Clinical implications of TPO and AOX1 in pediatric papillary thyroid carcinoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34012822", "kind": "science-record", "revision": 4, "content_hash": "sha256:906bf43e1be4d392a0fdab21de66639e965a5ea12a3dad100e497ca342bfd2ed", "created_at": "2026-11-01T15:41:36Z", "updated_at": "2026-11-01T21:25:22Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Clinical implications of TPO and AOX1 in pediatric papillary thyroid carcinoma.", "doi": "10.21037/tp-20-301", "pmid": "34012822", "authors": ["Yongbo Yu", "Shengcai Wang", "Xuexi Zhang", "Shuai Xu", "Yanzhen Li", "Qiaoyin Liu", "Yeran Yang", "Nian Sun", "Yuanhu Liu", "Jie Zhang", "Yongli Guo", "Xin Ni"], "journal": "Translational pediatrics", "year": 2021, "abstract": "BACKGROUND: Thyroid carcinoma is a common pediatric head and neck cancer, of which papillary thyroid cancer (PTC) is the most common type. Previously, we found that thyroid peroxidase (TPO) and aldehyde oxidase 1 (AOX1) were differentially expressed in PTC. This study explored the clinical importance of TPO and AOX1 in the diagnosis and prognosis of PTC in children.\nMETHODS: Both TPO and AOX1 expression in PTC were analyzed using datasets from Gene Expression Omnibus (GEO). TPO and AOX1 protein levels in plasma from patients with PTC and non-tumor controls were detected via enzyme-linked immunosorbent assay (ELISA). The diagnostic accuracy of TPO and AOX1 was assessed using receiver operating characteristic (ROC) curve analysis. The association between gene expression levels and patient survival was explored using the Kaplan-Meier plotter online database.\nRESULTS: The results revealed that TPO and AOX1 expression was significantly downregulated in four independent datasets (GSE33630, GSE27155, GSE3678, and GSE3467). TPO and AOX1 protein levels in blood plasma were significantly decreased in patients with PTC. Quantitative analysis demonstrated that TPO and AOX1 levels in plasma had satisfactory predictive performance and the ability to discriminate PTC from healthy samples. Prognostic analysis demonstrated that low levels of TPO and AOX1 were markedly associated with poor survival in patients with PTC.\nCONCLUSIONS: In summary, these results implied that TPO and AOX1 could serve as novel biomarkers for the diagnosis and prognosis of pediatric PTC.", "claims": [{"id": "claim-method", "statement": "An unpaired t-test was performed to analyze differences between two groups.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34012822", "locator": "sections[8] \"Statistical analysis\" paragraph 2", "quote": "An unpaired t-test was performed to analyze differences between two groups."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data Sharing Statement: Available at http://dx.doi.org/10.21037/tp-20-301", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34012822", "locator": "sections[18] \"Footnotes\" paragraph 2", "quote": "Data Sharing Statement: Available at http://dx.doi.org/10.21037/tp-20-301"}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34012822"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE27155"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE27155"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:34012822", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.21037/tp-20-301", "sha256": 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