Comparative Analysis of Bursaphelenchus xylophilus Secretome Under Pinus pinaster and P. pinea Stimuli.

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In order to study B. xylophilus secretomes under the stimulus of pine species with different susceptibilities to disease, nematodes were exposed to aqueous pine extracts from Pinus pinaster (high-susceptible host) and P. pinea (low-susceptible host). Sequential windowed acquisition of all theoretical mass spectra (SWATH-MS) was used to determine relative changes in protein amounts between B. xylophilus secretions, and a total of 776 secreted proteins were quantified in both secretomes. From these, 22 proteins were found increased in the B. xylophilus secretome under the P. pinaster stimulus and 501 proteins increased under the P. pinea stimulus. Functional analyses of the 22 proteins found increased in the P. pinaster stimulus showed that proteins with peptidase, hydrolase, and antioxidant activities were the most represented. On the other hand, gene ontology (GO) enrichment analysis of the 501 proteins increased under the P. pinea stimulus revealed an enrichment of proteins with binding activity. The differences detected in the secretomes highlighted the diverse responses from the nematode to overcome host defenses with different susceptibilities and provide new clues on the mechanism behind the pathogenicity of this plant-parasitic nematode. Proteomic data are available via ProteomeXchange with identifier PXD024011.", "claims": [{"id": "claim-method", "statement": "Statistical analysis was carried out to identify the differentially regulated proteins using a Mann–Whitney U test to the proteins that fulfill these criteria, with a q value of 0.05 as cutoff performed in InfernoRDN (version 1.1.5581.33355) (Polpitiya et al., 2008).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34046053", "locator": "sections[6] \"Protein Quantification by Sequential Windowed Acquisition of All Theoretical Mass Spectra\" paragraph 8", "quote": "Statistical analysis was carried out to identify the differentially regulated proteins using a Mann–Whitney U test to the proteins that fulfill these criteria, with a q value of 0.05 as cutoff performed in InfernoRDN (version 1.1.5581.33355) (Polpitiya et al., 2008)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets presented in this study can be found in online repositories. 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"Description of increased proteins in Bursaphelenchus xylophilus secretome under the Pinus pinaster stimulus, based on molecular function gene ontology terms.", "structure": {"pmcid": "PMC8144518", "section_index": 10, "section_title": "Differential Quantitative Analysis of Secreted Proteins Under Different Stimuli", "row_count": 23, "column_count": 4}, "table": {"label": "TABLE 1", "caption": "Description of increased proteins in Bursaphelenchus xylophilus secretome under the Pinus pinaster stimulus, based on molecular function gene ontology terms.", "rows": [["Activity", "Annotation", "No. of proteins", "Protein ID"], ["Peptidase", "Aspartic peptidase", "4", "BXY_0035000.1"], ["", "", "", "BXY_0555800.1"], ["", "", "", "BXY_0820600.1"], ["", "", "", "BXY_0821000.1"], ["", "Serine peptidase", "1", "BXY_0959000.1"], ["Hydrolase", "Acid sphingomyelinase", "1", "BXY_0542900.1"], ["", "Histidine acid phosphatase", "1", "BXY_1236200.1"], ["", "Lysozyme-like protein (GH18)", "1", 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"application/vnd.jats.table+json", "uri": null, "sha256": "sha256:b072902877c471d54524ceb283b6c1f1b717ecbe0b47a12cc73b0582a577973d", "bytes": 1890, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[10]/table-wrap[2]#T2", "version": null, "note": "Gene ontology (GO) enrichment analysis of the 501 proteins increased in Bursaphelenchus xylophilus secretome under the Pinus pinea stimulus.", "structure": {"pmcid": "PMC8144518", "section_index": 10, "section_title": "Differential Quantitative Analysis of Secreted Proteins Under Different Stimuli", "row_count": 23, "column_count": 6}, "table": {"label": "TABLE 2", "caption": "Gene ontology (GO) enrichment analysis of the 501 proteins increased in Bursaphelenchus xylophilus secretome under the Pinus pinea stimulus.", "rows": [["GO ID", "GO level", "GO name", "GO category", "P value", "No. of proteins"], ["GO:0005488", "2", "Binding", "MF", "2.77E-02", "202"], ["GO:0005515", "3", 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