Transcriptomic data of bovine ovarian granulosa cells of control and High A4 cows.

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The normalized linear microarray data was deposited to the NCBI GEO repository (GSE97017 - RNA Expression Data from Bovine Ovarian Granulosa Cells from High or Low Androgen-Content Follicles). Subsequent ANOVA determined genes that were enriched (≥ 1.5 fold more) or decreased (≤ 1.5 fold less) in the High A4 granulosa cells compared to Control granulosa cells and analyzed filtered datasets of these differentially expressed genes are presented as tables. MicroRNAs that are differentially expressed in Control and High A4 granulosa cells are also reported in tables. The standard deviation of the analyzed array data in relation to the log of the expression values are shown as a figure. Ingenuity Pathway Analysis determined upstream regulators of differently expressed genes as presented in a table. These data have been further analyzed and interpreted in the companion article \"A High-Androgen Microenvironment Inhibits Granulosa Cell Proliferation and Alters Cell Identity\" (McFee et. al., 2021 [1].", "claims": [{"id": "claim-method", "statement": "Array analysis was performed with normalized microarray results using the National Institute of Aging array tool (NIA) (http://lgsun.grc.nia.nih.gov/ANOVA/) for Analysis of Variance (ANOVA) to determine hierarchical clustering, and correlation between replicates (Fig. 1).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34189206", "locator": "sections[8] \"Data processing\" paragraph 2", "quote": "Array analysis was performed with normalized microarray results using the National Institute of Aging array tool (NIA) (http://lgsun.grc.nia.nih.gov/ANOVA/) for Analysis of Variance (ANOVA) to determine hierarchical clustering, and correlation between replicates (Fig. 1)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Full transcriptome data is available from the NCBI GEO repository, Series GSE97017.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34189206", "locator": "sections[7] \"Microarray analysis\" paragraph 2", "quote": "Full transcriptome data is available from the NCBI GEO repository, Series GSE97017."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34189206"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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