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pmid:34337051
Single-Cell Transcriptomics-Based Study of Transcriptional Regulatory Features in the Mouse Brain Vasculature.
Wei-Wei Lin · Lin-Tao Xu · Yi-Sheng Chen · Ken Go · Chenyu Sun · Yong-Jian Zhu
BioMed research international · 2021 doi:10.1155/2021/7643209
Paper
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Experiment
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Data
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Analysis
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Execution
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Complete file
{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34337051", "kind": "science-record", "revision": 8, "content_hash": "sha256:e9e80c88b638a8c210394dd1a31c2c2a6a2716ebd89593152205bf3502b0b4ff", "created_at": "2026-11-01T15:41:46Z", "updated_at": "2026-11-01T22:47:15Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Single-Cell Transcriptomics-Based Study of Transcriptional Regulatory Features in the Mouse Brain Vasculature.", "doi": "10.1155/2021/7643209", "pmid": "34337051", "authors": ["Wei-Wei Lin", "Lin-Tao Xu", "Yi-Sheng Chen", "Ken Go", "Chenyu Sun", "Yong-Jian Zhu"], "journal": "BioMed research international", "year": 2021, "abstract": "BACKGROUND: The critical role of vascular health on brain function has received much attention in recent years. At the single-cell level, studies on the developmental processes of cerebral vascular growth are still relatively few. Techniques for constructing gene regulatory networks (GRNs) based on single-cell transcriptome expression data have made significant progress in recent years. Herein, we constructed a single-cell transcriptional regulatory network of mouse cerebrovascular cells.\nMETHODS: The single-cell RNA-seq dataset of mouse brain vessels was downloaded from GEO (GSE98816). This cell clustering was annotated separately using singleR and CellMarker. We then used a modified version of the SCENIC method to construct GRNs. Next, we used a mouse version of SEEK to assess whether genes in the regulon were coexpressed. Finally, regulatory module analysis was performed to complete the cell type relationship quantification.\nRESULTS: Single-cell RNA-seq data were used to analyze the heterogeneity of mouse cerebrovascular cells, whereby four cell types including endothelial cells, fibroblasts, microglia, and oligodendrocytes were defined. These subpopulations of cells and marker genes together characterize the molecular profile of mouse cerebrovascular cells. Through these signatures, key transcriptional regulators that maintain cell identity were identified. Our findings identified genes like Lmo2, which play an important role in endothelial cells. The same cell type, for instance, fibroblasts, was found to have different regulatory networks, which may influence the functional characteristics of local tissues.\nCONCLUSIONS: In this study, a transcriptional regulatory network based on single-cell analysis was constructed. Additionally, the study identified and profiled mouse cerebrovascular cells using single-cell transcriptome data as well as defined TFs that affect the regulatory network of the mouse brain vasculature.", "claims": [{"id": "claim-method", "statement": "To test the validity of the above analysis, we applied SEEK analysis to determine GEO datasets with significant coexpression of the regulatory gene Cebpa (Figure 3(d); Fisher's exact test, p = 0.197).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34337051", "locator": "sections[11] \"3.3. 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"GSM2613233", "GSM2613234", "GSM2613235", "GSM2613236", "GSM2613237", "GSM2613238", "GSM2613239", "GSM2613240", "GSM2613241", "GSM2613242", "GSM2613243", "GSM2613244", "GSM2613245", "GSM2613246", "GSM2613247", "GSM2613248", "GSM2613249", "GSM2613250", "GSM2613251", "GSM2613252", "GSM2613253", "GSM2613254", "GSM2613255", "GSM2613256", "GSM2613257", "GSM2613258", "GSM2613259", "GSM2613260", "GSM2613261", "GSM2613262", "GSM2613263", "GSM2613264", "GSM2613265", "GSM2613266", "GSM2613267", "GSM2613268", "GSM2613269", "GSM2613270", "GSM2613271", "GSM2613272", "GSM2613273", "GSM2613274", "GSM2613275", "GSM2613276", "GSM2613277", "GSM2613278", "GSM2613279", "GSM2613280", "GSM2613281", "GSM2613282", "GSM2613283", "GSM2613284", "GSM2613285", "GSM2613286", "GSM2613287", "GSM2613288", "GSM2613289", "GSM2613290", "GSM2613291", "GSM2613292", "GSM2613293", "GSM2613294", "GSM2613295", "GSM2613296", "GSM2613297", "GSM2613298", "GSM2613299", "GSM2613300", "GSM2613301", "GSM2613302", "GSM2613303", "GSM2613304", "GSM2613305", "GSM2613306", "GSM2613307", "GSM2613308", "GSM2613309", "GSM2613310", "GSM2613311", "GSM2613312", "GSM2613313", "GSM2613314", "GSM2613315", "GSM2613316", "GSM2613317", "GSM2613318", "GSM2613319", "GSM2613320", "GSM2613321", "GSM2613322", "GSM2613323", "GSM2613324", "GSM2613325", "GSM2613326", "GSM2613327", "GSM2613328", "GSM2613329", "GSM2613330", "GSM2613331", "GSM2613332", "GSM2613333", "GSM2613334", "GSM2613335", "GSM2613336", "GSM2613337", "GSM2613338", "GSM2613339", "GSM2613340", "GSM2613341", "GSM2613342", "GSM2613343", "GSM2613344", "GSM2613345", "GSM2613346", "GSM2613347", "GSM2613348", "GSM2613349", "GSM2613350", "GSM2613351", "GSM2613352", "GSM2613353", "GSM2613354", "GSM2613355", "GSM2613356", "GSM2613357", "GSM2613358", "GSM2613359", "GSM2613360", "GSM2613361", "GSM2613362", "GSM2613363", "GSM2613364", "GSM2613365", "GSM2613366", "GSM2613367", "GSM2613368", "GSM2613369", "GSM2613370", "GSM2613371", "GSM2613372", "GSM2613373", "GSM2613374", "GSM2613375", "GSM2613376", "GSM2613377", "GSM2613378", "GSM2613379", "GSM2613380", "GSM2613381", "GSM2613382", "GSM2613383", "GSM2613384", "GSM2613385", "GSM2613386", "GSM2613387", "GSM2613388", "GSM2613389", "GSM2613390", "GSM2613391", "GSM2613392", "GSM2613393", "GSM2613394", "GSM2613395", "GSM2613396", "GSM2613397", "GSM2613398", "GSM2613399", "GSM2613400", "GSM2613401", "GSM2613402", "GSM2613403", "GSM2613404", "GSM2613405", "GSM2613406", "GSM2613407", "GSM2613408", "GSM2613409", "GSM2613410", "GSM2613411", "GSM2613412", "GSM2613413", "GSM2613414", "GSM2613415", "GSM2613416", "GSM2613417", "GSM2613418", "GSM2613419", "GSM2613420", "GSM2613421", "GSM2613422", "GSM2613423", "GSM2613424", "GSM2613425", "GSM2613426", "GSM2613427", "GSM2613428", "GSM2613429", "GSM2613430", "GSM2613431", "GSM2613432", "GSM2613433", "GSM2613434", "GSM2613435", "GSM2613436", "GSM2613437", "GSM2613438", "GSM2613439", "GSM2613440", "GSM2613441", "GSM2613442", "GSM2613443", "GSM2613444", "GSM2613445", "GSM2613446", "GSM2613447", "GSM2613448", "GSM2613449", "GSM2613450", "GSM2613451", "GSM2613452", "GSM2613453", "GSM2613454", "GSM2613455", "GSM2613456", "GSM2613457", "GSM2613458", "GSM2613459", "GSM2613460", "GSM2613461", "GSM2613462", "GSM2613463", "GSM2613464", "GSM2613465", "GSM2613466", "GSM2613467", "GSM2613468", "GSM2613469", "GSM2613470", "GSM2613471", "GSM2613472", "GSM2613473", "GSM2613474", "GSM2613475", "GSM2613476", "GSM2613477", "GSM2613478", "GSM2613479", "GSM2613480", "GSM2613481", "GSM2613482", "GSM2613483", "GSM2613484", "GSM2613485", "GSM2613486", "GSM2613487", "GSM2613488", "GSM2613489", "GSM2613490", "GSM2613491", "GSM2613492", "GSM2613493", "GSM2613494", "GSM2613495", "GSM2613496", "GSM2613497", "GSM2613498", "GSM2613499", "GSM2613500", "GSM2613501", "GSM2613502", "GSM2613503", "GSM2613504", "GSM2613505", "GSM2613506", "GSM2613507", "GSM2613508", "GSM2613509", "GSM2613510", "GSM2613511", "GSM2613512", "GSM2613513", "GSM2613514", "GSM2613515", "GSM2613516", "GSM2613517", "GSM2613518", "GSM2613519", "GSM2613520", "GSM2613521", "GSM2613522", "GSM2613523", "GSM2613524", "GSM2613525", "GSM2613526", "GSM2613527", "GSM2613528", "GSM2613529", "GSM2613530", "GSM2613531", "GSM2613532", "GSM2613533", "GSM2613534", "GSM2613535", "GSM2613536", "GSM2613537", "GSM2613538", "GSM2613539", "GSM2613540", "GSM2613541", "GSM2613542", "GSM2613543", "GSM2613544", "GSM2613545", "GSM2613546", "GSM2613547", "GSM2613548", "GSM2613549", "GSM2613550", "GSM2613551", "GSM2613552", "GSM2613553", "GSM2613554", "GSM2613555", "GSM2613556", "GSM2613557", "GSM2613558", "GSM2613559", "GSM2613560", "GSM2613561", "GSM2613562", "GSM2613563", "GSM2613564", "GSM2613565", "GSM2613566", "GSM2613567", "GSM2613568", "GSM2613569", "GSM2613570", "GSM2613571", "GSM2613572", "GSM2613573", "GSM2613574", "GSM2613575", "GSM2613576", "GSM2613577", "GSM2613578", "GSM2613579", "GSM2613580", "GSM2613581", "GSM2613582", "GSM2613583", "GSM2613584", "GSM2613585", "GSM2613586", "GSM2613587", "GSM2613588", "GSM2613589", "GSM2613590", "GSM2613591", "GSM2613592", "GSM2613593", "GSM2613594", "GSM2613595", "GSM2613596", "GSM2613597", "GSM2613598", "GSM2613599", "GSM2613600", "GSM2613601", "GSM2613602", "GSM2613603", "GSM2613604", "GSM2613605", "GSM2613606", "GSM2613607", "GSM2613608", "GSM2613609", "GSM2613610", "GSM2613611", "GSM2613612", "GSM2613613", "GSM2613614", "GSM2613615", "GSM2613616", "GSM2613617", "GSM2613618", "GSM2613619", "GSM2613620", "GSM2613621", "GSM2613622", "GSM2613623", "GSM2613624", "GSM2613625", "GSM2613626", "GSM2613627", "GSM2613628", "GSM2613629", "GSM2613630", "GSM2613631", "GSM2613632", "GSM2613633", "GSM2613634", "GSM2613635", "GSM2613636", "GSM2613637", "GSM2613638", "GSM2613639", "GSM2613640", "GSM2613641", "GSM2613642", "GSM2613643", "GSM2613644", "GSM2613645", "GSM2613646", "GSM2613647", "GSM2613648", "GSM2613649", "GSM2613650", "GSM2613651", "GSM2613652", "GSM2613653", "GSM2613654", "GSM2613655", "GSM2613656", "GSM2613657", "GSM2613658", "GSM2613659", "GSM2613660", "GSM2613661", "GSM2613662", "GSM2613663", "GSM2613664", "GSM2613665", "GSM2613666", "GSM2613667", "GSM2613668", "GSM2613669", "GSM2613670", "GSM2613671", "GSM2613672", "GSM2613673", "GSM2613674", "GSM2613675", "GSM2613676", "GSM2613677", "GSM2613678", "GSM2613679", "GSM2613680", "GSM2613681", "GSM2613682", "GSM2613683", "GSM2613684", "GSM2613685", "GSM2613686", "GSM2613687", "GSM2613688", "GSM2613689", "GSM2613690", "GSM2613691", "GSM2613692", "GSM2613693", "GSM2613694", "GSM2613695", "GSM2613696", "GSM2613697", "GSM2613698", "GSM2613699", "GSM2613700", "GSM2613701", "GSM2613702", "GSM2613703", "GSM2613704", "GSM2613705", "GSM2613706", "GSM2613707", "GSM2613708", "GSM2613709", "GSM2613710", "GSM2613711", "GSM2613712", "GSM2613713", "GSM2613714", "GSM2613715", "GSM2613716", "GSM2613717", "GSM2613718", "GSM2613719", "GSM2613720", "GSM2613721", "GSM2613722", "GSM2613723", "GSM2613724", "GSM2613725", "GSM2613726", "GSM2613727", "GSM2613728", "GSM2613729", "GSM2613730", "GSM2613731", "GSM2613732", "GSM2613733", "GSM2613734", "GSM2613735", "GSM2613736", "GSM2613737", "GSM2613738", "GSM2613739", "GSM2613740", "GSM2613741", "GSM2613742", "GSM2613743", "GSM2613744", "GSM2613745", "GSM2613746", "GSM2613747", "GSM2613748", "GSM2613749", "GSM2613750", "GSM2613751", "GSM2613752", "GSM2613753", "GSM2613754", "GSM2613755", "GSM2613756", "GSM2613757", "GSM2613758", "GSM2613759", "GSM2613760", "GSM2613761", "GSM2613762", "GSM2613763", "GSM2613764", "GSM2613765", "GSM2613766", "GSM2613767", "GSM2613768", "GSM2613769", "GSM2613770", "GSM2613771", "GSM2613772", "GSM2613773", "GSM2613774", "GSM2613775", "GSM2613776", "GSM2613777", "GSM2613778", "GSM2613779", "GSM2613780", "GSM2613781", "GSM2613782", "GSM2613783", "GSM2613784", "GSM2613785", "GSM2613786", "GSM2613787", "GSM2613788", "GSM2613789", "GSM2613790", "GSM2613791", "GSM2613792", "GSM2613793", "GSM2613794", "GSM2613795", "GSM2613796", "GSM2613797", "GSM2613798", "GSM2613799", "GSM2613800", "GSM2613801", "GSM2613802", "GSM2613803", "GSM2613804", "GSM2613805", "GSM2613806", "GSM2613807", "GSM2613808", "GSM2613809", "GSM2613810", "GSM2613811", "GSM2613812", "GSM2613813", "GSM2613814", "GSM2613815", "GSM2613816", "GSM2613817", "GSM2613818", "GSM2613819", "GSM2613820", "GSM2613821", "GSM2613822", "GSM2613823", "GSM2613824", "GSM2613825", "GSM2613826", "GSM2613827", "GSM2613828", "GSM2613829", "GSM2613830", "GSM2613831", "GSM2613832", "GSM2613833", "GSM2613834", "GSM2613835", "GSM2613836", "GSM2613837", "GSM2613838", "GSM2613839", "GSM2613840", "GSM2613841", "GSM2613842", "GSM2613843", "GSM2613844", "GSM2613845", "GSM2613846", "GSM2613847", "GSM2613848", "GSM2613849", "GSM2613850", "GSM2613851", "GSM2613852", "GSM2613853", "GSM2613854", "GSM2613855", "GSM2613856", "GSM2613857", "GSM2613858", "GSM2613859", "GSM2613860", "GSM2613861", "GSM2613862", "GSM2613863", "GSM2613864", "GSM2613865", "GSM2613866", "GSM2613867", "GSM2613868", "GSM2613869", "GSM2613870", "GSM2613871", "GSM2613872", "GSM2613873", "GSM2613874", "GSM2613875", "GSM2613876", "GSM2613877", "GSM2613878", "GSM2613879", "GSM2613880", "GSM2613881", "GSM2613882", "GSM2613883", "GSM2613884", "GSM2613885", "GSM2613886", "GSM2613887", "GSM2613888", "GSM2613889", "GSM2613890", "GSM2613891", "GSM2613892", "GSM2613893", "GSM2613894", "GSM2613895", "GSM2613896", "GSM2613897", "GSM2613898", "GSM2613899", "GSM2613900", "GSM2613901", "GSM2613902", "GSM2613903", "GSM2613904", "GSM2613905", "GSM2613906", "GSM2613907", "GSM2613908", "GSM2613909", "GSM2613910", "GSM2613911", "GSM2613912", "GSM2613913", "GSM2613914", "GSM2613915", "GSM2613916", "GSM2613917", "GSM2613918", "GSM2613919", "GSM2613920", "GSM2613921", "GSM2613922", "GSM2613923", "GSM2613924", "GSM2613925", "GSM2613926", "GSM2613927", "GSM2613928", "GSM2613929", "GSM2613930", "GSM2613931", "GSM2613932", "GSM2613933", "GSM2613934", "GSM2613935", "GSM2613936", "GSM2613937", "GSM2613938", "GSM2613939", "GSM2613940", "GSM2613941", "GSM2613942", "GSM2613943", "GSM2613944", "GSM2613945", "GSM2613946", "GSM2613947", "GSM2613948", "GSM2613949", "GSM2613950", "GSM2613951", "GSM2613952", "GSM2613953", "GSM2613954", "GSM2613955", "GSM2613956", "GSM2613957", "GSM2613958", "GSM2613959", "GSM2613960", "GSM2613961", "GSM2613962", "GSM2613963", "GSM2613964", "GSM2613965", "GSM2613966", "GSM2613967", "GSM2613968", "GSM2613969", "GSM2613970", "GSM2613971", "GSM2613972", "GSM2613973", "GSM2613974", "GSM2613975", "GSM2613976", "GSM2613977", "GSM2613978", "GSM2613979", "GSM2613980", "GSM2613981", "GSM2613982", "GSM2613983", "GSM2613984", "GSM2613985", "GSM2613986", "GSM2613987", "GSM2613988", "GSM2613989", "GSM2613990", "GSM2613991", "GSM2613992", "GSM2613993", "GSM2613994", "GSM2613995", "GSM2613996", "GSM2613997", "GSM2613998", "GSM2613999", "GSM2614000", "GSM2614001", "GSM2614002", "GSM2614003", "GSM2614004", "GSM2614005", "GSM2614006", "GSM2614007", "GSM2614008", "GSM2614009", "GSM2614010", "GSM2614011", "GSM2614012", "GSM2614013", "GSM2614014", "GSM2614015", "GSM2614016", "GSM2614017", "GSM2614018", "GSM2614019", "GSM2614020", "GSM2614021", "GSM2614022", "GSM2614023", "GSM2614024", "GSM2614025", "GSM2614026", "GSM2614027", "GSM2614028", "GSM2614029", "GSM2614030", "GSM2614031", "GSM2614032", "GSM2614033", "GSM2614034", "GSM2614035", "GSM2614036", "GSM2614037", "GSM2614038", "GSM2614039", "GSM2614040", "GSM2614041", "GSM2614042", "GSM2614043", "GSM2614044", "GSM2614045", "GSM2614046", "GSM2614047", "GSM2614048", "GSM2614049", "GSM2614050", "GSM2614051", "GSM2614052", "GSM2614053", "GSM2614054", "GSM2614055", "GSM2614056", "GSM2614057", "GSM2614058", "GSM2614059", "GSM2614060", "GSM2614061", "GSM2614062", "GSM2614063", "GSM2614064", "GSM2614065", "GSM2614066", "GSM2614067", "GSM2614068", "GSM2614069", "GSM2614070", "GSM2614071", "GSM2614072", "GSM2614073", "GSM2614074", "GSM2614075", "GSM2614076", "GSM2614077", "GSM2614078", "GSM2614079", "GSM2614080", "GSM2614081", "GSM2614082", "GSM2614083", "GSM2614084", "GSM2614085", "GSM2614086", "GSM2614087", "GSM2614088", "GSM2614089", "GSM2614090", "GSM2614091", "GSM2614092", "GSM2614093", "GSM2614094", "GSM2614095", "GSM2614096", "GSM2614097", "GSM2614098", "GSM2614099", "GSM2614100", "GSM2614101", "GSM2614102", "GSM2614103", "GSM2614104", "GSM2614105", "GSM2614106", "GSM2614107", "GSM2614108", "GSM2614109", "GSM2614110", "GSM2614111", "GSM2614112", "GSM2614113", "GSM2614114", "GSM2614115", "GSM2614116", "GSM2614117", "GSM2614118", "GSM2614119", "GSM2614120", "GSM2614121", "GSM2614122", "GSM2614123", "GSM2614124", "GSM2614125", "GSM2614126", "GSM2614127", "GSM2614128", "GSM2614129", "GSM2614130", "GSM2614131", "GSM2614132", "GSM2614133", "GSM2614134", "GSM2614135", "GSM2614136", "GSM2614137", "GSM2614138", "GSM2614139", "GSM2614140", "GSM2614141", "GSM2614142", "GSM2614143", "GSM2614144", "GSM2614145", "GSM2614146", "GSM2614147", "GSM2614148", "GSM2614149", "GSM2614150", "GSM2614151", "GSM2614152", "GSM2614153", "GSM2614154", "GSM2614155", "GSM2614156", "GSM2614157", "GSM2614158", "GSM2614159", "GSM2614160", "GSM2614161", "GSM2614162", "GSM2614163", "GSM2614164", "GSM2614165", "GSM2614166", "GSM2614167", "GSM2614168", "GSM2614169", "GSM2614170", "GSM2614171", "GSM2614172", "GSM2614173", "GSM2614174", "GSM2614175", "GSM2614176", "GSM2614177", "GSM2614178", "GSM2614179", "GSM2614180", "GSM2614181", "GSM2614182", "GSM2614183", "GSM2614184", "GSM2614185", "GSM2614186", "GSM2614187", "GSM2614188", "GSM2614189", "GSM2614190", "GSM2614191", "GSM2614192", "GSM2614193", "GSM2614194", "GSM2614195", "GSM2614196", "GSM2614197", "GSM2614198", "GSM2614199", "GSM2614200", "GSM2614201", "GSM2614202", "GSM2614203", "GSM2614204", "GSM2614205", "GSM2614206", "GSM2614207", "GSM2614208", "GSM2614209", "GSM2614210", "GSM2614211", "GSM2614212", "GSM2614213", "GSM2614214", "GSM2614215", "GSM2614216", "GSM2614217", "GSM2614218", "GSM2614219", "GSM2614220", "GSM2614221", "GSM2614222", "GSM2614223", "GSM2614224", "GSM2614225", "GSM2614226", "GSM2614227", "GSM2614228", "GSM2614229", "GSM2614230", "GSM2614231", "GSM2614232", "GSM2614233", "GSM2614234", "GSM2614235", "GSM2614236", "GSM2614237", "GSM2614238", "GSM2614239", "GSM2614240", "GSM2614241", "GSM2614242", "GSM2614243", "GSM2614244", "GSM2614245", "GSM2614246", "GSM2614247", "GSM2614248", "GSM2614249", "GSM2614250", "GSM2614251", "GSM2614252", "GSM2614253", "GSM2614254", "GSM2614255", "GSM2614256", "GSM2614257", "GSM2614258", "GSM2614259", "GSM2614260", "GSM2614261", "GSM2614262", "GSM2614263", "GSM2614264", "GSM2614265", "GSM2614266", "GSM2614267", "GSM2614268", "GSM2614269", "GSM2614270", "GSM2614271", "GSM2614272", "GSM2614273", "GSM2614274", "GSM2614275", "GSM2614276", "GSM2614277", "GSM2614278", "GSM2614279", "GSM2614280", "GSM2614281", "GSM2614282", "GSM2614283", "GSM2614284", "GSM2614285", "GSM2614286", "GSM2614287", "GSM2614288", "GSM2614289", "GSM2614290", "GSM2614291", "GSM2614292", "GSM2614293", "GSM2614294", "GSM2614295", "GSM2614296", "GSM2614297", "GSM2614298", "GSM2614299", "GSM2614300", "GSM2614301", "GSM2614302", "GSM2614303", "GSM2614304", "GSM2614305", "GSM2614306", "GSM2614307", "GSM2614308", "GSM2614309", "GSM2614310", "GSM2614311", "GSM2614312", "GSM2614313", "GSM2614314", "GSM2614315", "GSM2614316", "GSM2614317", "GSM2614318", "GSM2614319", "GSM2614320", "GSM2614321", "GSM2614322", "GSM2614323", "GSM2614324", "GSM2614325", "GSM2614326", "GSM2614327", "GSM2614328", "GSM2614329", "GSM2614330", "GSM2614331", "GSM2614332", "GSM2614333", "GSM2614334", "GSM2614335", "GSM2614336", "GSM2614337", "GSM2614338", "GSM2614339", "GSM2614340", "GSM2614341", "GSM2614342", "GSM2614343", "GSM2614344", "GSM2614345", "GSM2614346", "GSM2614347", "GSM2614348", "GSM2614349", "GSM2614350", "GSM2614351", "GSM2614352", "GSM2614353", "GSM2614354", "GSM2614355", "GSM2614356", "GSM2614357", "GSM2614358", "GSM2614359", "GSM2614360", "GSM2614361", "GSM2614362", "GSM2614363", "GSM2614364", "GSM2614365", "GSM2614366", "GSM2614367", "GSM2614368", "GSM2614369", "GSM2614370", "GSM2614371", "GSM2614372", "GSM2614373", "GSM2614374", "GSM2614375", "GSM2614376", "GSM2614377", "GSM2614378", "GSM2614379", "GSM2614380", "GSM2614381", "GSM2614382", "GSM2614383", "GSM2614384", "GSM2614385", "GSM2614386", "GSM2614387", "GSM2614388", "GSM2614389", "GSM2614390", "GSM2614391", "GSM2614392", "GSM2614393", "GSM2614394", "GSM2614395", "GSM2614396", "GSM2614397", "GSM2614398", "GSM2614399", "GSM2614400", "GSM2614401", "GSM2614402", "GSM2614403", "GSM2614404", "GSM2614405", "GSM2614406", "GSM2614407", "GSM2614408", "GSM2614409", "GSM2614410", "GSM2614411", "GSM2614412", "GSM2614413", "GSM2614414", "GSM2614415", "GSM2614416", "GSM2614417", "GSM2614418", "GSM2614419", "GSM2614420", "GSM2614421", "GSM2614422", "GSM2614423", "GSM2614424", "GSM2614425", "GSM2614426", "GSM2614427", "GSM2614428", "GSM2614429", "GSM2614430", "GSM2614431", "GSM2614432", "GSM2614433", "GSM2614434", "GSM2614435", "GSM2614436", "GSM2614437", "GSM2614438", "GSM2614439", "GSM2614440", "GSM2614441", "GSM2614442", "GSM2614443", "GSM2614444", "GSM2614445", "GSM2614446", "GSM2614447", "GSM2614448", "GSM2614449", "GSM2614450", "GSM2614451", "GSM2614452", "GSM2614453", "GSM2614454", "GSM2614455", "GSM2614456", "GSM2614457", "GSM2614458", "GSM2614459", "GSM2614460", "GSM2614461", "GSM2614462", "GSM2614463", "GSM2614464", "GSM2614465", "GSM2614466", "GSM2614467", "GSM2614468", "GSM2614469", "GSM2614470", "GSM2614471", "GSM2614472", "GSM2614473", "GSM2614474", "GSM2614475", "GSM2614476", "GSM2614477", "GSM2614478", "GSM2614479", "GSM2614480", "GSM2614481", "GSM2614482", "GSM2614483", "GSM2614484", "GSM2614485", "GSM2614486", "GSM2614487", "GSM2614488", "GSM2614489", "GSM2614490", "GSM2614491", "GSM2614492", "GSM2614493", "GSM2614494", "GSM2614495", "GSM2614496", "GSM2614497", "GSM2614498", "GSM2614499", "GSM2614500", "GSM2614501", "GSM2614502", "GSM2614503", "GSM2614504", "GSM2614505", "GSM2614506", "GSM2614507", "GSM2614508", "GSM2614509", "GSM2614510", "GSM2614511", "GSM2614512", "GSM2614513", "GSM2614514", "GSM2614515", "GSM2614516", "GSM2614517", "GSM2614518", "GSM2614519", "GSM2614520", "GSM2614521", "GSM2614522", "GSM2614523", "GSM2614524", "GSM2614525", "GSM2614526", "GSM2614527", "GSM2614528", "GSM2614529", "GSM2614530", "GSM2614531", "GSM2614532", "GSM2614533", "GSM2614534", "GSM2614535", "GSM2614536", "GSM2614537", "GSM2614538", "GSM2614539", "GSM2614540", "GSM2614541", "GSM2614542", "GSM2614543", "GSM2614544", "GSM2614545", "GSM2614546", "GSM2614547", "GSM2614548", "GSM2614549", "GSM2614550", "GSM2614551", "GSM2614552", "GSM2614553", "GSM2614554", "GSM2614555", "GSM2614556", "GSM2614557", "GSM2614558", "GSM2614559", "GSM2614560", "GSM2614561", "GSM2614562", "GSM2614563", "GSM2614564", "GSM2614565", "GSM2614566", "GSM2614567", "GSM2614568", "GSM2614569", "GSM2614570", "GSM2614571", "GSM2614572", "GSM2614573", "GSM2614574", "GSM2614575", "GSM2614576", "GSM2614577", "GSM2614578", "GSM2614579", "GSM2614580", "GSM2614581", "GSM2614582", "GSM2614583", "GSM2614584", "GSM2614585", "GSM2614586", "GSM2614587", "GSM2614588", "GSM2614589", "GSM2614590", "GSM2614591", "GSM2614592", "GSM2614593", "GSM2614594", "GSM2614595", "GSM2614596", "GSM2614597", "GSM2614598", "GSM2614599", "GSM2614600", "GSM2614601", "GSM2614602", "GSM2614603", "GSM2614604", "GSM2614605", "GSM2614606", "GSM2614607", "GSM2614608", "GSM2614609", "GSM2614610", "GSM2614611", "GSM2614612", "GSM2614613", "GSM2614614", "GSM2614615", "GSM2614616", "GSM2614617", "GSM2614618", "GSM2614619", "GSM2614620", "GSM2614621", "GSM2614622", "GSM2614623", "GSM2614624", "GSM2614625", "GSM2614626", "GSM2614627", "GSM2614628", "GSM2614629", "GSM2614630", "GSM2614631", "GSM2614632", "GSM2614633", "GSM2614634", "GSM2614635", "GSM2614636", "GSM2614637", "GSM2614638", "GSM2614639", "GSM2614640", "GSM2614641", "GSM2614642", "GSM2614643", "GSM2614644", "GSM2614645", "GSM2614646", "GSM2614647", "GSM2614648", "GSM2614649", "GSM2614650", "GSM2614651", "GSM2614652", "GSM2614653", "GSM2614654", "GSM2614655", "GSM2614656", "GSM2614657", "GSM2614658", "GSM2614659", "GSM2614660", "GSM2614661", "GSM2614662", "GSM2614663", "GSM2614664", "GSM2614665", "GSM2614666", "GSM2614667", "GSM2614668", "GSM2614669", "GSM2614670", "GSM2614671", "GSM2614672", "GSM2614673", "GSM2614674", "GSM2614675", "GSM2614676", "GSM2614677", "GSM2614678", "GSM2614679", "GSM2614680", "GSM2614681", "GSM2614682", "GSM2614683", "GSM2614684", "GSM2614685", "GSM2614686", "GSM2614687", "GSM2614688", "GSM2614689", "GSM2614690", "GSM2614691", "GSM2614692", "GSM2614693", "GSM2614694", "GSM2614695", "GSM2614696", "GSM2614697", "GSM2614698", "GSM2614699", "GSM2614700", "GSM2614701", "GSM2614702", "GSM2614703", "GSM2614704", "GSM2614705", "GSM2614706", "GSM2614707", "GSM2614708", "GSM2614709", "GSM2614710", "GSM2614711", "GSM2614712", "GSM2614713", "GSM2614714", "GSM2614715", "GSM2614716", "GSM2614717", "GSM2614718", "GSM2614719", "GSM2614720", "GSM2614721", "GSM2614722", "GSM2614723", "GSM2614724", "GSM2614725", "GSM2614726", "GSM2614727", "GSM2614728", "GSM2614729", "GSM2614730", "GSM2614731", "GSM2614732", "GSM2614733", "GSM2614734", "GSM2614735", "GSM2614736", "GSM2614737", "GSM2614738", "GSM2614739", "GSM2614740", "GSM2614741", "GSM2614742", "GSM2614743", "GSM2614744", "GSM2614745", "GSM2614746", "GSM2614747", "GSM2614748", "GSM2614749", "GSM2614750", "GSM2614751", "GSM2614752", "GSM2614753", "GSM2614754", "GSM2614755", "GSM2614756", "GSM2614757", "GSM2614758", "GSM2614759", "GSM2614760", "GSM2614761", "GSM2614762", "GSM2614763", "GSM2614764", "GSM2614765", "GSM2614766", "GSM2614767", "GSM2614768", "GSM2614769", "GSM2614770", "GSM2614771", "GSM2614772", "GSM2614773", "GSM2614774", "GSM2614775", "GSM2614776", "GSM2614777", "GSM2614778", "GSM2614779", "GSM2614780", "GSM2614781", "GSM2614782", "GSM2614783", "GSM2614784", "GSM2614785", "GSM2614786", "GSM2614787", "GSM2614788", "GSM2614789", "GSM2614790", "GSM2614791", "GSM2614792", "GSM2614793", "GSM2614794", "GSM2614795", "GSM2614796", "GSM2614797", "GSM2614798", "GSM2614799", "GSM2614800", "GSM2614801", "GSM2614802", "GSM2614803", "GSM2614804", "GSM2614805", "GSM2614806", "GSM2614807", "GSM2614808", "GSM2614809", "GSM2614810", "GSM2614811", "GSM2614812", "GSM2614813", "GSM2614814", "GSM2614815", "GSM2614816", "GSM2614817", "GSM2614818", "GSM2614819", "GSM2614820", "GSM2614821", "GSM2614822", "GSM2614823", "GSM2614824", "GSM2614825", "GSM2614826", "GSM2614827", "GSM2614828", "GSM2614829", "GSM2614830", "GSM2614831", "GSM2614832", "GSM2614833", "GSM2614834", "GSM2614835", "GSM2614836", "GSM2614837", "GSM2614838", "GSM2614839", "GSM2614840", "GSM2614841", "GSM2614842", "GSM2614843", "GSM2614844", "GSM2614845", "GSM2614846", "GSM2614847", "GSM2614848", "GSM2614849", "GSM2614850", "GSM2614851", "GSM2614852", "GSM2614853", "GSM2614854", "GSM2614855", "GSM2614856", "GSM2614857", "GSM2614858", "GSM2614859", "GSM2614860", "GSM2614861", "GSM2614862", "GSM2614863", "GSM2614864", "GSM2614865", "GSM2614866", "GSM2614867", "GSM2614868", "GSM2614869", "GSM2614870", "GSM2614871", "GSM2614872", "GSM2614873", "GSM2614874", "GSM2614875", "GSM2614876", "GSM2614877", "GSM2614878", "GSM2614879", "GSM2614880", "GSM2614881", "GSM2614882", "GSM2614883", "GSM2614884", "GSM2614885", "GSM2614886", "GSM2614887", "GSM2614888", "GSM2614889", "GSM2614890", "GSM2614891", "GSM2614892", "GSM2614893", "GSM2614894", "GSM2614895", "GSM2614896", "GSM2614897", "GSM2614898", "GSM2614899", "GSM2614900", "GSM2614901", "GSM2614902", "GSM2614903", "GSM2614904", "GSM2614905", "GSM2614906", "GSM2614907", "GSM2614908", "GSM2614909", "GSM2614910", "GSM2614911", "GSM2614912", "GSM2614913", "GSM2614914", "GSM2614915", "GSM2614916", "GSM2614917", "GSM2614918", "GSM2614919", "GSM2614920", "GSM2614921", "GSM2614922", "GSM2614923", "GSM2614924", "GSM2614925", "GSM2614926", "GSM2614927", "GSM2614928", "GSM2614929", "GSM2614930", "GSM2614931", "GSM2614932", "GSM2614933", "GSM2614934", "GSM2614935", "GSM2614936", "GSM2614937", "GSM2614938", "GSM2614939", "GSM2614940", "GSM2614941", "GSM2614942", "GSM2614943", "GSM2614944", "GSM2614945", "GSM2614946", "GSM2614947", "GSM2614948", "GSM2614949", "GSM2614950", "GSM2614951", "GSM2614952", "GSM2614953", "GSM2614954", "GSM2614955", "GSM2614956", "GSM2614957", "GSM2614958", "GSM2614959", "GSM2614960", "GSM2614961", "GSM2614962", "GSM2614963", "GSM2614964", "GSM2614965", "GSM2614966", "GSM2614967", "GSM2614968", "GSM2614969", "GSM2614970", "GSM2614971", "GSM2614972", "GSM2614973", "GSM2614974", "GSM2614975", "GSM2614976", "GSM2614977", "GSM2614978", "GSM2614979", "GSM2614980", "GSM2614981", "GSM2614982", "GSM2614983", "GSM2614984", "GSM2614985", "GSM2614986", "GSM2614987", "GSM2614988", "GSM2614989", "GSM2614990", "GSM2614991", "GSM2614992", "GSM2614993", "GSM2614994", "GSM2614995", "GSM2614996", "GSM2614997", "GSM2614998", "GSM2614999", "GSM2615000", "GSM2615001", "GSM2615002", "GSM2615003", "GSM2615004", "GSM2615005", "GSM2615006", "GSM2615007", "GSM2615008", "GSM2615009", "GSM2615010", "GSM2615011", "GSM2615012", "GSM2615013", "GSM2615014", "GSM2615015", "GSM2615016", "GSM2615017", "GSM2615018", "GSM2615019", "GSM2615020", "GSM2615021", "GSM2615022", "GSM2615023", "GSM2615024", "GSM2615025", "GSM2615026", "GSM2615027", "GSM2615028", "GSM2615029", "GSM2615030", "GSM2615031", "GSM2615032", "GSM2615033", "GSM2615034", "GSM2615035", "GSM2615036", "GSM2615037", "GSM2615038", "GSM2615039", "GSM2615040", "GSM2615041", "GSM2615042", "GSM2615043", "GSM2615044", "GSM2615045", "GSM2615046", "GSM2615047", "GSM2615048", "GSM2615049", "GSM2615050", "GSM2615051", "GSM2615052", "GSM2615053", "GSM2615054", "GSM2615055", "GSM2615056", "GSM2615057", "GSM2615058", "GSM2615059", "GSM2615060", "GSM2615061", "GSM2615062", "GSM2615063", "GSM2615064", "GSM2615065", "GSM2615066", "GSM2615067", "GSM2615068", "GSM2615069", "GSM2615070", "GSM2615071", "GSM2615072", "GSM2615073", "GSM2615074", "GSM2615075", "GSM2615076", "GSM2615077", "GSM2615078", 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"GSM2615718", "GSM2615719", "GSM2615720", "GSM2615721", "GSM2615722", "GSM2615723", "GSM2615724"], "sample_count": 3186, "group_sizes": null, "group_sizes_basis": null, "platform": "13112", "taxon": "Mus musculus", "title": "Single cell RNA-seq of mouse brain vascular transcriptomes", "resolution_checked_at": "2026-11-01T15:39:55Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}], "gaps": ["asset:data:geo:GSE98816 not downloaded", "asset:data:geo:GSE98816 licence not read"]}, "analysis": {"state": "partial", "methods": [{"id": "method-1", "family": "contingency_analysis", "specific": "fisher_exact", "label": "Categorical (contingency table) analysis", "dsl_operations": ["contingency_table", "chi_square_test"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["categorical_cross_tabulation"], "evidence": {"score": 1.0, "band": "verbatim", "quote": "To test the validity of the above analysis, we applied SEEK analysis to determine GEO datasets with significant coexpression of the regulatory gene Cebpa (Figure 3(d); Fisher's exact test, p = 0.197)."}}, {"id": "method-2", "family": "network_analysis", "specific": "ppi_network", "label": "Network / pathway analysis", "dsl_operations": ["build_graph", "centrality_scores", "pathway_enrich"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["edge_list"], "evidence": {"score": 0.8, "band": "high", "quote": "protein-protein interaction"}}], "targets": [], "gaps": ["method.reader_differs_from_extractor"]}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", 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METHODS: The single-cell RNA-seq dataset of mouse brain vessels was downloade", "ionship quantification. RESULTS: Single-cell RNA-seq data were used to analyze the heterogeneity"], "order_basis": "paper_text_order:0", "id": "assay:1"}, {"role": "data_generation", "definition_id": "scrna_seq", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "unsupported", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["Single-Cell Transcriptomics-Based Study of Transcriptional Regulatory Fe", "of mouse cerebrovascular cells. METHODS: The single-cell RNA-seq dataset of mouse brain vessels was downloade", "l type relationship quantification. RESULTS: Single-cell RNA-seq data were used to analyze the heterogeneity"], "order_basis": "paper_text_order:0", "id": "assay:2"}, {"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["Study of Transcriptional Regulatory Features in the Mouse Brain Vasculature.", "f Transcriptional Regulatory Features in the Mouse Brain Vasculature.", "e-cell transcriptional regulatory network of mouse cerebrovascular cells. METHODS: The single-c", "METHODS: The single-cell RNA-seq dataset of mouse brain vessels was downloaded from GEO (GSE98"], "order_basis": "paper_text_order:34", "id": "assay:3"}, {"role": "analysis", "definition_id": "contingency_analysis", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["contingency_table", "chi_square_test"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["To test the validity of the above analysis, we applied SEEK analysis to determine GEO datasets with significant coexpression of the regulatory gene Cebpa (Figure 3(d); Fisher's exact test, p = 0.197)."], "order_basis": "consumes_earlier_output", "id": "assay:4"}]}