Circular RNA circ_0000517 Facilitates The Growth and Metastasis of Non-Small Cell Lung Cancer by Sponging miR-326/miR-330-5p.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34837683", "kind": "science-record", "revision": 5, "content_hash": "sha256:47d1f9b4d46320632806f8e799c8b659806028c8d868a073c440f01578b271e7", "created_at": "2026-11-01T17:36:32Z", "updated_at": "2026-11-01T22:47:57Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Circular RNA circ_0000517 Facilitates The Growth and Metastasis of Non-Small Cell Lung Cancer by Sponging miR-326/miR-330-5p.", "doi": "10.22074/cellj.2021.7913", "pmid": "34837683", "authors": ["Qiyan Tan", "Changyu Liu", "Ying Shen", "Tao Huang"], "journal": "Cell journal", "year": 2021, "abstract": "OBJECTIVE: There is growing evidence showing that circular RNAs (circRNAs) are crucial regulators in modulating the biological behavior of tumors. This work is aimed to probe the role of circ_0000517 in non-small cell lung cancer (NSCLC) and to elucidate its mechanism of action.\nMATERIALS AND METHODS: In this experimental study, the differentially expressed circRNAs in NSCLC were screened using the GEO database (GSE158695). Circ_0000517, miR-326, miR-330-5p, and MMP2 expression levels were determined by quantitative real-time polymerase chain reaction (qRT-PCR) analysis and Western blot. The proliferation, apoptosis, migration, and invasion of NSCLC cells were detected by CCK-8, flow cytometry, and transwell assays. RNA immunoprecipitation (RIP), RNA pull-down, and dual-luciferase reporter gene assays were performed to clarify the association between the circ_0000517 and miR-326/miR-330-5p.\nRESULTS: Circ_0000517 was shown to be up-regulated in NSCLC tissues and cell lines. The up-regulation of circ_0000517 is closely associated with advanced clinical stage of cancer, lymph node metastasis, and poor prognosis in NSCLC patients. Circ_0000517 knockdown impeded the proliferation, migration, and invasion of NSCLC cells and enhanced their apoptosis. Mechanistically, circ_0000517 was demonstrated to up-regulate MMP2 expression via decoying miR-326 and miR-330-5p to facilitate the malignant biological behaviors of NSCLC cells.\nCONCLUSION: This work reveals that circ_0000517 is implicated in NSCLC cell growth and metastasis through the modulation of miR-326/miR-330-5p/MMP2, providing novel insights into the role of circRNAs in NSCLC progression.", "claims": [{"id": "claim-method", "statement": "Student’s t test and one-way ANOVA were used to analyze the difference between two groups and among multiple groups, respectively. Correlations were measured by Pearson’s correlation analysis. Chi-square test was perform to analyze the association between clinical characteristics and circ_0000517 expression levels. Kaplan-Meier survival curve was used to compare the prognosis of the NSCLC patients.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34837683", "locator": "sections[16] \"Statistical analysis\" paragraph 2", "quote": "Student’s t test and one-way ANOVA were used to analyze the difference between two groups and among multiple groups, respectively. Correlations were measured by Pearson’s correlation analysis. Chi-square test was perform to analyze the association between clinical characteristics and circ_0000517 expression levels. Kaplan-Meier survival curve was used to compare the prognosis of the NSCLC patients."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Gene expression data of GSE158695 were obtained from the NCBI GEO database and analyzed using the online software GEO2R to screen for differentially expressed circRNAs. GSE158695 contained 6 human samples, including 3 cases of NSCLC tissues and 3 cases of paracancerous tissues.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34837683", "locator": "sections[5] \"Bioinformatics analysis\" paragraph 2", "quote": "Gene expression data of GSE158695 were obtained from the NCBI GEO database and analyzed using the online software GEO2R to screen for differentially expressed circRNAs. GSE158695 contained 6 human samples, including 3 cases of NSCLC tissues and 3 cases of paracancerous tissues."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Circ_0000517 up-regulates MMP2 expression by decoying miR-326 and miR-330-5p, thereby facilitating malignant biological behaviors in NSCLC cells.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:34837683", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "Mechanistically, circ_0000517 was demonstrated to up-regulate MMP2 expression via decoying miR-326 and miR-330-5p to facilitate the malignant biological behaviors of NSCLC cells."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Circ_0000517 up-regulates MMP2 expression by decoying miR-326 and miR-330-5p, thereby facilitating malignant biological behaviors in NSCLC cells.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34837683"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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