Construction and Analysis of a circRNA-Mediated ceRNA Network in Lung Adenocarcinoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34135596", "kind": "science-record", "revision": 5, "content_hash": "sha256:136d4b1ab798f4e48b46cf1815f6cfadafe21f62aa7ef0a244014ae7b92f698a", "created_at": "2026-11-01T15:41:39Z", "updated_at": "2026-11-01T21:25:32Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Construction and Analysis of a circRNA-Mediated ceRNA Network in Lung Adenocarcinoma.", "doi": "10.2147/OTT.S305030", "pmid": "34135596", "authors": ["Zhaojun Wang", "Hanzhong Pei", "Hongsen Liang", "Qiwei Zhang", "Li Wei", "Donglei Shi", "Yun Chen", "Junhang Zhang"], "journal": "OncoTargets and therapy", "year": 2021, "abstract": "BACKGROUND: Circular RNAs (circRNAs), a new class of regulatory noncoding RNAs, are involved in gene regulation and may play a role in cancer development. The aim of this study was to identify circRNAs involved in lung adenocarcinoma (LUAD) using bioinformatics analysis.\nMETHODS: CircRNA (GSE101684, GSE101586), miRNA (GSE135918), and mRNA (GSE130779) microarray datasets were downloaded from the Gene Expression Omnibus (GEO) database to identify differentially expressed circRNAs (DECs), miRNAs (DEMs), and mRNAs (DEGs) in LUAD. Circinteractome and StarBase were used to predict miRNAs and mRNAs, respectively. A circRNA-miRNA-mRNA-ceRNA network was constructed. Patient survival was analyzed using UALCAN, and a sub-network was established. Real-time quantitative PCR (qRT-PCR) was used to verify the expressed of DECs between LUAD tissues and paired adjacent normal tissues.\nRESULTS: Hsa_circ_0072088 was identified as a differentially expressed (upregulated) circRNA in the two datasets. Intersection analysis identified hsa-miR-532-3p and hsa-miR-942 as the two miRNAs with the highest potential for binding to hsa_circ_0072088. Differential expression analysis and target gene prediction were performed to build a ceRNA network of hsa_circ_0072088 using Circinteractome/StarBase 3.0. Intersection analysis showed that TMEM52, IL24, POF1B, KIF1A, NHS, LBH, HIST2H2BE, ABCC3, PYCR1, CD79A, IGF2BP3, ANKRD17, GTSE1, MKI67, CLSPN, PLAU, LUC7L, MAGIX, GPATCH4, and ABAT were potential downstream mRNAs. The association between the expression level of 20 DEGs and LUAD patient survival was analyzed using UALCAN and GEPIA, which showed that IGF2BP3, MKI67, CD79A, and ABAT were related to patient survival. Hsa_circ_0072088 was verified upregulated by qRT-PCR.\nCONCLUSION: The circRNA hsa_circ_0072088, the DEMs (hsa-miR-532-3p and hsa-miR-942-5p), and the DEGs (IGF2BP3, MKI67, CD79A, and ABAT) may serve as prognostic markers in LUAD.", "claims": [{"id": "claim-method", "statement": "Differential analyses of GSE101684, GSE101586, GSE135918, and GSE130779 were performed to identify DECs, DEMs, and DEGs between LUAD tissues and control normal tissues using the online tool GEO2R.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34135596", "locator": "sections[4] \"Identification of DECs, DEMs, and DEGs\" paragraph 2", "quote": "Differential analyses of GSE101684, GSE101586, GSE135918, and GSE130779 were performed to identify DECs, DEMs, and DEGs between LUAD tissues and control normal tissues using the online tool GEO2R."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The circRNA dataset GSE101684 included four LUAD tissues and four adjacent normal lung tissues, and the samples were tested by the GPL21825 074301 Arraystar Human CircRNA microarray V2 platform.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34135596", "locator": "sections[3] \"Source of Microarray Data\" paragraph 2", "quote": "The circRNA dataset GSE101684 included four LUAD tissues and four adjacent normal lung tissues, and the samples were tested by the GPL21825 074301 Arraystar Human CircRNA microarray V2 platform."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34135596"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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