Biodata Mining of Differentially Expressed Genes between Acute Myocardial Infarction and Unstable Angina Based on Integrated Bioinformatics.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34568491", "kind": "science-record", "revision": 8, "content_hash": "sha256:0807bde4dbfc0b599e19c90327a46dcd515aedae5e2138eeff64b08cfcbcab57", "created_at": "2026-11-01T15:41:54Z", "updated_at": "2026-11-01T22:47:36Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Biodata Mining of Differentially Expressed Genes between Acute Myocardial Infarction and Unstable Angina Based on Integrated Bioinformatics.", "doi": "10.1155/2021/5584681", "pmid": "34568491", "authors": ["Siyu Guo", "Zhihong Huang", "Xinkui Liu", "Jingyuan Zhang", "Peizhi Ye", "Chao Wu", "Shan Lu", "Shanshan Jia", "Xiaomeng Zhang", "Xiuping Chen", "Miaomiao Wang", "Jiarui Wu"], "journal": "BioMed research international", "year": 2021, "abstract": "Acute coronary syndrome (ACS) is a complex syndrome of clinical symptoms. In order to accurately diagnose the type of disease in ACS patients, this study is aimed at exploring the differentially expressed genes (DEGs) and biological pathways between acute myocardial infarction (AMI) and unstable angina (UA). The GSE29111 and GSE60993 datasets containing microarray data from AMI and UA patients were downloaded from the Gene Expression Omnibus (GEO) database. DEG analysis of these 2 datasets is performed using the \"limma\" package in R software. DEGs were also analyzed using protein-protein interaction (PPI), Molecular Complex Detection (MCODE) algorithm, Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Correlation analysis and \"cytoHubba\" were used to analyze the hub genes. A total of 286 DEGs were obtained from GSE29111 and GSE60993, including 132 upregulated genes and 154 downregulated genes. Subsequent comprehensive analysis identified 20 key genes that may be related to the occurrence and development of AMI and UA and were involved in the inflammatory response, interaction of neuroactive ligand-receptor, calcium signaling pathway, inflammatory mediator regulation of TRP channels, viral protein interaction with cytokine and cytokine receptor, human cytomegalovirus infection, and cytokine-cytokine receptor interaction pathway. The integrated bioinformatical analysis could improve our understanding of DEGs between AMI and UA. The results of this study might provide a new perspective and reference for the early diagnosis and treatment of ACS.", "claims": [{"id": "claim-method", "statement": "DEG analysis of these 2 datasets is performed using the “limma” package in R software.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34568491", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "DEG analysis of these 2 datasets is performed using the “limma” package in R software."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation. All data obtained or analyzed during this study are available from the published article and its supplementary information files. 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The datasets during the current study are available from the corresponding author upon reasonable request."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34568491"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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Enrichment Analysis of DEGs", "row_count": 53, "column_count": 4}, "table": {"label": "Table 5", "caption": "Information on GO entries enriched by differentially expressed genes in GSE60993.", "rows": [["Category", "ID", "Item", "P adjust"], ["BP", "GO:0042119", "Neutrophil activation", "1.41E − 05"], ["BP", "GO:0045088", "Regulation of innate immune response", "6.17E − 05"], ["BP", "GO:0043312", "Neutrophil degranulation", "6.17E − 05"], ["BP", "GO:0002283", "Neutrophil activation involved in immune response", "6.17E − 05"], ["BP", "GO:0002446", "Neutrophil mediated immunity", "6.17E − 05"], ["BP", "GO:0035743", "CD4-positive, alpha-beta T cell cytokine production", "0.000114"], ["BP", "GO:0045089", "Positive regulation of innate immune response", "0.000148"], ["BP", "GO:0002367", "Cytokine production involved in immune response", "0.000235"], ["BP", "GO:0098542", "Defense response to other organisms", "0.000496"], ["BP", "GO:0002369", "T cell cytokine production", "0.000501"], ["BP", "GO:0002720", "Positive regulation of cytokine production involved in immune response", "0.001009"], ["BP", "GO:0030595", "Leukocyte chemotaxis", "0.001785"], ["BP", "GO:0002440", "Production of molecular mediator of immune response", "0.001785"], ["BP", "GO:0002699", "Positive regulation of immune effector process", "0.001886"], ["BP", "GO:0070673", "Response to interleukin-18", "0.001886"], ["BP", "GO:0002526", "Acute inflammatory response", "0.002469"], ["BP", "GO:0002690", "Positive regulation of leukocyte chemotaxis", "0.002469"], ["BP", "GO:0002718", "Regulation of cytokine production involved in immune response", "0.002508"], ["BP", "GO:0002456", "T cell mediated immunity", "0.002638"], ["BP", "GO:0002708", "Positive regulation of lymphocyte mediated immunity", "0.002638"], ["BP", "GO:0060337", "Type I interferon signaling pathway", "0.002638"], ["BP", "GO:0071357", "Cellular response to type I interferon", "0.002638"], ["BP", "GO:0042742", "Defense response to bacterium", 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