Circular RNA circRPPH1 promotes breast cancer progression via circRPPH1-miR-512-5p-STAT1 axis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34873163", "kind": "science-record", "revision": 4, "content_hash": "sha256:d7a2757ac39366467b74cf6eec5243398389c465dcc7cf4ffa55d0c312d9e1f9", "created_at": "2026-11-01T17:36:35Z", "updated_at": "2026-11-01T22:05:18Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Circular RNA circRPPH1 promotes breast cancer progression via circRPPH1-miR-512-5p-STAT1 axis.", "doi": "10.1038/s41420-021-00771-y", "pmid": "34873163", "authors": ["Yixiang Huang", "Wenfang Zheng", "Changle Ji", "Xuehui Wang", "Yunhe Yu", "Xiaochong Deng", "Xiqian Zhou", "Lin Fang"], "journal": "Cell death discovery", "year": 2021, "abstract": "Breast cancer (BC) is one of the most fatal diseases among women all over the world. Non-coding RNAs including circular RNAs (circRNAs) have been reported to be involved in different aspects during tumorigenesis and progression. In this study, we aimed to explore the biological functions and underlying mechanism of circRPPH1 in BC. Candidate circRNAs were screened in dataset GSE101123 from Gene Expression Omnibus (GEO) database and a differentially expressed circRNA, circRPPH1, was discovered in BC. CircRPPH1 expression was higher in the cancerous tissue compared to paired adjacent tissue. Further in vitro and in vivo experiments indicated that circRPPH1 acted as an oncogene in BC. In addition, circRPPH1 was mainly localized in cytoplasm and played the role of miR-512-5p sponge. By sequestering miR-512-5p from the 3'-UTR of STAT1, circRPPH1 inhibited the suppressive role of miR-512-5p, stabilized STAT1 mRNA in BC and finally affected BC progression. In conclusion, these findings indicated that circRPPH1 acted as an oncogene and regulated BC progression via circRPPH1-miR-512-5p-STAT1 axis, which might provide a potential therapeutic target for BC treatment.", "claims": [{"id": "claim-method", "statement": "Differential expression analysis was conducted by R studio and the results were showed in volcano plot and box plot.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34873163", "locator": "sections[12] \"Bioinformatic analysis\" paragraph 2", "quote": "Differential expression analysis was conducted by R studio and the results were showed in volcano plot and box plot."}], "state": "complete"}, {"id": "claim-data-access", "statement": "All data and materials are available under request by contacting with the corresponding author.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34873163", "locator": "sections[35] \"notes\" paragraph 1", "quote": "All data and materials are available under request by contacting with the corresponding author."}], "state": "complete"}, {"id": "claim-target-result", "statement": "In conclusion, these findings indicated that circRPPH1 acted as an oncogene and regulated BC progression via circRPPH1-miR-512-5p-STAT1 axis, which might provide a potential therapeutic target for BC treatment.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:34873163", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "In conclusion, these findings indicated that circRPPH1 acted as an oncogene and regulated BC progression via circRPPH1-miR-512-5p-STAT1 axis, which might provide a potential therapeutic target for BC treatment."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "In conclusion, these findings indicated that circRPPH1 acted as an oncogene and regulated BC progression via circRPPH1-miR-512-5p-STAT1 axis, which might provide a potential therapeutic target for BC treatment.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34873163"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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Further in vitro and in vivo experiments indicated that circRPPH1 acted a"], "order_basis": "paper_text_order:668", "id": "assay:1"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["ll patients were available. Cell culture and transfection Normal breast epithelial cell line (MCF-10A)", "000 (Invitrogen, USA) was used for transient transfection following the manufacturer’s instructions. S", "designed by HarO Life (Shanghai, China) and transfected with agents from ZorinBio (Shanghai, China)."], "order_basis": "paper_text_order:1752", "id": "assay:2"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["l Basal Medium (MEBM) (Cambrex, USA). The BC cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM,", "f Sciences (Shanghai, China). All cells were cultured in a 5% CO2 incubator at 37 °C. The medium for", "MEBM) (Cambrex, USA). The BC cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gi", "(MCF-10A) and BC cell lines (MDA-MB-231 and MCF-7) were acquired from the Chinese Academy of S"], "order_basis": "paper_text_order:1845", "id": "assay:3"}, {"role": "data_generation", "definition_id": "apoptosis_assay", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": [". Two-way ANOVA was used for the analysis of MTT assay. 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