Screening and Analysis of Key Genes in miRNA-mRNA Regulatory Network of Membranous Nephropathy.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34824764", "kind": "science-record", "revision": 4, "content_hash": "sha256:035ae751c44fdd99c51b26c1802247c9cca555320bb4d8bc9d747f98ac9b60a7", "created_at": "2026-11-01T17:36:31Z", "updated_at": "2026-11-01T22:05:14Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Screening and Analysis of Key Genes in miRNA-mRNA Regulatory Network of Membranous Nephropathy.", "doi": "10.1155/2021/5331948", "pmid": "34824764", "authors": ["Yawei Hou", "Yameng Li", "Yichuan Wang", "Wenpu Li", "Zhenwei Xiao"], "journal": "Journal of healthcare engineering", "year": 2021, "abstract": "Background: MicroRNAs (miRNAs) are confirmed to participate in occurrence, development, and prevention of membranous nephropathy (MN), but their mechanism of action is unclear.\nObjective: With the GEO database and the use of bioinformatics, miRNA-mRNA regulatory network genes relevant to MN were explored and their potential mechanism of action was explained.\nMethods: The MN-related miRNA chip data set (GSE51674) and mRNA chip data set (GSE108109) were downloaded from the GEO database. Differential analysis was performed using the GEO2R online tool. TargetScan, miRTarBase, and StarBase databases were used to predict potential downstream target genes regulated by differentially expressed miRNAs, and the intersection with differential genes were taken to obtain candidate target genes. According to the regulatory relationship between miRNA and mRNA, the miRNA-mRNA relationship pair was clarified and Cytoscape was used to construct a miRNA-mRNA regulatory network. WebGestalt was used to conduct enrichment analysis of the biological process of differential mRNAs in the regulatory network; FunRich analyzes the differential mRNA pathways in the miRNA-mRNA regulatory network. And the STRING database was used to construct a PPI network for candidate target genes, and Cytoscape visually analyzes the PPI network.\nResults: Experiments were conducted to screen differentially expressed miRNAs and mRNAs. There were 30 differentially expressed miRNAs, including 22 upregulated and 8 downregulated; and 1267 differentially expressed mRNAs, including 536 upregulated and 731 downregulated. Using TargetScan, miRTarBase, and StarBase databases to predict the downstream targets of differentially expressed miRNAs, 2957 downstream target genes coexisting in the 3 databases were predicted to intersect with differentially expressed mRNAs to obtain 175 candidate target genes. Finally, 36 miRNA-mRNA relationship pairs comprising 10 differentially expressed miRNAs and 27 differentially expressed mRNAs were screened out, and the regulatory network was constructed. Further analysis revealed that the miRNA regulatory network genes may be involved in the development of membranous nephropathy by mTOR, PDGFR-β, LKB1, and VEGF/VEGFR signaling pathways.\nConclusion: The miRNA regulatory network genes may participate in the regulation of podocyte autophagy, lipid metabolism, and renal fibrosis through mTOR, PDGFR-β, LKB1, and VEGF/VEGFR signaling pathways, thereby affecting the occurrence and development of membranous nephropathy.", "claims": [{"id": "claim-method", "statement": "The online analysis tool GEO2R (https://www.ncbi.nlm.nih.gov/geo/geo2r/) from NCBI's GEO online analysis tool was used to obtain differential mRNAs and miRNAs and screen the differential genes. The standard setting is adj. p value <0.01 and |log2 fold change (FC)| > 1.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34824764", "locator": "sections[7] \"2.4.1. Data Processing and Differential Expression Analysis\" paragraph 2", "quote": "The online analysis tool GEO2R (https://www.ncbi.nlm.nih.gov/geo/geo2r/) from NCBI's GEO online analysis tool was used to obtain differential mRNAs and miRNAs and screen the differential genes. The standard setting is adj. p value <0.01 and |log2 fold change (FC)| > 1."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The simulation experiment data used to support the findings of this study are available from the corresponding author upon request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34824764", "locator": "sections[21] \"Data Availability\" paragraph 2", "quote": "The simulation experiment data used to support the findings of this study are available from the corresponding author upon request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Finally, 36 miRNA-mRNA relationship pairs comprising 10 differentially expressed miRNAs and 27 differentially expressed mRNAs were screened out, and the regulatory network was constructed.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:34824764", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "Finally, 36 miRNA-mRNA relationship pairs comprising 10 differentially expressed miRNAs and 27 differentially expressed mRNAs were screened out, and the regulatory network was constructed."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Finally, 36 miRNA-mRNA relationship pairs comprising 10 differentially expressed miRNAs and 27 differentially expressed mRNAs were screened out, and the regulatory network was constructed.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34824764"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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