Identification of MYH6 as the potential gene for human ischaemic cardiomyopathy.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34697898", "kind": "science-record", "revision": 5, "content_hash": "sha256:72a77b955e7bf628d7aeb74b0e930dedf2e620a5050574ffe95cd124d36c3227", "created_at": "2026-11-01T17:36:22Z", "updated_at": "2026-11-01T22:47:46Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identification of MYH6 as the potential gene for human ischaemic cardiomyopathy.", "doi": "10.1111/jcmm.17015", "pmid": "34697898", "authors": ["Jian-Hong Chen", "Lei-Li Wang", "Lin Tao", "Bin Qi", "Yong Wang", "Yu-Jie Guo", "Liu Miao"], "journal": "Journal of cellular and molecular medicine", "year": 2021, "abstract": "The present study aimed to explore the potential hub genes and pathways of ischaemic cardiomyopathy (ICM) and to investigate the possible associated mechanisms. Two microarray data sets (GSE5406 and GSE57338) were downloaded from the Gene Expression Omnibus (GEO) database. The limma package was used to analyse the differentially expressed genes (DEGs). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, Disease Ontology (DO) and Gene Ontology (GO) annotation analyses were performed. A protein-protein interaction (PPI) network was set up using Cytoscape software. Significant modules and hub genes were identified by the Molecular Complex Detection (MCODE) app. Then, further functional validation of hub genes in other microarrays and survival analysis were performed to judge the prognosis. A total of 1065 genes were matched, with an adjusted p < 0.05, and 17 were upregulated and 25 were downregulated with|log2 (fold change)|≥1.2. After removing the lengthy entries, GO identified 12 items, and 8 pathways were enriched at adjusted p < 0.05 (false discovery rate, FDR set at <0.05). Three modules with a score >8 after MCODE analysis and MYH6 were ultimately identified. When validated in GSE23561, MYH6 expression was lower in patients with CAD than in healthy controls (p < 0.05). GSE60993 data suggested that MYH6 expression was also lower in AMI patients (p < 0.05). In the GSE59867 data set, MYH6 expression was lower in CAD patients than in AMI patients and lower in heart failure (HF) patients than in non-HF patients. However, there was no difference at different periods within half a year, and HF was increased when MYH6 expression was low (p < 0.05-0.01). We performed an integrated analysis and validation and found that MYH6 expression was closely related to ICM and HF. However, whether this marker can be used as a predictor in blood samples needs further experimental verification.", "claims": [{"id": "claim-method", "statement": "We compared ICM subjects with healthy controls to identify the differentially expressed genes (DEGs) with the limma package in R.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34697898", "locator": "sections[4] \"Differentially expressed genes (DEGs) and functional enrichment analysis.\" paragraph 2", "quote": "We compared ICM subjects with healthy controls to identify the differentially expressed genes (DEGs) with the limma package in R."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data sets generated and/or analysed during the current study are publicly available.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34697898", "locator": "sections[20] \"DATA AVAILABILITY STATEMENT\" paragraph 2", "quote": "The data sets generated and/or analysed during the current study are publicly available."}], "state": "complete"}, {"id": "claim-target-result", "statement": "MYH6 expression was closely related to ischaemic cardiomyopathy and heart failure.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:34697898", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "We performed an integrated analysis and validation and found that MYH6 expression was closely related to ICM and HF."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "MYH6 expression was closely related to ischaemic cardiomyopathy and heart failure.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34697898"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE23561"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE23561"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:34697898", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1111/jcmm.17015", "sha256": 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