Construction of miRNA-mRNA network reveals crucial miRNAs and genes in acute myocardial infarction.

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The microarray datasets GSE31568, GSE148153, and GSE66360 were downloaded from the Gene Expression Omnibus (GEO) database. We identified differentially expressed miRNAs (DE-miRNAs) and mRNAs (DE-mRNAs) in AMI samples compared with normal control samples. The consistently changing miRNAs in both GSE31568 and GSE148153 datasets were selected as candidate DE-miRNAs. The interactions between the candidate DE-miRNAs and DE-mRNAs were analyzed, and a miRNA-mRNA network and a protein-protein interaction network were constructed, along with functional enrichment and pathway analyses. A total of 209 DE-miRNAs in the GSE31568 dataset, 857 DE-miRNAs in the GSE148153 dataset, and 351 DE-mRNAs in the GSE66360 dataset were identified. Eighteen candidate DE-miRNAs were selected from both the GSE31568 and GSE148153 datasets. Furthermore, miR-646, miR-127-5p, miR-509-5p, miR-509-3-5p, and miR-767-5p were shown to have a higher degree in the miRNA-mRNA network. THBS-1 as well as FOS was a hub gene in the miRNA-mRNA network and the protein-protein interaction (PPI) network, respectively. CDKN1A was important in both miRNA-mRNA network and PPI network. We established a miRNA-mRNA network in AMI and identified five miRNAs and three genes, which might be used as biomarkers and potential therapeutic targets for patients with AMI.", "claims": [{"id": "claim-method", "statement": "The limma package of R-studio was used to obtain the DE-miRNAs and DE-mRNAs between AMI and control samples.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34857679", "locator": "sections[4] \"Differential expression analysis\" paragraph 2", "quote": "The limma package of R-studio was used to obtain the DE-miRNAs and DE-mRNAs between AMI and control samples."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Reanalysis of the public datasets identified differentially expressed miRNAs and mRNAs in acute myocardial infarction and supported an AMI miRNA-mRNA regulatory network.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:34857679", "locator": "sections[11] \"Identification of DE-miRNAs and DE-mRNAs\" paragraph 2", "quote": "After the data preprocessing, 209 DE-miRNAs (94 upregulated and 115 downregulated) in the GSE31568 dataset (Fig. 2A) and 857 DE-miRNAs (191 upregulated and 666 downregulated) in the GSE148153 dataset (Fig. 2B), as well as 351 DE-mRNAs (289 upregulated and 62 downregulated) in the GSE66360 dataset (Fig. 2C and D) were identified, consequently."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "Reanalysis of the public datasets identified differentially expressed miRNAs and mRNAs in acute myocardial infarction and supported an AMI miRNA-mRNA regulatory network.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34857679"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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