five gr", "fter the next 4 weeks. Five weeks later, the rats were euthanized, and the femoral heads were exami", "NFH + CRABP2-pcDNA3+LY294002. To induce ONFH in rats, pulsed high-dose 100 mg/(kg•d) MPS was intr", "Twenty-four 8-week-old female Sprague-Dawley rats weighing 320 ± 30 g were used. The rats were"], "order_basis": "paper_text_order:2907", "id": "assay:5"}, {"role": "data_generation", "definition_id": "ihc", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["ration with CRABP2 overexpression plasmid. b Immunohistochemistry results of the COL1A expression in femoral h"], "order_basis": "paper_text_order:4883", "id": "assay:6"}, {"role": "analysis", "definition_id": "group_comparison", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["group_summary", "welch_t", "bootstrap_mean_difference", "group_comparison_anova"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Differences among different groups were calculated with one-way analysis of variance (ANOVA) and post-analysis by Tukey’s honestly significant difference test."], "order_basis": "consumes_earlier_output", "id": "assay:7"}]}