TTK (threonine tyrosine kinase) regulates the malignant behaviors of cancer cells and is regulated by microRNA-582-5p in ovarian cancer.

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The GEO2R method was employed to screen out the mRNAs that were abnormally expressed between OC tissues and normal ovarian tissues using three datasets from the Gene Expression Omnibus (GEO) database: GSE14407, GSE18520, and GSE36668. Moreover, the Kaplan-Meier plotter was utilized to investigate the association between TTK expression and OC patients' prognosis. Furthermore, quantitative real-time PCR (qRT-PCR) was applied to examine miR-582-5p expression and TTK mRNA expression in OC tissues and cells. Additionally, immunohistochemistry (IHC) experiment and Western blot were executed to examine TTK protein expression in OC tissues and cells, respectively. In addition, Cell Counting Kit-8 (CCK-8), transwell, and flow-cytometry experiments were performed to examine the multiplication, migration, and apoptosis of OC cells, respectively. In addition, dual-luciferase reporter gene tests were executed to validate the targeting relationship between miR-582-5p and TTK. We demonstrated that TTK expression was up-regulated in OC tissues and cells, and its overexpression was found to be associated with an adverse prognosis in OC patients. TTK overexpression enhanced OC cell multiplication and migration, and repressed apoptosis. Mechanistically, TTK was a downstream target of miR-582-5p. Furthermore, miR-582-5p overexpression impeded OC cell multiplication and migration, while TTK overexpression reversed this phenomenon. These data suggest that miR-582-5p and TTK are promising targets for OC diagnosis and therapy.", "claims": [{"id": "claim-method", "statement": "Kaplan–Meier plots and log-rank test were employed to perform survival analysis.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34516342", "locator": "sections[13] \"Statistical analysis\" paragraph 2", "quote": "Kaplan–Meier plots and log-rank test were employed to perform survival analysis."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34516342"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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Furthermore, quantitative real-time PCR (qRT-PCR) was applied to examine miR-582-5p"], "order_basis": "paper_text_order:684", "id": "assay:1"}, {"role": "data_generation", "definition_id": "ihc", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["ssion in OC tissues and cells. Additionally, immunohistochemistry (IHC) experiment and Western blot were execu", "d cells. Additionally, immunohistochemistry (IHC) experiment and Western blot were executed t", "an epithelial cell lines (HOSE) (Figure 1d). IHC staining revealed a higher positive rate of", "ell lines (HOSE) was detected by qRT-PCR. E. IHC staining was employed to detect TTK protein"], "order_basis": "paper_text_order:816", "id": "assay:2"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["y, immunohistochemistry (IHC) experiment and Western blot were executed to examine TTK protein express"], "order_basis": "paper_text_order:858", "id": "assay:3"}, {"role": "data_generation", "definition_id": "apoptosis_assay", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["o examine the multiplication, migration, and apoptosis of OC cells, respectively. In addition, dual", "multiplication and migration, and repressed apoptosis. Mechanistically, TTK was a downstream targe", "pectively. In addition, Cell Counting Kit-8 (CCK-8), transwell, and flow-cytometry experiments"], "order_basis": "paper_text_order:992", "id": "assay:4"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.77, "evidence": ["China). At 37°C with 5% CO2, all cells were cultured in Dulbecco’s modification of Eagle’s medium (D"], "order_basis": "paper_text_order:2203", "id": "assay:5"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["bad, CA, USA). SKOV-3 and OVCAR-3 cells were transfected with the oligonucleotides or plasmids with L", "d, NY, USA). pcDNA3.1-TTK, empty vector, TTK siRNA (si-TTK#1/si-TTK#2), scramble siRNA, miR-582", "tor, TTK siRNA (si-TTK#1/si-TTK#2), scramble siRNA, miR-582-5p mimics, miR-582-5p inhibitors an"], "order_basis": "paper_text_order:2483", "id": "assay:6"}, {"role": "analysis", "definition_id": "survival_analysis", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["logrank_test"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Kaplan–Meier plots and log-rank test were employed to perform survival analysis."], "order_basis": "consumes_earlier_output", "id": "assay:7"}]}