Identification of abnormally high expression of POGZ as a new biomarker associated with a poor prognosis in osteosarcoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34474553", "kind": "science-record", "revision": 8, "content_hash": "sha256:395511be718f5cc3f4eb08cd853bab9f9d0bd1f22de69478e1b80d95e3f120af", "created_at": "2026-11-01T15:41:50Z", "updated_at": "2026-11-01T22:04:37Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identification of abnormally high expression of POGZ as a new biomarker associated with a poor prognosis in osteosarcoma.", "doi": "10.4081/ejh.2021.3264", "pmid": "34474553", "authors": ["Sikuan Zheng", "Yue Liu", "Haohe Sun", "Jingyu Jia", "Tianlong Wu", "Rui Ding", "Xigao Cheng"], "journal": "European journal of histochemistry : EJH", "year": 2021, "abstract": "Osteosarcoma (OS) is the most prevalent malignant bone tumor in children and young adults. There is an urgent need for a novel biomarker related to the prognosis of OS. We performed a meta-analysis incorporating six independent datasets and performed a survival analysis with one independent dataset GSE21257 in the GEO database for gene screening. The results revealed that one potential biomarker related to OS survival, POGZ was the most significantly upregulated gene. We also verified that the POGZ was overexpressed in clinical samples. The survival analysis revealed that POGZ is associated with a poor prognosis in OS. Moreover, flow cytometry analysis of isolated OS cells demonstrated that OS cells were arrested in the G1 phase after POGZ knockdown. The RNA-seq results indicated that POGZ was co-expressed with CCNE1 and CCNB1. Pathway analysis showed that genes associated with high expression levels of POGZ were related to the cell cycle pathway. A cell model was constructed to detect the effects of POGZ. After POGZ knockdown, OS cell proliferation, invasion and migration were all decreased. Therefore, POGZ is an important gene for evaluating the prognosis of OS patients and is a potential therapeutic target.", "claims": [{"id": "claim-method", "statement": "Subsequently, the “mama” package was used to group OS patients and normal controls, perform a meta-analysis, and obtain the z-score value.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34474553", "locator": "sections[4] \"Data extraction and analysis of screened genes\" paragraph 2", "quote": "Subsequently, the “mama” package was used to group OS patients and normal controls, perform a meta-analysis, and obtain the z-score value."}], "state": "complete"}, {"id": "claim-data-access", "statement": "After excluding 244 studies involving cell lines and 31 nonconforming studies in the GEO, we selected 6 studies, whose data were included in the GSE11414,15 GSE12865,16 GSE14359,17 GSE16102,18 GSE42352,19,20 GSE4257221 datasets, for inclusion in the meta-analysis (Supplementary Figure 1).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34474553", "locator": "sections[18] \"Identification of genes that are significantly related to the prognosis of OS\" paragraph 2", "quote": "After excluding 244 studies involving cell lines and 31 nonconforming studies in the GEO, we selected 6 studies, whose data were included in the GSE11414,15 GSE12865,16 GSE14359,17 GSE16102,18 GSE42352,19,20 GSE4257221 datasets, for inclusion in the meta-analysis (Supplementary Figure 1)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34474553"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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