Decreased HLF Expression Predicts Poor Survival in Lung Adenocarcinoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:33979320", "kind": "science-record", "revision": 7, "content_hash": "sha256:6b78e9c825f731012e2d09c4ba50d1de37cb8a0a4313c83b88f348c2a36debaa", "created_at": "2026-11-01T15:41:35Z", "updated_at": "2026-11-01T22:03:44Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Decreased HLF Expression Predicts Poor Survival in Lung Adenocarcinoma.", "doi": "10.12659/MSM.929333", "pmid": "33979320", "authors": ["Zaiyan Wang", "Xiaoning Li", "Hao Chen", "Li Han", "Xiaobin Ji", "Qiubo Wang", "Li Wei", "Yafang Miao", "Jing Wang", "Jianfeng Mao", "Zeming Zhang"], "journal": "Medical science monitor : international medical journal of experimental and clinical research", "year": 2021, "abstract": "BACKGROUND Lung adenocarcinoma (LUAD) is a type of non-small cell carcinoma. Its pathogenesis is being explored and there is no cure for the disease. MATERIAL AND METHODS The Gene Expression Omnibus (GEO) was searched to obtain data on expression of messenger RNA. GEO2R, an interactive web tool, was used to calculate the differentially expressed genes (DEGs) in LUAD. All the DEGs from different datasets were imported into VENNY 2.1 (https://bioinfogp.cnb.csic.es/tools/venny/index.html) to identify the intersection of the DEGs. An online analysis tool, the Database for Annotation, Visualization, and Integrated Discovery (DAVID), was used to help understand the biological meaning of DEG enrichment in LUAD. Cytoscape 3.7.2 was used to perform centrality analysis and visualize hub genes and related networks. Furthermore, the prognostic value of the hub genes was evaluated with the Kaplan-Meier plotter survival analysis tool. RESULTS The GEO database was used to obtain RNA sequencing information for LUAD and normal tissue from the GSE118370, GSE136043, and GSE140797 datasets. A total of 376 DEGs were identified from GSE118370, 248 were identified from GSE136403, and 718 DEGs were identified from GSE140797. The 10 genes with the highest degrees of expression - the hub genes - were CAV1, TEK, SLIT2, RHOJ, DGSX, HLF, MEIS1, PTPRD, FOXF1, and ADRB2. In addition, Kaplan-Meier survival evaluation showed that CAV1, TEK, SLIT2, HLF, MEIS1, PTPRD, FOXF1, and ADRB2 were associated with favorable outcomes for LUAD. CONCLUSIONS CAV1, TEK, SLIT2, HLF, MEIS1, PTPRD, FOXF1, and ADRB2 are hub genes in the DEG interaction network for LUAD and are involved in the development of and prognosis for the disease. The mechanisms underlying these genes should be the subject of further studies.", "claims": [{"id": "claim-method", "statement": "GEO2R is online software used to analyze data from GEO.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:33979320", "locator": "sections[4] \"Identification of Differentially Expressed Genes\" paragraph 2", "quote": "GEO2R is online software used to analyze data from GEO."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:33979320"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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Cell Culture and Transfection The LUAD cell line A549 was purchased from t", "culture all the cells at 37°C in 5% CO2. For transfection, the negative control small interfering RNA", "arma Biotechnology Co., Ltd. A549 cells were transfected with 100 nM NC siRNA or HLA siRNA using Lipo", "the negative control small interfering RNA (siRNA) and HLF siRNA were designed by Suzhou GeneP"], "order_basis": "paper_text_order:3046", "id": "assay:2"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["Culture and Transfection The LUAD cell line A549 was purchased from the Shanghai Cell Bank. D", "by Suzhou GenePharma Biotechnology Co., Ltd. A549 cells were transfected with 100 nM NC siRNA", "lementary Figure 1Inhibition of HLF promotes A549 cell proliferation. (A) qRT-PCR results of t", "(B) qRT-PCR results of the HLF expression in A549 cells after siRNA-NC and siRNA-HLF treatment"], "order_basis": "paper_text_order:3079", "id": "assay:3"}, {"role": "analysis", "definition_id": "survival_analysis", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["survival_km"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 0.7, "evidence": ["Kaplan-Meier"], "order_basis": "consumes_earlier_output", "id": "assay:4"}]}