Circulating lncRNA UCA1 and lncRNA PGM5-AS1 act as potential diagnostic biomarkers for early-stage colorectal cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34212174", "kind": "science-record", "revision": 5, "content_hash": "sha256:c48ecc5bea315efc258200207139bbeb7283a0f170f4ad540c11f31e3c0e23a3", "created_at": "2026-11-01T15:41:41Z", "updated_at": "2026-11-01T22:04:06Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Circulating lncRNA UCA1 and lncRNA PGM5-AS1 act as potential diagnostic biomarkers for early-stage colorectal cancer.", "doi": "10.1042/BSR20211115", "pmid": "34212174", "authors": ["Minghui Wang", "Zhijun Zhang", "Deng Pan", "Zhigang Xin", "Fan Bu", "Yue Zhang", "Qingwu Tian", "Xiaodong Feng"], "journal": "Bioscience reports", "year": 2021, "abstract": "BACKGROUND: Colorectal cancer (CRC) is one of the most common and significant malignant diseases worldwide. In the present study, we evaluated two long non-coding RNAs (lncRNAs) in CRC patients as diagnostic markers for early-stage CRC.\nMETHODS: Using Gene Expression Omnibus (GEO) datasets GSE102340, GSE126092, GSE109454 and GSE115856, 14 differentially expressed lncRNAs were identified between cancer and adjacent tissues, among which, the two most differentially expressed were confirmed using quantitative real-time polymerase chain reaction (qRT-PCR) in 200 healthy controls and 188 CRC patients. A receiver operating characteristic (ROC) analysis was employed to evaluate the diagnostic accuracy for CRC.\nRESULTS: From four GEO datasets, three up-regulated and eleven down-regulated lncRNAs were identified in CRC tissues, among which, lncRNA urothelial carcinoma-associated 1 (UCA1) and lncRNA phosphoglucomutase 5-antisense RNA 1 (PGM5-AS1) were the most significantly up- and down-regulated lncRNAs in CRC patient plasma, respectively. The area under the ROC curve was calculated to be 0.766, 0.754 and 0.798 for UCA1, PGM5-AS1 and the combination of these two lncRNAs, respectively. Moreover, the diagnostic potential of these two lncRNAs was even higher for the early stages of CRC. The combination of UCA1 and PGM5-AS1 enhanced the AUC to 0.832, and when the lncRNAs were used with carcinoembryonic antigen (CEA), the AUC was further improved to 0.874.\nCONCLUSION: In the present study, we identified two lncRNAs, UCA1 and PGM5-AS1, in CRC patients' plasma, which have the potential to be used as diagnostic biomarkers of CRC.", "claims": [{"id": "claim-method", "statement": "The qRT-PCR results of the CRC patients were compared with those of the healthy individuals by the Mann–Whitney U test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34212174", "locator": "sections[7] \"Statistical analysis\" paragraph 2", "quote": "The qRT-PCR results of the CRC patients were compared with those of the healthy individuals by the Mann–Whitney U test."}], "state": "complete"}, {"id": "claim-data-access", "statement": "All data analysed in the present paper are already included in the manuscript, including two tables.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34212174", "locator": "sections[18] \"Data Availability\" paragraph 2", "quote": "All data analysed in the present paper are already included in the manuscript, including two tables."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34212174"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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