SERPINH1, Targeted by miR-29b, Modulated Proliferation and Migration of Human Retinal Endothelial Cells Under High Glucose Conditions.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34377003", "kind": "science-record", "revision": 8, "content_hash": "sha256:51b79820f0abf4a930d39301a08b850b5ad384184a4abb699c43e611834f599b", "created_at": "2026-11-01T15:41:47Z", "updated_at": "2026-11-01T22:47:18Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "SERPINH1, Targeted by miR-29b, Modulated Proliferation and Migration of Human Retinal Endothelial Cells Under High Glucose Conditions.", "doi": "10.2147/DMSO.S307771", "pmid": "34377003", "authors": ["Lingfei Hu", "Yinping Liu", "Chaobing Wei", "Huixiang Jin", "Lixin Mei", "Changfan Wu"], "journal": "Diabetes, metabolic syndrome and obesity : targets and therapy", "year": 2021, "abstract": "AIM: In the present study, we performed bioinformatics studies and in vitro functional assays to explore the underlying role of serpin family H member 1 (SERPINH1) in the diabetic retinopathy.\nMETHODS: Common differentially expressed genes (DEGs) between diabetic retinal tissues and normal retinal tissues were analyzed using Gene Expression Omnibus (GEO) database. The proliferation and migration of human retinal endothelial cells (HRECs) was evaluated by MTS, EdU and wound healing assays, respectively; the miRNA and mRNAs expression levels of hub genes in HRECs were determined using quantitative real-time PCR (qRT-PCR). Protein levels were determined using a Western blot assay.\nRESULTS: A total of 189 common DEGs were screened between two GEO datasets (GSE60436 and GSE94019), and ten potential hub genes that may link to the progression of diabetic retinopathy were detected. The qRT-PCR results showed that collagen, type I, alpha 1 (COL1A1), Collagen, type I, alpha 2 (COL1A2) and serpin family H member 1 (SERPINH1) mRNA expression levels were up-regulated in the HRECs after being exposed to high glucose for 48 h. Silence of SERPINH1 repressed the high glucose-induced increase in proliferation and migration of HRECs. SERPINH1 was a target of miR-29b and was suppressed by miR-29 in HRECs. SERPINH1 overexpression promoted HREC proliferation and migration. Furthermore, miR-29b suppressed HREC proliferation and migration under high-glucose stimulation, which was significantly attenuated by enforced expression of SERPINH1.\nCONCLUSION: In conclusion, by performing the integrated bioinformatics analysis, the present study suggested that 3 hub genes (COL1A1, COL1A2 and SERPINH1) may be associated with diabetic retinopathy pathophysiology. Further mechanistic studies indicated that miR-29b/SERPINH1 signaling participated in high glucose-induced enhancement in the proliferation and migration of HRECs.", "claims": [{"id": "claim-method", "statement": "Unpaired Student’s t-test or one-way analysis of variance followed by the Bonferroni’s post hoc test was used to assess statistical differences between/among groups in multiple comparisons.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34377003", "locator": "sections[14] \"Statistical Analysis\" paragraph 2", "quote": "Unpaired Student’s t-test or one-way analysis of variance followed by the Bonferroni’s post hoc test was used to assess statistical differences between/among groups in multiple comparisons."}], "state": "complete"}, {"id": "claim-data-access", "statement": "All the data are available.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34377003", "locator": "sections[27] \"Data Sharing Statement\" paragraph 2", "quote": "All the data are available."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34377003"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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