Integrated analysis of lncRNA-miRNA-mRNA ceRNA network in human aortic dissection.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34620091", "kind": "science-record", "revision": 5, "content_hash": "sha256:df7ecb12cc96109e7ad410c871c5ff6e55d3a876f51954582e40874d7aeb711e", "created_at": "2026-11-01T17:36:15Z", "updated_at": "2026-11-01T22:47:40Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Integrated analysis of lncRNA-miRNA-mRNA ceRNA network in human aortic dissection.", "doi": "10.1186/s12864-021-08012-3", "pmid": "34620091", "authors": ["Hao Zhang", "Ce Bian", "Simei Tu", "Fanxing Yin", "Panpan Guo", "Jian Zhang", "Xiaotong Song", "Qingyang Liu", "Chen Chen", "Yanshuo Han"], "journal": "BMC genomics", "year": 2021, "abstract": "BACKGROUND: Many studies on long chain non-coding RNAs (lncRNAs) are published in recent years. But the roles of lncRNAs in aortic dissection (AD) are still unclear and should be further examined. The present work focused on determining the molecular mechanisms underlying lncRNAs regulation in aortic dissection on the basis of the lncRNA-miRNA-mRNA competing endogenous RNA (ceRNA) network.\nMETHODS: This study collected the lncRNAs (GSE52093), mRNAs (GSE52093) and miRNAs (GSE92427) expression data within human tissue samples with aortic dissection group and normal group based on Gene Expression Omnibus (GEO) database.\nRESULTS: This study identified three differentially expressed lncRNAs (DELs), 19 differentially expressed miRNAs (DEmiRs) and 1046 differentially expressed mRNAs (DEGs) identified regarding aortic dissection. Furthermore, we constructed a lncRNA-miRNA-mRNA network through three lncRNAs (including two with up-regulation and one with down-regulation), five miRNAs (five with up-regulation), as well as 211 mRNAs (including 103 with up-regulation and 108 with down-regulation). Simultaneously, we conducted functional enrichment and pathway analyses on genes within the as-constructed ceRNA network. According to our PPI/ceRNA network and functional enrichment analysis results, four critical genes were found (E2F2, IGF1R, BDNF and PPP2R1B). In addition, E2F2 level was possibly modulated via lncRNA FAM87A-hsa-miR-31-5p/hsa-miR-7-5p or lncRNA C9orf106-hsa-miR-7-5p. The expression of IGF1R may be regulated by lncRNA FAM87A-hsa-miR-16-5p/hsa-miR-7-5p or lncRNA C9orf106-hsa-miR-7-5p.\nCONCLUSION: In conclusion, the ceRNA interaction axis we identified is a potentially critical target for treating AD. Our results shed more lights on the possible pathogenic mechanism in AD using a lncRNA-associated ceRNA network.", "claims": [{"id": "claim-method", "statement": "DEGs were analyzed by R package “Linear Models for Microarray Data (limma)” function for datasets and online analysis software SPSSAU.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34620091", "locator": "sections[4] \"DEGs analysis\" paragraph 2", "quote": "DEGs were analyzed by R package “Linear Models for Microarray Data (limma)” function for datasets and online analysis software SPSSAU."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets generated and analyzed during the current study are available in the Gene Expression Omnibus (GEO, https://www.ncbi.nlm.nih.gov/geo/) database (Accession Number: GSE52093, GSE92427).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34620091", "locator": "sections[21] \"Availability of data and materials\" paragraph 2", "quote": "The datasets generated and analyzed during the current study are available in the Gene Expression Omnibus (GEO, https://www.ncbi.nlm.nih.gov/geo/) database (Accession Number: GSE52093, GSE92427)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study identified three differentially expressed lncRNAs, 19 differentially expressed miRNAs, and 1046 differentially expressed mRNAs in aortic dissection using expression data obtained from public GEO datasets.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:34620091", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "This study identified three differentially expressed lncRNAs (DELs), 19 differentially expressed miRNAs (DEmiRs) and 1046 differentially expressed mRNAs (DEGs) identified regarding aortic dissection."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The study identified three differentially expressed lncRNAs, 19 differentially expressed miRNAs, and 1046 differentially expressed mRNAs in aortic dissection using expression data obtained from public GEO datasets.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34620091"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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