GNG7 and ADCY1 as diagnostic and prognostic biomarkers for pancreatic adenocarcinoma through bioinformatic-based analyses.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34650124", "kind": "science-record", "revision": 5, "content_hash": "sha256:9e8d7e9f5bfbd00f1cc93736cbe9408492b7f7099c46d793cb6a3c85f39ac64b", "created_at": "2026-11-01T17:36:18Z", "updated_at": "2026-11-01T22:47:43Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "GNG7 and ADCY1 as diagnostic and prognostic biomarkers for pancreatic adenocarcinoma through bioinformatic-based analyses.", "doi": "10.1038/s41598-021-99544-x", "pmid": "34650124", "authors": ["Youfu Zhang", "Jinran Yang", "Xuyang Wang", "Xinchang Li"], "journal": "Scientific reports", "year": 2021, "abstract": "Pancreatic adenocarcinoma (PAAD) is one of the most lethal malignant tumors in the world. The GSE55643 and GSE15471 microarray datasets were downloaded to screen the diagnostic and prognostic biomarkers for PAAD. 143 downregulated genes and 118 upregulated genes were obtained. Next, we performed gene ontology (GO) and The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis on these genes and constructed a protein-protein interaction (PPI) network. We screened out two important clusters of genes, including 13 upregulated and 5 downregulated genes. After the survival analysis, 3 downregulated genes and 10 upregulated genes were identified as the selected key genes. The KEGG analysis on 13 selected genes showed that GNG7 and ADCY1 enriched in the Pathway in Cancer. Next, the diagnostic and prognostic value of GNG7 and ADCY1 was investigated using independent cohort of the Cancer Genome Atlas (TCGA), GSE84129 and GSE62452. We observed that the expression of the GNG7 and ADCY1 was decreased in PAAD. The diagnostic receiver operating characteristic (ROC) analysis indicated that the GNG7 and ADCY1 could serve as sensitive diagnostic markers in PAAD. Survival analysis suggested that expression of GNG7, ADCY1 were significantly associated with PAAD overall survival (OS). The multivariate cox regression analysis showed that the expression of GNG7, ADCY1 were independent risk factors for PAAD OS. Our study indicated GNG7 and ADCY1 may be potential diagnostic and prognostic biomarkers in patients with PAAD.", "claims": [{"id": "claim-method", "statement": "We utilized the R software and built-in limma packages (version 3.22.1; Fred Hutchinson Cancer Research Center) to perform DEGs analysis between the primary tumor tissue of PAAD patients and the paired adjacent non-malignant tissue.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34650124", "locator": "sections[4] \"Identification of DEGs\" paragraph 2", "quote": "We utilized the R software and built-in limma packages (version 3.22.1; Fred Hutchinson Cancer Research Center) to perform DEGs analysis between the primary tumor tissue of PAAD patients and the paired adjacent non-malignant tissue."}], "state": "complete"}, {"id": "claim-data-access", "statement": "All datasets generated for this study are available within the article.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34650124", "locator": "sections[22] \"Data availability\" paragraph 2", "quote": "All datasets generated for this study are available within the article."}], "state": "complete"}, {"id": "claim-target-result", "statement": "143 downregulated genes and 118 upregulated genes were obtained.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:34650124", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "143 downregulated genes and 118 upregulated genes were obtained."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "143 downregulated genes and 118 upregulated genes were obtained.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34650124"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE15471"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE15471"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:34650124", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1038/s41598-021-99544-x", "sha256": 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