Identification of Key Pathways and Genes in SARS-CoV-2 Infecting Human Intestines by Bioinformatics Analysis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34787756", "kind": "science-record", "revision": 4, "content_hash": "sha256:f6c9876cde08b5cbf8d2eb0aafed61443a1be3d0c6502e1ebaa39062ae2cbaeb", "created_at": "2026-11-01T17:36:29Z", "updated_at": "2026-11-01T22:05:10Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identification of Key Pathways and Genes in SARS-CoV-2 Infecting Human Intestines by Bioinformatics Analysis.", "doi": "10.1007/s10528-021-10144-w", "pmid": "34787756", "authors": ["Ji-Chun Chen", "Tian-Ao Xie", "Zhen-Zong Lin", "Yi-Qing Li", "Yu-Fei Xie", "Zhong-Wei Li", "Xu-Guang Guo"], "journal": "Biochemical genetics", "year": 2022, "abstract": "COVID-19 is a serious infectious disease that has recently swept the world, and research on its causative virus, SARS-CoV-2, remains insufficient. Therefore, this study uses bioinformatics analysis techniques to explore the human digestive tract diseases that may be caused by SARS-CoV-2 infection. The gene expression profile data set, numbered GSE149312, is from the Gene Expression Omnibus (GEO) database and is divided into a 24-h group and a 60-h group. R software is used to analyze and screen out differentially expressed genes (DEGs) and then gene ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses are performed. In KEGG, the pathway of non-alcoholic fatty liver disease exists in both the 24-h group and 60-h group. STRING is used to establish a protein-protein interaction (PPI) network, and Cytoscape is then used to visualize the PPI and define the top 12 genes of the node as the hub genes. Through verification, nine statistically significant hub genes are identified: AKT1, TIMP1, NOTCH, CCNA2, RRM2, TTK, BUB1B, KIF20A, and PLK1. In conclusion, the results of this study can provide a certain direction and basis for follow-up studies of SARS-CoV-2 infection of the human digestive tract and provide new insights for the prevention and treatment of diseases caused by SARS-CoV-2.", "claims": [{"id": "claim-method", "statement": "Then GSE150728 was analyzed by bioinformatics, and the data obtained passed t test and non-parametric test and P < 0.05 was used as the standard to screen for statistically significant hub genes.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34787756", "locator": "sections[7] \"Verification of the Hub Genes\" paragraph 2", "quote": "Then GSE150728 was analyzed by bioinformatics, and the data obtained passed t test and non-parametric test and P < 0.05 was used as the standard to screen for statistically significant hub genes."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Below is the link to the electronic supplementary material.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34787756", "locator": "sections[16] \"Supplementary Information\" paragraph 1", "quote": "Below is the link to the electronic supplementary material."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Through verification, nine statistically significant hub genes were identified: AKT1, TIMP1, NOTCH, CCNA2, RRM2, TTK, BUB1B, KIF20A, and PLK1.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:34787756", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Through verification, nine statistically significant hub genes are identified: AKT1, TIMP1, NOTCH, CCNA2, RRM2, TTK, BUB1B, KIF20A, and PLK1."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Through verification, nine statistically significant hub genes were identified: AKT1, TIMP1, NOTCH, CCNA2, RRM2, TTK, BUB1B, KIF20A, and PLK1.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34787756"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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