Quantitative Proteomics Reveal That Metabolic Improvement Contributes to the Cardioprotective Effect of T89 on Isoproterenol-Induced Cardiac Injury.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34262469", "kind": "science-record", "revision": 8, "content_hash": "sha256:3b55fc93eeec898c379dd8be0423f4d69f21d4c0f140545167eec577ba30b3c7", "created_at": "2026-11-01T15:41:43Z", "updated_at": "2026-11-01T22:47:06Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Quantitative Proteomics Reveal That Metabolic Improvement Contributes to the Cardioprotective Effect of T89 on Isoproterenol-Induced Cardiac Injury.", "doi": "10.3389/fphys.2021.653349", "pmid": "34262469", "authors": ["Xiao-Hong Wei", "Xiao Guo", "Chun-Shui Pan", "Huan Li", "Yuan-Chen Cui", "Li Yan", "Jing-Yu Fan", "Jing-Na Deng", "Bai-He Hu", "Xin Chang", "Shu-Ya He", "Lu-Lu Yan", "Kai Sun", "Chuan-She Wang", "Jing-Yan Han"], "journal": "Frontiers in physiology", "year": 2021, "abstract": "BACKGROUND: T89, a traditional Chinese medicine, has passed phase II, and is undergoing phase III clinical trials for treatment of ischemic cardiovascular disease by the US FDA. However, the role of T89 on isoproterenol (ISO)-induced cardiac injury is unknown. The present study aimed to explore the effect and underlying mechanism of T89 on ISO-induced cardiac injury.\nMETHODS: Male Sprague-Dawley rats received subcutaneous injection of ISO saline solution at 24 h intervals for the first 3 days and then at 48 h intervals for the next 12 days. T89 at dose of 111.6 and 167.4 mg/kg was administrated by gavage for 15 consecutive days. Rat survival rate, cardiac function evaluation, morphological observation, quantitative proteomics, and Western blotting analysis were performed.\nRESULTS: T89 obviously improved ISO-induced low survival rate, attenuated ISO-evoked cardiac injury, as evidenced by myocardial blood flow, heart function, and morphology. Quantitative proteomics revealed that the cardioprotective effect of T89 relied on the regulation of metabolic pathways, including glycolipid metabolism and energy metabolism. T89 inhibited the enhancement of glycolysis, promoted fatty acid oxidation, and restored mitochondrial oxidative phosphorylation by regulating Eno1, Mcee, Bdh1, Ces1c, Apoc2, Decr1, Acaa2, Cbr4, ND2, Cox 6a, Cox17, ATP5g, and ATP5j, thus alleviated oxidative stress and energy metabolism disorder and ameliorated cardiac injury after ISO. The present study also verified that T89 significantly restrained ISO-induced increase of HSP70/HSP40 and suppressed the phosphorylation of ERK, further restored the expression of CX43, confirming the protective role of T89 in cardiac hypertrophy. Proteomics data are available via ProteomeXchange with identifier PXD024641.\nCONCLUSION: T89 reduced mortality and improves outcome in the model of ISO-induced cardiac injury and the cardioprotective role of T89 is correlated with the regulation of glycolipid metabolism, recovery of mitochondrial function, and improvement of myocardial energy.", "claims": [{"id": "claim-method", "statement": "Statistical analysis was performed using one-way ANOVA, followed by Bonferroni test for multiple comparisons.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34262469", "locator": "sections[15] \"Statistical Analysis\" paragraph 2", "quote": "Statistical analysis was performed using one-way ANOVA, followed by Bonferroni test for multiple comparisons."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets presented in this study can be found in online repositories. 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METHODS: Male Sprague-Dawley rats received subcutaneous injection of ISO salin", "nistrated by gavage for 15 consecutive days. Rat survival rate, cardiac function evaluation,", "., 2019). Animal Model and Treatments The SD rats were randomly divided into five groups: (1) Sham", "and Methods Animals Male Sprague-Dawley (SD) rats weighing 180–200 g were obtained from the Pe"], "order_basis": "paper_text_order:503", "id": "assay:1"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["al observation, quantitative proteomics, and Western blotting analysis were performed. RESULTS: T89 obviou"], "order_basis": "paper_text_order:845", "id": "assay:2"}, {"role": "analysis", "definition_id": "group_comparison", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["group_summary", "welch_t", "bootstrap_mean_difference", "group_comparison_anova"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Statistical analysis was performed using one-way ANOVA, followed by Bonferroni test for multiple comparisons."], "order_basis": "consumes_earlier_output", "id": "assay:3"}]}