Integrative Analysis of Differently Expressed Genes Reveals a 17-Gene Prognosis Signature for Endometrial Carcinoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34337010", "kind": "science-record", "revision": 8, "content_hash": "sha256:1ba8e4b50250090a151bc49b243396bf32c842645bdb6815e4f805df0e4bbe78", "created_at": "2026-11-01T15:41:46Z", "updated_at": "2026-11-01T22:47:15Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Integrative Analysis of Differently Expressed Genes Reveals a 17-Gene Prognosis Signature for Endometrial Carcinoma.", "doi": "10.1155/2021/4804694", "pmid": "34337010", "authors": ["Anna Wang", "Hongyan Guo", "Zaiqiu Long"], "journal": "BioMed research international", "year": 2021, "abstract": "Endometrial carcinoma (EC) is the fifth widely occurring malignant neoplasm among women all over the world. However, there is still lacking efficacy indicators for EC's prognosis. Here, we analyzed two databases including an RNA-sequencing-based TCGA dataset and a microarray-based GSE106191. After normalizing the raw data, we identified 114 common genes with upregulation and 308 common genes with downregulation in both the TCGA and GSE106191 databases. Bioinformatics analysis showed that the differently expressed genes in EC were related to the IL17 signaling pathway, PI3K-Akt signaling pathway, and cGMP-PKG signaling pathway. Furthermore, we performed the least absolute shrinkage and selection operator (LASSO) Cox regression analysis and generated a signature featuring 17 prognosis-related genes (MAL2, ANKRD22, METTL7B, IL32, ERFE, OAS1, TRPC1, SRPX, RAPGEF4, PSD3, SIMC1, TRPC6, WFS1, PGR, PAMR1, KCNK6, and FAM189A2) and found that it could predict OS in EC patients. The further analysis showed that OAS1, MAL2, ANKRD22, METTL7B, and IL32 were significantly upregulated in EC samples after comparison with normal samples. However, TRPC1, SRPX, RAPGEF4, PSD3, SIMC1, TRPC6, WFS1, PGR, PAMR1, KCNK6, and FAM189A2 were significantly downregulated in EC samples in comparison with normal samples. And correlation analysis showed that our results showed that the expressions of 17 prognosis-related hub genes were significantly correlated based on Pearson correlation. We here offer a newly genetic biomarker for the prediction of EC patients' prognosis.", "claims": [{"id": "claim-method", "statement": "Then, Software R (version 3.5.1, https://www.r-project.org) and “limma” packages (http://www.bioconductor.org/) [16] were applied to select the DEGs existing in the EC samples and control samples.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34337010", "locator": "sections[4] \"2.2. Identification of Differentially Expressed Genes (DEGs)\" paragraph 2", "quote": "Then, Software R (version 3.5.1, https://www.r-project.org) and “limma” packages (http://www.bioconductor.org/) [16] were applied to select the DEGs existing in the EC samples and control samples."}], "state": "complete"}, {"id": "claim-data-access", "statement": "All the data and material were presented in GSE106191 and TCGA (https://portal.gdc.cancer.gov/).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34337010", "locator": "sections[17] \"Data Availability\" paragraph 2", "quote": "All the data and material were presented in GSE106191 and TCGA (https://portal.gdc.cancer.gov/)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34337010"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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