Identification of Hub Genes Associated with the Pathogenesis of Intracranial Aneurysm via Integrated Bioinformatics Analysis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34354366", "kind": "science-record", "revision": 8, "content_hash": "sha256:69fd4bcd79f8b1566b0a24a882bc8620072a8d6ed5490792c5b7b2f8b048449a", "created_at": "2026-11-01T15:41:47Z", "updated_at": "2026-11-01T22:47:16Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identification of Hub Genes Associated with the Pathogenesis of Intracranial Aneurysm via Integrated Bioinformatics Analysis.", "doi": "10.2147/IJGM.S320396", "pmid": "34354366", "authors": ["Aifang Zhong", "Ning Ding", "Yang Zhou", "Guifang Yang", "Zhenyu Peng", "Hongliang Zhang", "Xiangping Chai"], "journal": "International journal of general medicine", "year": 2021, "abstract": "BACKGROUND: At present, the pathogenesis of intracranial aneurysms (IA) remains unclear, which significantly hinders the development of novel strategies for the clinical treatment. In this study, bioinformatics methods were used to identify the potential hub genes and pathways associated with the pathogenesis of IA.\nMETHODS: The gene expression datasets of patients with intracranial aneurysm were downloaded from the Gene Expression Database (GEO), and the different data sets were integrated by the robust rank aggregation (RRA) method to identify the differentially expressed genes between patients with intracranial aneurysm and the controls. The functional enrichment analyses of the significant differentially expressed genes (DEGs) were performed and the protein-protein interaction (PPI) network was constructed; thereafter, the hub genes were screened by cytoHubba plug-in of Cytoscape, and finally sequencing dataset GSE122897 was used to verify the hub genes.\nRESULTS: The GSE15629, GSE75436, GSE26969, and GSE6551 expression profiles have been included in this study, including 34 intracranial aneurysm samples and 26 control samples. The four datasets obtained 136 significant DEGs (45 up-regulated, 91 down-regulated). Enrichment analysis showed that the extracellular matrix structural constituent and the ECM-receptor interaction were closely related to the occurrence of IA. It was finally determined that eight hub genes associated with the development of IA, including VCAN, COL1A1, COL11A1, COL5A1, COL5A2, POSTN, THBS2, and CDH2.\nCONCLUSION: The discovery of potential hub genes and pathways could enhance the understanding of the molecular mechanisms associated with the development of IA. 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No dataset was downloaded and no licence was read."}}, "can_run": {"dsl_operations": ["normalise_counts", "differential_expression_fit", "fdr_bh"], "assets": ["source:pmid:34354366", "data:geo:GSE122897", "asset:table:pmid:34354366:1", "asset:table:pmid:34354366:2", "asset:table:pmid:34354366:3"], "steps": ["run_analysis"]}, "must_build": {"dsl_operations": [], "converters": [], "readers": [], "capabilities": [], "data_gaps": ["data:geo:GSE122897"]}, "gaps": []}, "review": {"state": "partial", "machine_critique": {"kind": "extraction_review", "reader": {"model": "MiniMax-M3.1-Flash", "provider": "minimax", "provider_family": "minimax", "read_at": "2026-10-31T18:33:19Z", "independence": "single_reader"}, "evidence_check": {"method": "verbatim", "availability": "verbatim"}, "calls": 3, "usage": {"prompt_tokens": 30618, "completion_tokens": 1739, "cached_tokens": 792}}, "human_critique": null, "stages": [{"id": "extraction", "state": "complete", "reader": "MiniMax-M3.1-Flash", "provider": "minimax"}], "gaps": ["review.independent_reader", "review.machine_critique", "review.human_critique"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "pmid:34354366", "type": "paper", "label": "pmid:34354366"}, {"id": "source:pmid:34354366", "type": "manuscript", "label": "PMID 34354366 full text"}, {"id": "data:geo:GSE122897", "type": "dataset", "label": "GEO GSE122897"}], "activities": [{"id": "activity-reading-sci-2026-11-01", "type": "classification", "used": [{"id": "pmid:34354366", "type": "paper", "label": "pmid:34354366"}, {"id": "source:pmid:34354366", "type": "manuscript", "label": "PMID 34354366 full text"}, {"id": "data:geo:GSE122897", "type": "dataset", "label": "GEO GSE122897"}], "agent": "agent:corpus-assay-engine"}, {"id": "activity-read-34354366", "type": "extraction", "used": ["source:pmid:34354366"], "agent": "agent:minimax-reader"}, {"id": "activity-resolve-GSE122897", "type": "accession_resolution", "used": ["data:geo:GSE122897"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}, {"id": "agent:minimax-reader", "type": "software", "label": "MiniMax literature reader"}], "source_run_id": "reading-sci-2026-11-01", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "pmid:34354366", "parent_hash": "sha256:d2b7cfaf9c12a104b9101612a3a0bb7120fe3231612cdeb41b90c558d8100d43", "fork_of": null, "generation": 7}, "gaps": ["asset:data:geo:GSE122897 licence not read", "asset:data:geo:GSE122897 not downloaded", "asset:data:geo:GSE15629 licence not read", "asset:data:geo:GSE15629 not downloaded", "asset:data:geo:GSE26969 licence not read", "asset:data:geo:GSE26969 not downloaded", "asset:data:geo:GSE6551 licence not read", "asset:data:geo:GSE6551 not downloaded", "asset:data:geo:GSE75436 licence not read", "asset:data:geo:GSE75436 not downloaded", "execution.not_run", "review.human_critique", "review.independent_reader", "review.machine_critique"], "assays": [{"role": "analysis", "definition_id": "differential_expression", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["normalise_counts", "differential_expression_fit", "fdr_bh"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Next, the package of “limma” in R software was used to identify the DEGs."], "order_basis": "analysis_only", "id": "assay:1"}]}