High glucose treatment promotes extracellular matrix proteome remodeling in Mller glial cells.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34046254", "kind": "science-record", "revision": 7, "content_hash": "sha256:1093ab0b8b2bd91835169c38dd00f4ac63d39ea882a3b409b14085525b046727", "created_at": "2026-11-01T15:41:37Z", "updated_at": "2026-11-01T22:03:51Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "High glucose treatment promotes extracellular matrix proteome remodeling in Mller glial cells.", "doi": "10.7717/peerj.11316", "pmid": "34046254", "authors": ["Sandra Sagmeister", "Juliane Merl-Pham", "Agnese Petrera", "Cornelia A Deeg", "Stefanie M Hauck"], "journal": "PeerJ", "year": 2021, "abstract": "BACKGROUND: The underlying pathomechanisms in diabetic retinopathy (DR) remain incompletely understood. The aim of this study was to add to the current knowledge about the particular role of retinal Mller glial cells (RMG) in the initial processes of DR.\nMETHODS: Applying a quantitative proteomic workflow, we investigated changes of primary porcine RMG under short term high glucose treatment as well as glycolysis inhibition treatment.\nRESULTS: We revealed significant changes in RMG proteome primarily in proteins building the extracellular matrix (ECM) indicating fundamental remodeling processes of ECM as novel rapid response to high glucose treatment. Among others, Osteopontin (SPP1) as well as its interacting integrins were significantly downregulated and organotypic retinal explant culture confirmed the selective loss of SPP1 in RMG upon treatment. Since SPP1 in the retina has been described neuroprotective for photoreceptors and functions against experimentally induced cell swelling, its rapid loss under diabetic conditions may point to a direct involvement of RMG to the early neurodegenerative processes driving DR. Data are available via ProteomeXchange with identifier PXD015879.", "claims": [{"id": "claim-method", "statement": "To analyze the differential protein abundance between the diabetic treatment groups and the control group, a two-tailed, unpaired students t-test was performed on log2 transformed normalized abundances.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34046254", "locator": "sections[8] \"Statistics, graphics, principal component analysis, pathway enrichment analysis, volcano plots, Venn diagram and network generation\" paragraph 2", "quote": "To analyze the differential protein abundance between the diabetic treatment groups and the control group, a two-tailed, unpaired students t-test was performed on log2 transformed normalized abundances."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data are available via ProteomeXchange: PXD015879. https://www.ebi.ac.uk/pride/archive/projects/PXD015879.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34046254", "locator": "sections[20] \"Additional Information and Declarations\" paragraph 5", "quote": "Data are available via ProteomeXchange: PXD015879. https://www.ebi.ac.uk/pride/archive/projects/PXD015879."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34046254"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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