The proteolytic landscape of cells exposed to non-lethal stresses is shaped by executioner caspases.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34226511", "kind": "science-record", "revision": 4, "content_hash": "sha256:e0440700d756de26a6e2c00fe361d192723b32e63c21da637d04701b0aba5d33", "created_at": "2026-11-01T15:41:42Z", "updated_at": "2026-11-01T21:25:42Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "The proteolytic landscape of cells exposed to non-lethal stresses is shaped by executioner caspases.", "doi": "10.1038/s41420-021-00539-4", "pmid": "34226511", "authors": ["María Del Carmen Conde-Rubio", "Roman Mylonas", "Christian Widmann"], "journal": "Cell death discovery", "year": 2021, "abstract": "Cells are in constant adaptation to environmental changes to insure their proper functioning. When exposed to stresses, cells activate specific pathways to elicit adaptive modifications. Those changes can be mediated by selective modulation of gene and protein expression as well as by post-translational modifications, such as phosphorylation and proteolytic processing. Protein cleavage, as a controlled and limited post-translational modification, is involved in diverse physiological processes such as the maintenance of protein homeostasis, activation of repair pathways, apoptosis and the regulation of proliferation. Here we assessed by quantitative proteomics the proteolytic landscape in two cell lines subjected to low cisplatin concentrations used as a mild non-lethal stress paradigm. This landscape was compared to the one obtained in the same cells stimulated with cisplatin concentrations inducing apoptosis. These analyses were performed in wild-type cells and in cells lacking the two main executioner caspases: caspase-3 and caspase-7. Ninety-two proteins were found to be cleaved at one or a few sites (discrete cleavage) in low stress conditions compared to four hundred and fifty-three in apoptotic cells. Many of the cleaved proteins in stressed cells were also found to be cleaved in apoptotic conditions. As expected, ~90% of the cleavage events were dependent on caspase-3/caspase-7 in apoptotic cells. Strikingly, upon exposure to non-lethal stresses, no discrete cleavage was detected in cells lacking caspase-3 and caspase-7. This indicates that the proteolytic landscape in stressed viable cells fully depends on the activity of executioner caspases. These results suggest that the so-called executioner caspases fulfill important stress adaptive responses distinct from their role in apoptosis. 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Here we", "lated with cisplatin concentrations inducing apoptosis. These analyses were performed in wild-type", "aptive responses distinct from their role in apoptosis. Mass spectrometry data are available via Pr", "s lacking the two main executioner caspases: caspase-3 and caspase-7. Ninety-two proteins were fo"], "order_basis": "paper_text_order:634", "id": "assay:1"}, {"role": "data_generation", "definition_id": "mass_spectrometry", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["onses distinct from their role in apoptosis. Mass spectrometry data are available via ProteomeXchange with", "liferation. Here we assessed by quantitative proteomics the proteolytic landscape in two cell lines"], "order_basis": "paper_text_order:713", "id": "assay:2"}]}