Quantitative Analysis of the Cardiac Phosphoproteome in Response to Acute β-Adrenergic Receptor Stimulation In Vivo.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34830474", "kind": "science-record", "revision": 3, "content_hash": "sha256:a82eac1ce50a20e0bc58cdc4d9502dc6e898f9b4faccd6056c1250c1487d232f", "created_at": "2026-11-01T17:36:32Z", "updated_at": "2026-11-01T21:26:41Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Quantitative Analysis of the Cardiac Phosphoproteome in Response to Acute β-Adrenergic Receptor Stimulation In Vivo.", "doi": "10.3390/ijms222212584", "pmid": "34830474", "authors": ["Alican Güran", "Yanlong Ji", "Pan Fang", "Kuan-Ting Pan", "Henning Urlaub", "Metin Avkiran", "Christof Lenz"], "journal": "International journal of molecular sciences", "year": 2021, "abstract": "β-adrenergic receptor (β-AR) stimulation represents a major mechanism of modulating cardiac output. In spite of its fundamental importance, its molecular basis on the level of cell signalling has not been characterised in detail yet. We employed mass spectrometry-based proteome and phosphoproteome analysis using SuperSILAC (spike-in stable isotope labelling by amino acids in cell culture) standardization to generate a comprehensive map of acute phosphoproteome changes in mice upon administration of isoprenaline (ISO), a synthetic β-AR agonist that targets both β1-AR and β2-AR subtypes. Our data describe 8597 quantitated phosphopeptides corresponding to 10,164 known and novel phospho-events from 2975 proteins. In total, 197 of these phospho-events showed significantly altered phosphorylation, indicating an intricate signalling network activated in response to β-AR stimulation. In addition, we unexpectedly detected significant cardiac expression and ISO-induced fragmentation of junctophilin-1, a junctophilin isoform hitherto only thought to be expressed in skeletal muscle. Data are available via ProteomeXchange with identifier PXD025569.", "claims": [{"id": "claim-method", "statement": "Two-sample t-test in the volcano plot was performed to filter the significantly regulated proteins and phospho-events with the Permutation-based FDR less than 0.5% and S0 (background variability) of 0.1.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34830474", "locator": "sections[19] \"4.6. 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Regulation of Cardiac Protein Phosphorylation in Response to Acute β-AR Stimulation\" paragraph 5", "quote": "We identified 197 phospho-events from 143 different proteins that displayed a statistically significant increase or decrease in phosphorylation in mice that received ISO, relative to saline controls (Figure 3)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Acute isoprenaline stimulation significantly increased or decreased phosphorylation at 197 phospho-events from 143 proteins in mouse ventricular tissue.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34830474"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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Preparation of Mouse Tissue Samples Mice were anaesthetized with 1.5% isoflurane. Core bod", "led mouse hearts from two 10-week-old female mice were purchased from Silantes (Munich, Germany). T"], "order_basis": "paper_text_order:63", "id": "assay:1"}, {"role": "data_generation", "definition_id": "mass_spectrometry", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["een characterised in detail yet. 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