MMP14 Contributes to HDAC Inhibition-Induced Radiosensitization of Glioblastoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34638754", "kind": "science-record", "revision": 5, "content_hash": "sha256:e8b8c85ce6532ac5cfc800f84d56e513ed965491a900c9b5d920934ffb8bd909", "created_at": "2026-11-01T17:36:17Z", "updated_at": "2026-11-01T22:47:42Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "MMP14 Contributes to HDAC Inhibition-Induced Radiosensitization of Glioblastoma.", "doi": "10.3390/ijms221910403", "pmid": "34638754", "authors": ["Yuchuan Zhou", "Hongxia Liu", "Wang Zheng", "Qianping Chen", "Songling Hu", "Yan Pan", "Yang Bai", "Jianghong Zhang", "Chunlin Shao"], "journal": "International journal of molecular sciences", "year": 2021, "abstract": "Glioblastoma (GBM) is the most common and malignant primary brain tumor in adults. Radiotherapy has long been an important treatment method of GBM. However, the intrinsic radioresistance of GBM cells is a key reason of poor therapeutic efficiency. Recently, many studies have shown that using the histone deacetylase (HDAC) inhibitor suberoylanilide hydroxamic acid (SAHA) in radiotherapy may improve the prognosis of GBM patients, but the underlying molecular mechanisms remain unclear. In this study, Gene Expression Omnibus (GEO) datasets GSE153982 and GSE131956 were analyzed to evaluate radiation-induced changes of gene expression in GBM without or with SAHA treatment, respectively. Additionally, the survival-associated genes of GBM patients were screened using the Chinese Glioma Genome Atlas (CGGA) database. Taking the intersection of these three datasets, 11 survival-associated genes were discovered to be activated by irradiation and regulated by SAHA. The expressions of these genes were further verified in human GBM cell lines U251, T98G, and U251 homologous radioresistant cells (U251R) by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). It was found that MMP14 mRNA was considerably highly expressed in the radioresistant cell lines and was reduced by SAHA treatment. Transfection of MMP14 siRNA (siMMP14) suppressed cell survivals of these GBM cells after irradiation. Taken together, our results reveal for the first time that the MMP14 gene contributed to SAHA-induced radiosensitization of GBM.", "claims": [{"id": "claim-method", "statement": "DEG analysis of the two groups was performed using the Bioconductor package limma.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34638754", "locator": "sections[4] \"2.2. Screening of Survival-Associated Genes\" paragraph 2", "quote": "DEG analysis of the two groups was performed using the Bioconductor package limma."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data presented in this study are available on request from the corresponding author upon reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34638754", "locator": "sections[28] \"notes\" paragraph 1", "quote": "The data presented in this study are available on request from the corresponding author upon reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The MMP14 gene contributed to SAHA-induced radiosensitization of GBM.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:34638754", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Taken together, our results reveal for the first time that the MMP14 gene contributed to SAHA-induced radiosensitization of GBM."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The MMP14 gene contributed to SAHA-induced radiosensitization of GBM.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34638754"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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