Serglycin promotes proliferation, migration, and invasion via the JAK/STAT signaling pathway in osteosarcoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34493692", "kind": "science-record", "revision": 6, "content_hash": "sha256:0c594a6c1f75ad8fa8ae85adb5fca4f94609718d6e58338c513c4226522b3bb7", "created_at": "2026-11-01T15:41:51Z", "updated_at": "2026-11-01T21:26:10Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Serglycin promotes proliferation, migration, and invasion via the JAK/STAT signaling pathway in osteosarcoma.", "doi": "10.18632/aging.203392", "pmid": "34493692", "authors": ["Bin Lv", "Guangyu Gao", "Yuhong Guo", "Zhiping Zhang", "Renfeng Liu", "Zhengzai Dai", "Cheng Ju", "Yiping Liang", "Xiaofeng Tang", "Min Tang", "Xiao-Bin Lv"], "journal": "Aging", "year": 2021, "abstract": "BACKGROUND: Osteosarcoma (OS) is a common disease in the world, and its pathogenesis is still unclear. This study aims to identify the key genes that promote the proliferation, invasion, and metastasis of osteosarcoma cells.\nMETHOD: GSE124768 and GSE126209 were downloaded from the Gene Expression Omnibus (GEO) database. The gene ontology and enrichment pathway were analyzed by FunRich software. qPCR and Western blot were used to detect the gene expression. After gene knockdown, Transwell and wound healing assays were conducted on osteosarcoma cells to detect whether the genes were defined before enhancing the invasion of osteosarcoma.\nRESULTS: Totally, 341 mRNAs were found to be regulated differentially in osteosarcoma cells compared to osteoblasts. In addition, the expression level of Serglycin (SRGN) in osteosarcoma cells was higher than that in human osteoblasts. The invasion and proliferation ability of osteosarcoma cells with upregulated Serglycin was significantly increased, and on the contrary, decreased after Serglycin knockdown. Moreover, we preliminarily found that Serglycin may associate with the JAK/STAT signaling pathway.\nCONCLUSIONS: By using microarray and bioinformatics analyses, differently expressed mRNAs were identified and a complete gene network was constructed. 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Aft"], "order_basis": "paper_text_order:473", "id": "assay:2"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["ed to detect the gene expression. After gene knockdown, Transwell and wound healing assays were con", "d on the contrary, decreased after Serglycin knockdown. Moreover, we preliminarily found that Sergl"], "order_basis": "paper_text_order:537", "id": "assay:3"}, {"role": "data_generation", "definition_id": "microarray", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["TAT signaling pathway. CONCLUSIONS: By using microarray and bioinformatics analyses, differently exp"], "order_basis": "paper_text_order:1240", "id": "assay:4"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["dded 10% fetal bovine serum (FBS, Gibco) and cultured in a cell incubator with 5% CO2 and 85% relativ", "Professor Kang (Sun yat-sen university). We cultured osteosarcoma cells (MG63, U2OS, 143B, U2R, t", "dded 10% fetal bovine serum (FBS, Gibco) and cultured in a cell incubator with 5% CO2 and 85% rela", "sity). We cultured osteosarcoma cells (MG63, U2OS, 143B, U2R, they are common human osteosarco"], "order_basis": "paper_text_order:1563", "id": "assay:5"}]}