CDCA7 Facilitates Tumor Progression by Directly Regulating CCNA2 Expression in Esophageal Squamous Cell Carcinoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34737951", "kind": "science-record", "revision": 3, "content_hash": "sha256:fb3ddc485da6e82d06d8f71a178479920ac5790d8dec1b0da96daa4d609365a1", "created_at": "2026-11-01T17:36:25Z", "updated_at": "2026-11-01T21:26:33Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "CDCA7 Facilitates Tumor Progression by Directly Regulating CCNA2 Expression in Esophageal Squamous Cell Carcinoma.", "doi": "10.3389/fonc.2021.734655", "pmid": "34737951", "authors": ["Hongyi Li", "Yongjia Weng", "Shaojie Wang", "Fang Wang", "Yanqiang Wang", "Pengzhou Kong", "Ling Zhang", "Caixia Cheng", "Heyang Cui", "Enwei Xu", "Shuqing Wei", "Dinghe Guo", "Fei Chen", "Yanghui Bi", "Yongsheng Meng", "Xiaolong Cheng", "Yongping Cui"], "journal": "Frontiers in oncology", "year": 2021, "abstract": "BACKGROUND: CDCA7 is a copy number amplified gene identified not only in esophageal squamous cell carcinoma (ESCC) but also in various cancer types. Its clinical relevance and underlying mechanisms in ESCC have remained unknown.\nMETHODS: Tissue microarray data was used to analyze its expression in 179 ESCC samples. The effects of CDCA7 on proliferation, colony formation, and cell cycle were tested in ESCC cells. Real-time PCR and Western blot were used to detect the expression of its target genes. Correlation of CDCA7 with its target genes in ESCC and various SCC types was analyzed using GSE53625 and TCGA data. The mechanism of CDCA7 was studied by chromatin immunoprecipitation (ChIP), luciferase reporter assays, and rescue assay.\nRESULTS: The overexpression of CDCA7 promoted proliferation, colony formation, and cell cycle in ESCC cells. CDCA7 affected the expression of cyclins in different cell phases. GSE53625 and TCGA data showed CCNA2 expression was positively correlated with CDCA7. The knockdown of CCNA2 reversed the malignant phenotype induced by CDCA7 overexpression. Furthermore, CDCA7 was found to directly bind to CCNA2, thus promoting its expression.\nCONCLUSIONS: Our results reveal a novel mechanism of CDCA7 that it may act as an oncogene by directly upregulating CCNA2 to facilitate tumor progression in ESCC.", "claims": [{"id": "claim-method", "statement": "GraphPad Prism software was used to analyze the correlations between CDCA7 and CCNA2 using non-parametric correlation (Spearman).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34737951", "locator": "sections[17] \"Statistical Analyses\" paragraph 2", "quote": "GraphPad Prism software was used to analyze the correlations between CDCA7 and CCNA2 using non-parametric correlation (Spearman)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Publicly available datasets were analyzed in this study. These data can be found here: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE53625, https://xenabrowser.net/, and https://www.cbioportal.org/.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34737951", "locator": "sections[26] \"Data Availability Statement\" paragraph 2", "quote": "Publicly available datasets were analyzed in this study. These data can be found here: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE53625, https://xenabrowser.net/, and https://www.cbioportal.org/."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Our results reveal a novel mechanism of CDCA7 that it may act as an oncogene by directly upregulating CCNA2 to facilitate tumor progression in ESCC.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:34737951", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "Our results reveal a novel mechanism of CDCA7 that it may act as an oncogene by directly upregulating CCNA2 to facilitate tumor progression in ESCC."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Our results reveal a novel mechanism of CDCA7 that it may act as an oncogene by directly upregulating CCNA2 to facilitate tumor progression in ESCC.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34737951"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE53625"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE53625"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:34737951", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.3389/fonc.2021.734655", "sha256": "sha256:e81fbea6676ada360a1bd25433eea1dfe0cfdb032023c9c4a0252564a749e4b7", "bytes": 55651, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.3389/fonc.2021.734655", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC8561731", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE53625", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE53625", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE53625", "version": null, "note": "Publicly available datasets were analyzed in this study. 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METHODS: Tissue microarray data was used to analyze its expression in 1"], "order_basis": "paper_text_order:316", "id": "assay:1"}, {"role": "data_generation", "definition_id": "qpcr", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["n, and cell cycle were tested in ESCC cells. Real-time PCR and Western blot were used to detect the exp"], "order_basis": "paper_text_order:486", "id": "assay:2"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["were tested in ESCC cells. Real-time PCR and Western blot were used to detect the expression of its ta"], "order_basis": "paper_text_order:504", "id": "assay:3"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.85, "evidence": ["on was positively correlated with CDCA7. The knockdown of CCNA2 reversed the malignant phenotype in"], "order_basis": "paper_text_order:1076", "id": "assay:4"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["entific, Inc., Waltham, MA, USA). All of the cell lines were cultured at 37°C, 5% CO2. The culture medium was repl", "nes KYSE150, KYSE180, KYSE450, and TE-1 were cultured in HyClone™ RPMI-1640 medium, and the cell line", "ium, and the cell lines HET-1A and 293T were cultured in HyClone™ DMEM/High Glucose medium (GE Health", "c., Waltham, MA, USA). The cell line NE3 was cultured in a 1:1 mixture of EpiLife medium (Cascade Bio"], "order_basis": "paper_text_order:2576", "id": "assay:5"}]}