Quantitative proteomic comparison of salt stress in Chlamydomonas reinhardtii and the snow alga Chlamydomonas nivalis reveals mechanisms for salt-triggered fatty acid accumulation via reallocation of carbon resources.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34022944", "kind": "science-record", "revision": 8, "content_hash": "sha256:8cf011d09cf47280c2fe4e93758118c1c4f291d4d6fc6c91edb33c9af5d01bd0", "created_at": "2026-11-01T15:41:36Z", "updated_at": "2026-11-01T22:46:44Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Quantitative proteomic comparison of salt stress in Chlamydomonas reinhardtii and the snow alga Chlamydomonas nivalis reveals mechanisms for salt-triggered fatty acid accumulation via reallocation of carbon resources.", "doi": "10.1186/s13068-021-01970-6", "pmid": "34022944", "authors": ["E Hounslow", "C A Evans", "J Pandhal", "T Sydney", "N Couto", "T K Pham", "D James Gilmour", "P C Wright"], "journal": "Biotechnology for biofuels", "year": 2021, "abstract": "BACKGROUND: Chlamydomonas reinhardtii is a model green alga strain for molecular studies; its fully sequenced genome has enabled omic-based analyses that have been applied to better understand its metabolic responses to stress. Here, we characterised physiological and proteomic changes between a low-starch C. reinhardtii strain and the snow alga Chlamydomonas nivalis, to reveal insights into their contrasting responses to salinity stress.\nRESULTS: Each strain was grown in conditions tailored to their growth requirements to encourage maximal fatty acid (as a proxy measure of lipid) production, with internal controls to allow comparison points. In 0.2 M NaCl, C. nivalis accumulates carbohydrates up to 10.4% DCW at 80 h, and fatty acids up to 52.0% dry cell weight (DCW) over 12 days, however, C. reinhardtii does not show fatty acid accumulation over time, and shows limited carbohydrate accumulation up to 5.5% DCW. Analysis of the C. nivalis fatty acid profiles showed that salt stress improved the biofuel qualities over time. Photosynthesis and respiration rates are reduced in C. reinhardtii relative to C. nivalis in response to 0.2 M NaCl. De novo sequencing and homology matching was used in conjunction with iTRAQ-based quantitative analysis to identify and relatively quantify proteomic alterations in cells exposed to salt stress. There were abundance differences in proteins associated with stress, photosynthesis, carbohydrate and lipid metabolism proteins. In terms of lipid synthesis, salt stress induced an increase in dihydrolipoyl dehydrogenase in C. nivalis (1.1-fold change), whilst levels in C. reinhardtii remained unaffected; this enzyme is involved in acetyl CoA production and has been linked to TAG accumulation in microalgae. In salt-stressed C. nivalis there were decreases in the abundance of UDP-sulfoquinovose (- 1.77-fold change), which is involved in sulfoquinovosyl diacylglycerol metabolism, and in citrate synthase (- 2.7-fold change), also involved in the TCA cycle. Decreases in these enzymes have been shown to lead to increased TAG production as fatty acid biosynthesis is favoured. Data are available via ProteomeXchange with identifier PXD018148.\nCONCLUSIONS: These differences in protein abundance have given greater understanding of the mechanism by which salt stress promotes fatty acid accumulation in the un-sequenced microalga C. nivalis as it switches to a non-growth state, whereas C. reinhardtii does not have this response.", "claims": [{"id": "claim-method", "statement": "Proteins that were statistically different in abundance using ANOVA (p < 0.05) across the treatments are summarised in Tables 2 and 3 for C. reinhardtii and C. nivalis, respectively.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34022944", "locator": "sections[5] \"Proteomic study in C. reinhardtii and C. nivalis\" paragraph 4", "quote": "Proteins that were statistically different in abundance using ANOVA (p < 0.05) across the treatments are summarised in Tables 2 and 3 for C. reinhardtii and C. nivalis, respectively."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE [83] partner repository with the dataset identifier PXD018148.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34022944", "locator": "sections[28] \"notes\" paragraph 1", "quote": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE [83] partner repository with the dataset identifier PXD018148."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34022944"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:PXD018148"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:PXD018148"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:34022944", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1186/s13068-021-01970-6", "sha256": "sha256:cea47f2cec0f29885d7253b33ccbeef727400c2332affd37878919c32e6e9b15", "bytes": 81812, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1186/s13068-021-01970-6", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC8141184", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:PXD018148", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=PXD018148", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "PXD018148", "version": null, "note": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE [83] partner repository with the dataset identifier PXD018148.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE [83] partner repository with the dataset identifier PXD018148.", "access_statement_kind": "data_availability_statement", "sample_accessions": [], "sample_count": null, "group_sizes": null, "group_sizes_basis": null, "platform": null, "taxon": null, "title": "¬¬Quantitative proteomic comparison of salt stress in Chlamydomonas reinhardtii and snow alga Chlamydomonas nivalis reveals mechanisms for salt-triggered lipid accumulation via reallocation of carbon resources", "resolution_checked_at": "2026-10-31T18:35:55Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "asset:table:pmid:34022944:1", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:2bbf570066b79956e61d95c41cd0a2b387f46e77af26a73ba0415ee0425c17f8", "bytes": 1469, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[3]/table-wrap[1]#Tab1", "version": null, "note": "Biofuel properties of C. nivalis samples (n = 3)", "structure": {"pmcid": "PMC8141184", "section_index": 3, "section_title": "The impact of salt stress on the physiology of Chlamydomonas reinhardtii and Chlamydomonas nivalis", "row_count": 12, "column_count": 8}, "table": {"label": "Table 1", "caption": "Biofuel properties of C. nivalis samples (n = 3)", "rows": [["Sample", "Saponification number (mg KOH g−1 oil)", "Iodine number (g I2 100 g−1 oil)", "Cetane number", "Degree of unsaturation (wt. %)", "Long chain saturation factor (LCSF) (°C)", "Cold filter plugging point (CFPP) (°C)"], ["Time (hours)", "NaCl concentration (M)", "", "", "", ""], ["0", "0", "193.6 ± 0.1", "144.5 ± 0.4", "42.0 ± 0.1", "117.2 ± 0.4", "12.2 ± 0.5", "21.9 ± 1.7"], ["0", "0.2", "193.5 ± 0.0", "143.8 ± 0.2", "42.1 ± 0.1", "115.7 ± 0.2", "13.1 ± 0.1", "24.6 ± 0.3"], ["24", "0", "193.8 ± 0.2", "145.5 ± 0.5", "41.7 ± 0.1", "117.2 ± 0.8", "12.5 ± 0.4", "22.7 ± 1.2"], ["24", "0.2", "193.8 ± 0.1", "131.5 ± 1.2", "44.9 ± 0.3", "110.6 ± 1.1", "11.0 ± 0.2", "18.2 ± 0.8"], ["80", "0", "193.6 ± 0.1", "144.2 ± 0.7", "42.0 ± 0.1", "117.9 ± 0.3", "11.6 ± 0.3", "20.0 ± 0.8"], ["80", "0.2", "192.4 ± 0.1", "115.9 ± 0.8", "48.6 ± 0.2", "107.2 ± 0.4", "6.8 ± 0.2", "4.8 ± 0.7"], ["168", "0", "194.1 ± 0.1", "132.1 ± 0.5", "44.7 ± 0.1", "113.0 ± 0.3", "10.1 ± 0.1", "15.2 ± 0.5"], ["168", "0.2", "191.8 ± 0.1", "104.9 ± 0.4", "51.2 ± 0.1", "104.1 ± 0.1", "4.2 ± 0.1", "− 3.4 ± 0.4"], ["291", "0", "193.6 ± 0.3", "115.5 ± 1.5", "48.5 ± 0.4", "107.2 ± 0.7", "6.3 ± 0.3", "3.4 ± 0.9"], ["291", "0.2", "191.6 ± 0.1", "98.8 ± 0.8", "52.6 ± 0.2", "101.1 ± 0.7", "3.5 ± 0.2", "− 5.4 ± 0.8"]], "row_count": 12, "column_count": 8, "numeric_cells": 82, "header_rows": 2, "locator": "section[3]/table-wrap[1]#Tab1"}}, {"id": "asset:table:pmid:34022944:2", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:d2ec8d920e34dfba22637e23000a6aed44fb998b8b1871d8a77c522ee4c4b57c", "bytes": 557, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[5]/table-wrap[1]#Tab2", "version": null, "note": "Summary of protein changes detected between sample groups in C. reinhardtii iTRAQ experiment", "structure": {"pmcid": "PMC8141184", "section_index": 5, "section_title": "Proteomic study in C. reinhardtii and C. nivalis", "row_count": 5, "column_count": 4}, "table": {"label": "Table 2", "caption": "Summary of protein changes detected between sample groups in C. reinhardtii iTRAQ experiment", "rows": [["Comparison", "Number of proteins changed", "Number of proteins increased", "Number of proteins decreased"], ["0 h and 11 h salt conditions", "171", "91", "80"], ["11 h and 18 h salt conditions", "217", "94", "123"], ["0 h and 18 h salt conditions", "212", "76", "136"], ["18 h control and 18 h salt conditions", "337", "113", "224"]], "row_count": 5, "column_count": 4, "numeric_cells": 16, "header_rows": 1, "locator": "section[5]/table-wrap[1]#Tab2"}}, {"id": "asset:table:pmid:34022944:3", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:6a879397bb54be01c38598082e2c3dba07f6291cf1541cc2c29445b6d282a954", "bytes": 555, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[5]/table-wrap[2]#Tab3", "version": null, "note": "Summary of protein changes detected between sample groups in C. nivalis iTRAQ experiment", "structure": {"pmcid": "PMC8141184", "section_index": 5, "section_title": "Proteomic study in C. reinhardtii and C. nivalis", "row_count": 5, "column_count": 4}, "table": {"label": "Table 3", "caption": "Summary of protein changes detected between sample groups in C. nivalis iTRAQ experiment", "rows": [["Comparison", "Number of proteins changed", "Number of proteins increased", "Number of proteins decreased"], ["0 h and 80 h salt conditions", "143", "115", "28"], ["80 h and 168 h salt conditions", "22", "12", "10"], ["0 h and 168 h salt conditions", "155", "26", "129"], ["168 h control and 168 h salt conditions", "231", "43", "188"]], "row_count": 5, "column_count": 4, "numeric_cells": 16, "header_rows": 1, "locator": "section[5]/table-wrap[2]#Tab3"}}, {"id": "asset:table:pmid:34022944:4", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:a02f1973347649b36ea91d5a595b12e82c48ec59f7d77dd8a5c18f5f83b1b8af", "bytes": 11680, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[5]/table-wrap[3]#Tab4", "version": null, "note": "Summary of protein changes in each strain during salt stress", "structure": {"pmcid": "PMC8141184", "section_index": 5, "section_title": "Proteomic study in C. reinhardtii and C. nivalis", "row_count": 74, "column_count": 11}, "table": {"label": "Table 4", "caption": "Summary of protein changes in each strain during salt stress", "rows": [["Protein accession ID", "Protein name", "Pathway/role", "Salt stress treatment comparisons with relative protein changes (values indicate fold change)"], ["", "", "", "C. reinhardtii", "C. nivalis"], ["", "", "", "0–11 h salt", "11–18 h salt", "0–18 h salt", "18 h control–18 h salt", "0–80 h salt", "80–168 h salt", "0–168 h salt", "168 h control–168 h salt"], ["Stress proteins"], ["A8HYV3/ Q39603", "Heat shock protein 70B", "Chloroplast localised heat shock protein protecting against photoinhibition [84]", "↑ 1.073", "→", "↑ 1.077", "→", "↓ -1.340", "→", "↓ -1.363", "↓ -1.708"], ["A8J3P5", "Heat shock protein 70E", "Cytosolic heat shock protein [84]", "→", "→", "→", "↓ -1.172", "→", "→", "→", "→"], ["A8JEU4", "Heat shock protein 70A", "Cytosolic heat shock protein [84]", "→", "→", "→", "→", "↓ -1.358", "→", "↓ -1.334", "↓ -1.880"], ["A8IZU0", "Heat shock protein 70C", "Mitochondrial heat shock protein [84]", "→", "→", "→", "→", "↓ -1.336", "→", "↓ -1.386", "↓ -1.731"], ["A8J1U1", "Heat shock protein 90A", "Cytosolic heat shock protein [85]", "→", "→", "→", "→", "→", "→", "↓ -1.189", "↓ -1.848"], ["Q66T67", "Heat shock protein 90C", "Chloroplastic heat shock protein [85]", "→", "→", "→", "→", "→", "→", "↓ -1.543", "↓ -1.868"], ["A8I7T1", "Heat shock protein 90B (Fragment)", "ER localised heat shock protein [86]", "→", "→", "→", "→", "→", "→", "→", "↓ -1.771"], ["Q1RS84", "Aldehyde-alcohol dehydrogenase", "Aldehyde scavenging (during lipid peroxidation) [42]", "↑ 1.134", "→", "↑ 1.106", "→", "→", "→", "→", "→"], ["A8JHR9/ A8JHS0/ P49644", "Glyceraldehyde-3-phosphate dehydrogenase", "Glycolysis (and carbohydrate metabolism)", "→", "↑ 1.155", "↑ 1.284", "→", "↑ 2.166", "→", "↑ 2.200", "→"], ["A8HXA6", "Glutathione reductase", "Reduction of glutathione disulphide [45]", "↓ -1.195", "↑ 1.201", "→", "→", "→", "↑ 1.277", "→", "→"], ["A8JG06", "Programmed cell death protein 6-interacting protein", "Protein self-association and ubiquitin binding", "→", "→", "→", "→", "→", "↑ 1.208", "→", "→"], ["A8J1C1", "Ubiquitin-activating enzyme E1", "Ubiquitin conjugation cascade", "↓ -1.088", "↑ 1.169", "→", "→", "→", "↑ 1.254", "↑ 1.198", "↑ 1.097"], ["A8J5Z8", "Aldehyde dehydrogenase", "Aldehyde detoxification, ROS scavenging, inhibits lipid peroxidation", "→", "↑ 1.180", "↑ 1.274", "↑ 1.271", "→", "→", "↑ 1.306", "→"], ["A8JB85", "Autophagy-related protein", "Autophagy", "↑ 1.385", "→", "↑ 1.274", "→", "→", "→", "↑ 1.352", "→"], ["A8J431", "Stress-related chlorophyll a/b binding protein 2", "Non-photochemical quenching, protect photosynthetic apparatus from photo-oxidative damage", "→", "→", "→", "↑ 1.776", "→", "→", "↑ 1.572", "→"], ["Photosynthesis proteins"], ["Q39588", "Carbonic anhydrase, alpha type", "Carbon dioxide fixation [87]", "→", "→", "↓ -1.202", "↓ -1.135", "→", "→", "→", "→"], ["Q8S567", "Chlorophyll a/b-binding protein", "Photosynthesis and light harvesting", "↓ -1.190", "→", "↓ -1.191", "→", "→", "→", "→", "→"], ["A8J270", "Chlorophyll a-b binding protein of LHCII", "Light harvesting in photosystem I", "→", "→", "→", "↑ 1.144", "→", "→", "→", "→"], ["Q8S3T9", "Chlorophyll a-b binding protein of LHCII", "Light harvesting in photosystem I", "→", "→", "→", "↑ 1.171", "→", "→", "→", "→"], ["A8HP84/ P50362", "Glyceraldehyde-3-phosphate dehydrogenase", "Photosynthesis, Calvin cycle", "→", "→", "→", "→", "↓ -1.529", "→", "↓ -1.682", "↓ -2.147"], ["Q75VY7", "Light-harvesting chlorophyll-a/b protein of photosystem I", "Chlorophyll binding, light harvesting in photosystem I", "↓ -1.240", "→", "→", "↓ -1.347", "→", "→", "→", "→"], ["A8ITV3", "Light-harvesting protein of photosystem I", "Chlorophyll binding, light harvesting in photosystem I", "↓ -1.190", "→", "↓ -1.191", "→", "→", "→", "→", "→"], ["Q93VE0", "Light-harvesting chlorophyll-a/b binding protein LhcII-4", "Chlorophyll binding, light harvesting in photosystem I", "→", "→", "→", "↑ 1.147", "→", "→", "→", "→"], ["A8J0B1", "Low-CO2-inducible protein", "Inorganic carbon uptake [52]", "↓ -1.161", "→", "→", "→", "→", "→", "→", "→"], ["A8J225", "Low-CO2-inducible protein (Fragment)", "Inorganic carbon uptake [52]", "↑ 1.285", "→", "↑ 1.240", "↑ 1.385", "→", "→", "→", "→"], ["Q75NZ1", "Low-CO2 inducible protein", "Inorganic carbon uptake [52]", "→", "↑ 1.067", "→", "→", "→", "→", "→", "→"], ["A8IT08", "Low-CO2-inducible chloroplast envelope protein", "L-orthinine transmembrane transport activity", "→", "→", "↑ 1.268", "↑ 1.390", "→", "→", "→", "→"], ["A8JEV1", "Oxygen evolving enhancer protein 3", "Photosynthetic electron transfer chain, calcium ion binding", "→", "↓ -1.150", "↓ -1.141", "↓ -1.226", "↓ -1.495", "→", "↓ -1.674", "→"], ["A8J0E4", "Oxygen-evolving enhancer protein 1 of photosystem II", "Photosystem II assembly and stability, oxygen evolving activity", "→", "↓ -1.094", "↓ -1.145", "↓ -1.290", "↓ -1.864", "→", "↓ -2.186", "↓ -2.300"], ["A8IYH9", "Oxygen-evolving enhancer protein 2 of photosystem II", "Photosynthesis, calcium ion binding", "→", "→", "↓ -1.136", "↓ -1.108", "↓ -1.520", "→", "↓ -1.626", "↓ -2.072"], ["P12852", "Oxygen-evolving enhancer protein 3, chloroplastic", "Photosynthesis, calcium ion binding", "→", "↓ -1.150", "↓ -1.141", "↓ -1.226", "↓ -1.495", "→", "↓ -1.674", "→"], ["Q00914", "Photosystem I iron-sulphur centre", "Photosynthetic electron transport in photosystem I", "→", "→", "→", "→", "↓ -1.672", "→", "↓ -1.932", "→"], ["P13352", "Photosystem I reaction centre subunit VI, chloroplastic", "Photosynthesis", "→", "→", "→", "→", "→", "→", "→", "↓ -2.285"], ["Q5NKW4", "Photosystem I reaction centre subunit II, 20 kDa", "Photosynthesis", "→", "↓ -1.177", "→", "→", "→", "→", "→", "→"], ["Q39615", "Photosystem I reaction centre subunit II, chloroplastic", "Photosynthesis", "→", "↓ -1.177", "→", "→", "→", "→", "→", "→"], ["P12154", "Photosystem I P700 chlorophyll a apoprotein A1", "Photosynthesis, electron transfer, chlorophyll binding", "→", "→", "↓ -1.261", "→", "↓ -1.823", "→", "↓ -1.864", "→"], ["P09144", "Photosystem I P700 chlorophyll a apoprotein A2", "Photosynthesis, electron transfer, chlorophyll binding", "→", "→", "↓ -1.200", "→", "↓ -1.643", "→", "↓ -1.614", "→"], ["P10898", "Photosystem II CP43 reaction centre protein", "Photosynthetic electron transport in photosystem II, chlorophyll binding", "→", "→", "→", "→", "↓ -1.623", "→", "↓ -1.824", "↓ -1.469"], ["P37255", "Photosystem II CP47 reaction centre protein", "Photosynthetic electron transport in photosystem II, chlorophyll binding", "→", "→", "→", "→", "↓ -1.583", "→", "↓ -1.693", "→"], ["P06007", "Photosystem II D2 protein", "Photosynthetic electron transport in photosystem II, chlorophyll binding", "→", "→", "→", "→", "↓ -1.611", "→", "↓ -1.634", "→"], ["P07753", "Photosystem II protein D1", "Photosynthetic electron transport in photosystem II, chlorophyll binding", "→", "→", "→", "→", "↓ -1.840", "→", "↓ -1.727", "↓ -1.445"], ["A8J4S1", "Photosystem I reaction centre subunit III", "Photosynthesis, protein domain specific binding", "→", "↓ -1.255", "↓ -1.289", "↓ -1.461", "→", "→", "→", "→"], ["P12356", "Photosystem I reaction centre subunit III, chloroplastic", "Photosynthesis, protein domain specific binding", "→", "↓ -1.255", "↓ -1.289", "↓ -1.461", "→", "→", "→", "→"], ["A8HVJ9", "Photosystem II stability/assembly factor HCF136 fragment", "Photosystem II biogenesis", "→", "→", "→", "↓ -1.190", "→", "→", "→", "→"], ["A8JGP9", "Ribulose bisphosphate carboxylase small chain", "Carbon fixation, photorespiration, photosynthesis", "↓ -1.213", "→", "↓ -1.356", "→", "→", "→", "→", "→"], ["P00873", "Ribulose bisphosphate carboxylase small chain 1, chloroplastic", "Carbon fixation, photorespiration, photosynthesis", "↓ -1.213", "→", "↓ -1.356", "→", "→", "→", "→", "→"], ["P08475", "Ribulose bisphosphate carboxylase small chain 2, chloroplastic", "Carbon fixation, photorespiration, photosynthesis", "↓ -1.219", "→", "↓ -1.357", "→", "→", "→", "→", "→"], ["P00877", "Ribulose bisphosphate carboxylase large chain", "Photorespiration, reductive pentose-phosphate cycle", "→", "↓ -1.078", "↓ -1.154", "↓ -1.110", "↓ -1.810", "→", "↓ -1.991", "↓ -2.751"], ["P23489", "Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic", "Photosynthesis regulation", "→", "↓ -1.107", "↓ -1.108", "↓ -1.186", "→", "→", "↓ -1.555", "↓ -2.809"], ["Q6SA05", "Rubisco activase", "Photosynthesis regulation", "→", "↓ -1.107", "↓ -1.108", "→", "→", "→", "↓ -1.555", "↓ -2.809"], ["Carbohydrate proteins"], ["Q2VA40", "Alpha-1,4 glucan phosphorylase", "Starch degradation [88]", "↑ 1.123", "↑ 1.105", "↑ 1.240", "↑ 1.122", "↑ 1.237", "↑ 1.158", "↑ 1.433", "↓ -1.688"], ["Q2VA41/ A8IYK1", "Alpha-1,4 glucan phosphorylase", "Starch degradation [88]", "↑ 1.096", "→", "↑ 1.147", "→", "→", "→", "→", "→"], ["A2PZC2", "UDP-glucose:protein transglucosylase", "Polysaccharide biosynthesis", "→", "→", "→", "→", "↓ -1.535", "→", "↓ -1.713", "↓ -2.510"], ["A8HW52", "Starch branching enzyme", "Starch biosynthesis", "→", "→", "→", "→", "→", "→", "→", "↓ -2.318"], ["A8IHX1", "Starch branching enzyme", "Starch biosynthesis", "→", "→", "↑ 1.207", "→", "→", "→", "→", "→"], ["A8J914", "UDP-glucose 6-dehydrogenase", "Starch catabolism for glycolysis [58]", "↑ 1.263", "↑ 1.239", "↑ 1.562", "↑ 1.478", "→", "→", "→", "→"], ["A2PZC3", "UDP-glucose 6-dehydrogenase", "Starch catabolism for glycolysis [58]", "↑ 1.298", "↑ 1.231", "↑ 1.595", "↑ 1.461", "→", "→", "→", "↑ 2.008"], ["Lipid proteins"], ["A8HNN4/ A0A0B5KTL4", "Glycerol-3-phosphate dehydrogenase", "Glycerol metabolism, link between carbohydrate and lipid metabolism", "↑ 1.675", "↑ 1.169", "↑ 1.959", "↑ 1.959", "↑ 2.135", "↑ 1.192", "↑ 2.544", "↑ 2.648"], ["A0A0B5KYA7", "Glycerol-3-phosphate dehydrogenase", "Glycerol metabolism, link between carbohydrate and lipid metabolism", "↑ 1.719", "↑ 1.179", "↑ 2.027", "↑ 2.064", "→", "→", "→", "→"], ["A8HNN6", "Glycerol-3-phosphate dehydrogenase (fragment)", "Glycerol metabolism, link between carbohydrate and lipid metabolism", "↑ 1.744", "↑ 1.189", "↑ 2.073", "↑ 2.109", "→", "→", "→", "→"], ["A8J6J6", "Acetyl-CoA acyltransferase", "Catabolic beta-oxidation of fatty acids [63]", "↑ 1.162", "→", "↑ 1.181", "↑ 1.209", "→", "→", "↑ 1.250", "↑ 1.375"], ["A8J2S0", "Citrate synthase", "Tricarboxylic acid cycle", "→", "→", "→", "→", "→", "→", "→", "↓ -2.725"], ["Q6DN05", "Betaine lipid synthase", "Betaine lipid synthesis, including DGTS synthesis", "→", "↓ -1.093", "→", "→", "→", "→", "→", "→"], ["A8HXT4", "Pyruvate carboxylase", "Pyruvate metabolism, gluconeogenesis, links carbohydrate and lipid metabolism", "→", "↓ -1.047", "↓ -1.100", "→", "→", "→", "→", "→"], ["A8JGF4", "Biotin carboxylase, acetyl-CoA carboxylase component", "Malonyl-CoA biosynthesis, lipid metabolism", "→", "→", "→", "↓ -1.113", "↓ -1.479", "→", "↓ -1.482", "↓ -1.860"], ["Q763T6", "UDP-sulfoquinovose synthase", "Glycerolipid metabolism, sulfoquinovosyl diacylglycerol (SQDG) metabolism", "→", "→", "→", "→", "↓ -1.275", "→", "↓ -1.289", "↓ -1.770"], ["A8J1T4", "Dihydrolipoyl dehydrogenase", "Acetyl CoA production, cell redox homeostasis", "→", "→", "→", "→", "↑ 1.110", "→", "→", "↑ 1.149"]], "row_count": 74, "column_count": 11, "numeric_cells": 285, "header_rows": 3, "locator": "section[5]/table-wrap[3]#Tab4"}}], "gaps": ["asset:data:geo:PXD018148 not downloaded", "asset:data:geo:PXD018148 licence not read"]}, "analysis": {"state": "complete", "methods": [{"id": "method-1", "family": "group_comparison", "specific": "anova", "label": "Group comparison", "dsl_operations": ["group_summary", "welch_t", 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