Unraveling the relevance of the polyadenylation factor EhCFIm25 in Entamoeba histolytica through proteomic analysis.

{"sci_version": "1.0", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34486252", "kind": "science-record", "revision": 3, "content_hash": "sha256:86282fd3514d4b40e27d8b95e0630da74a439e8af8ddbad5cf993a82d6ef1b27", "created_at": "2026-11-01T15:41:50Z", "updated_at": "2026-11-01T18:56:43Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Unraveling the relevance of the polyadenylation factor EhCFIm25 in Entamoeba histolytica through proteomic analysis.", "doi": "10.1002/2211-5463.13287", "pmid": "34486252", "authors": ["América Itzallana Salgado-Martínez", "Rodolfo Gamaliel Avila-Bonilla", "Esther Ramírez-Moreno", "Carlos Alberto Castañón-Sánchez", "César López-Camarillo", "Laurence A Marchat"], "journal": "FEBS open bio", "year": 2021, "abstract": "We recently reported that silencing of the polyadenylation factor EhCFIm25 in Entamoeba histolytica, the protozoan which causes human amoebiasis, affects trophozoite proliferation, death, and virulence, suggesting that EhCFIm25 may have potential as a new biochemical target. Here, we performed a shotgun proteomic analysis to identify modulated proteins that could explain this phenotype. Data are available via ProteomeXchange with identifier PXD027784. Our results revealed changes in the abundance of 75 proteins. Interestingly, STRING analysis, functional GO-term annotations, KEGG analyses, and literature review showed that modulated proteins are mainly related to glycolysis and carbon metabolism, cytoskeleton dynamics, and parasite virulence, as well as gene expression and protein modifications. 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http://www.proteomexchange.org/, reference number PXD027784.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The data that support the findings of this study are openly available in the ProteomeXchange Consortium at http://www.proteomexchange.org/, reference number PXD027784.", "access_statement_kind": "data_availability_statement", "sample_accessions": [], "sample_count": null, "group_sizes": null, "group_sizes_basis": null, "platform": null, "taxon": null, "title": "A proteomic approach for unravelling the relevance of the polyadenylation factor EhCFIm25 in Entamoeba histolytica survival and virulence", "resolution_checked_at": "2026-10-31T18:35:55Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "asset:table:pmid:34486252:1", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:96d580c6a91a345bb6b64ad1864e1b3cf4cb163b85efd7170ef40805fc4e9b62", "bytes": 7158, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[13]/table-wrap[1]#feb413287-tbl-0001", "version": null, "note": "Modulated proteins in EhCFIm25‐silenced trophozoites", "structure": {"pmcid": "PMC8487052", "section_index": 13, "section_title": "Identification of modulated proteins following EhCFIm25 silencing", "row_count": 70, "column_count": 7}, "table": {"label": "Table 1", "caption": "Modulated proteins in EhCFIm25‐silenced trophozoites", "rows": [["Gene ID", "UniProt", "NCBI", "Protein description", "EhCFIm25‐dsRNA vs nontreated (control) (log2 fold change)", "EhCFIm25‐dsRNA vs gfp‐dsRNA (log2 fold change)", "gfp‐dsRNA vs nontreated (control) (log2 fold change)"], ["Glycolysis and carbon metabolism"], ["Increased"], ["EHI_178960", "C4LUV9", "XP_656290.1", "ACD", "1.571976609", "1.838014699", "0.26603809"], ["Reduced"], ["EHI_000730", "C4LZC2", "XP_653173.1", "PPi_PFK", "−8.953922033", "−6.824743753", "2.129178281"], ["EHI_188180", "C4M192", "XP_653686.1", "PGK", "−3.53422426", "−1.70492415", "1.82930011"], ["EHI_130700", "C4LXE8", "XP_649161.1", "ENO", "−3.664724348", "−2.623408105", "1.041316242"], ["EHI_051060", "C4LTX6", "XP_657019.2", "PFOR", "−5.023063656", "−3.386220638", "1.636843018"], ["EHI_009530", "Q24801", "XP_657332.1", "PK/PPDK", "−3.443328065", "−2.212267448", "1.231060617"], ["EHI_150490", "C4M230", "XP_652300.1", "ADHE", "−3.338295165", "−2.276880037", "1.061415128"], ["EHI_125950", "C4LVY0", "XP_650419.1", "Alcohol dehydrogenase_putative (ADH)", "−2.493758657", "−1.026187504", "1.467571153"], ["EHI_166490", "C4M7F5", "XP_652262.2", "Alcohol dehydrogenase_putative (ADH)", "−1.048881287", "−1.286563624", "−0.237682337"], ["EHI_198620", "C4LWQ8", "XP_655201.2", "PPi‐PEPCK3", "−8.318126762", "−5.902664315", "2.415462446"], ["EHI_044970", "Q9NH04", "XP_648590.1", "ME", "−3.371544637", "−2.282862579", "1.088682059"], ["EHI_014410", "B1N2Y0", "XP_001913546.1", "MDH_ putative", "−2.539964263", "−1.29009284", "1.249871423"], ["EHI_070720", "C4M5S9", "XP_654310.1", "L‐myo‐inositol‐1‐phosphate synthase", "−5.862557542", "−4.718114024", "1.144443518"], ["Not detected"], ["EHI_161010", "B1N3H7", "XP_001913743.1", "Anaerobic G3PDH subunit A_ putative", "‐", "‐", "2.79771936"], ["Cytoskeleton dynamics and parasite virulence"], ["Increased"], ["EHI_110180", "C4LU72", "XP_657028.1", "Myosin heavy chain", "1.742008798", "1.915355481", "0.173346683"], ["EHI_111050", "C4M5I6", "XP_657596.1", "ARP2", "2.48265", "1.660252011", "−0.822397989"], ["EHI_058090", "C4M8B0", "XP_651163.1", "Ras family GTPase", "2.231234072", "1.425210378", "−0.806023694"], ["EHI_196940", "C4LVB2", "XP_653132.1", "Heat‐shock protein 90_ putative", "2.326638075", "1.816849869", "−0.509788206"], ["EHI_133900", "B1N3C7", "XP_001913693.1", "Galactose‐inhibitable lectin 170 kDa subunit_ putative (Gal/GalNAc lectin)", "2.50643645", "1.484150956", "−1.022285494"], ["Reduced"], ["EHI_105210", "C4M126", "XP_653931.1", "F‐BAR domain‐containing protein", "−1.174858759", "−1.873880924", "−0.699022165"], ["EHI_094060", "B1N322", "XP_001913588.1", "Actin‐binding protein_ putative (filamin)", "−3.592858259", "−4.086887697", "−0.494029438"], ["EHI_164430", "C4M1S5", "XP_648375.2", "Actinin‐like protein_ putative (α‐actinin 1)", "−6.424663932", "−5.410549507", "1.014114425"], ["EHI_199000", "C4LWU6", "XP_653283.1", "Calponin homology domain protein_ putative (α‐actinin 2)", "−5.42520244", "−4.937989077", "0.487213363"], ["EHI_136150", "C4M295", "XP_655240.2", "Adenylyl CAP", "−3.26152685", "−3.039265215", "0.222261635"], ["EHI_177990", "C4M553", "XP_651490.1", "Leucine‐rich repeat containing protein", "−9.129552234", "−4.675234233", "4.454318001"], ["EHI_167060", "B1N309", "XP_001913575.1", "Rab GDP dissociation inhibitor", "−2.913843088", "−1.601375505", "1.312467583"], ["EHI_098280", "C4LXB1", "XP_653621.1", "14‐3‐3 protein (EhP2)", "−4.15541176", "−3.696594368", "0.458817392"], ["EHI_006810", "C4M0F4", "XP_654465.1", "14‐3‐3 protein 2 (EhP3)", "−3.628432526", "−4.653662911", "−1.025230385"], ["EHI_159160", "C4LX20", "XP_648827.1", "SOD", "−1.016518349", "−1.037119891", "−0.020601541"], ["EHI_121620", "B1N5Y9", "XP_001914605.1", "Peroxiredoxin_ putative (PRDX)", "−3.343027913", "−2.14245569", "1.200572222"], ["EHI_156710", "B1N502", "XP_001914268.1", "3‐ketoacyl‐CoA synthase 4_ putative", "−7.753320965", "−5.739869952", "2.013451013"], ["EHI_199590", "C4M770", "XP_654737.1", "70 kDa heat‐shock protein_ putative", "−6.306522113", "−6.50351067", "−0.196988557"], ["EHI_052860", "C4M3S5", "XP_650458.1", "Heat‐shock protein 70_ putative", "−5.496325809", "−5.150576387", "0.345749422"], ["Not detected"], ["EHI_187770", "C4M9I2", "XP_656019.2", "SH3 domain protein", "‐", "‐", "0.303916225"], ["EHI_096420", "C4LWF5", "XP_656918.1", "LIM zinc finger domain‐containing protein (LimA)", "‐", "‐", "−0.78774608"], ["EHI_178470", "C4LUR3", "XP_653036.1", "CPBP6", "‐", "‐", "−0.611080228"], ["Gene expression and protein modification"], ["Increased"], ["EHI_050130", "C4LTQ0", "XP_657191.1", "60S ribosomal protein L14_ putative", "5.702363161", "1.648304292", "−4.054058869"], ["EHI_183480", "C4M727", "XP_654328.1", "60S ribosomal protein L27_ putative", "6.085963509", "5.75025083", "−0.335712679"], ["EHI_166800", "C4LY85", "XP_654066.2", "Ubiquitin_ putative", "3.628806975", "2.824287894", "−0.804519081"], ["Reduced"], ["EHI_006860", "C4LU56", "XP_650508.1", "60S ribosomal protein L5_ putative", "−5.900697969", "−6.602091887", "−0.701393918"], ["EHI_064710", "B1N384", "XP_001913650.1", "60S ribosomal protein L4_ putative", "−4.30857207", "−1.809131252", "2.499440818"], ["EHI_126920", "C4LWW8", "XP_651543.1", "Asparaginyl‐tRNA synthetase_ putative", "−5.641036285", "−2.595619356", "3.045416929"], ["EHI_073460", "C4LXL0", "XP_656678.1", "Glycyl‐tRNA synthetase_ putative", 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