SMOX expression predicts the prognosis of non-small cell lung cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34422960", "kind": "science-record", "revision": 8, "content_hash": "sha256:199ebe1cdf14567c5fe9e6865b2b38284bc79d6abbb2656c6bca5af760620ba5", "created_at": "2026-11-01T15:41:48Z", "updated_at": "2026-11-01T22:04:32Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "SMOX expression predicts the prognosis of non-small cell lung cancer.", "doi": "10.21037/atm-21-998", "pmid": "34422960", "authors": ["Zhanghao Huang", "Shuo Wang", "Hai-Jian Zhang", "You Lang Zhou", "Jia-Hai Shi"], "journal": "Annals of translational medicine", "year": 2021, "abstract": "BACKGROUND: The development of non-small cell lung cancer (NSCLC) is very rapid, and the effect of its treatment is often closely related to the diagnosis time of the disease. Therefore, simple and convenient tumor biomarkers are helpful for the timely diagnosis and prevention of NSCLC.\nMETHODS: Through univariate and multivariate Cox regression analyses, SMOX was determined as an independent prognostic factor of GSE42127, GSE41271, GSE68465, and TCGA datasets. Furthermore, western blot, reverse transcription-polymerase chain reaction (RT-PCR), and immunohistochemical analysis were performed to confirm the predictive efficiency of SMOX expression in NSCLC.\nRESULTS: Patients were divided into high and low expression groups according to the median value of SMOX expression, and Kaplan-Meier curves of multiple datasets indicated that patients with low SMOX expression had a better survival rate. According to the analysis of immune infiltration, the immune microenvironment, and immune checkpoints, SMOX expression of the high and low groups showed differences in immunity in NSCLC. By comparing cancer and adjacent tissues using western blot analysis, RT-PCR and immunohistochemical analysis, we found that SMOX was highly expressed in tumor tissues and had low expression in adjacent tissues. Simultaneously, the Kaplan-Meier curve suggested that among the 155 NSCLC patients, those with low SMOX expression had better survival.\nCONCLUSIONS: SMOX can be used as an effective predictive target for NSCLC.", "claims": [{"id": "claim-method", "statement": "Univariate and multivariate Cox regression analyses were used to calculate 95% confidence interval (CI) and hazard ratio (HR), aiming to determine independent prognostic factors for lung cancer.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34422960", "locator": "sections[4] \"Establishment and verification of independent prognostic factors\" paragraph 2", "quote": "Univariate and multivariate Cox regression analyses were used to calculate 95% confidence interval (CI) and hazard ratio (HR), aiming to determine independent prognostic factors for lung cancer."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Among them, GSE41271 contained 275 tumor samples, GSE42127 contained 176 tumor samples, and GSE68465 contained 443 samples.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34422960", "locator": "sections[3] \"Source and collation of data\" paragraph 2", "quote": "Among them, GSE41271 contained 275 tumor samples, GSE42127 contained 176 tumor samples, and GSE68465 contained 443 samples."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34422960"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE41271"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE41271"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:34422960", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.21037/atm-21-998", "sha256": "sha256:0fc392235b3d85a3e5ef068ed2c4244649b302bdc2e885dd0b32f4e1554e18d2", "bytes": 47351, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.21037/atm-21-998", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC8339854", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE41271", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE41271", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE41271", "version": null, "note": "Among them, GSE41271 contained 275 tumor samples, GSE42127 contained 176 tumor samples, and GSE68465 contained 443 samples.", "structure": 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The physical bench the paper used is not, so a physical validation cannot be run here."}, "data": {"tier": "R1", "reachable": true, "accessions": ["GSE41271", "GSE42127", "GSE68465"], "resolved_accessions": ["GSE41271", "GSE42127", "GSE68465"], "statements": ["Among them, GSE41271 contained 275 tumor samples, GSE42127 contained 176 tumor samples, and GSE68465 contained 443 samples."], "basis": "The paper names GSE41271, GSE42127, GSE68465 in Gene Expression Omnibus (GEO) and the accession resolves in the repository. 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Furthermore, western blot, reverse transcription-polymerase chain reac", "comparing cancer and adjacent tissues using western blot analysis, RT-PCR and immunohistochemical ana", "ther verify the expression of SMOX in NSCLC, western blot analysis was performed. It was found that SM", "External experimental verification. (A,B) 24 Western blot analysis of tumors and adjacent tissues of N"], "order_basis": "paper_text_order:504", "id": "assay:1"}, {"role": "data_generation", "definition_id": "ihc", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["tion-polymerase chain reaction (RT-PCR), and immunohistochemical analysis were performed to confirm the predi", "sues using western blot analysis, RT-PCR and immunohistochemical analysis, we found that SMOX was highly expr", "ined by RT-PCR analysis (Figure 8C) (20,21). Immunohistochemical analysis showed that SMOX was indeed highly", "mor tissues and low in adjacent tissues. (D) Immunohistochemical analysis showed the expression of SMOX in tu"], "order_basis": "paper_text_order:580", "id": "assay:2"}, {"role": "analysis", "definition_id": "survival_analysis", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["cox_ph"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Univariate and multivariate Cox regression analyses were used to calculate 95% confidence interval (CI) and hazard ratio (HR), aiming to determine independent prognostic factors for lung cancer."], "order_basis": "consumes_earlier_output", "id": "assay:3"}]}