Influence of N6-Methyladenosine Modification Gene HNRNPC on Cell Phenotype in Parkinson's Disease.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34966539", "kind": "science-record", "revision": 5, "content_hash": "sha256:1ed7be301b0c69116802696f0b8802cece81cc5ae25157c18a2934cd40b319f8", "created_at": "2026-11-01T17:36:40Z", "updated_at": "2026-11-01T22:48:08Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Influence of N6-Methyladenosine Modification Gene HNRNPC on Cell Phenotype in Parkinson's Disease.", "doi": "10.1155/2021/9919129", "pmid": "34966539", "authors": ["Wei Quan", "Jia Li", "Li Liu", "Qinghui Zhang", "Yidan Qin", "Xiaochen Pei", "Jiajun Chen"], "journal": "Parkinson's disease", "year": 2021, "abstract": "This study aimed to explore the N6-methyladenosine (m6A) modification genes involved in the pathogenesis of Parkinson's disease (PD) through data analysis of the two data sets GSE120306 and GSE22491 in the GEO database and further explore its influence on cell phenotype in PD. We analyzed the differentially expressed genes and function enrichment analysis of the two sets of data and found that the expression of the m6A-modification gene HNRNPC was significantly downregulated in the PD group, and it played an important role in DNA metabolism, RNA metabolism, and RNA processing and may be involved in PD. Then, we constructed the HNRNPC differential expression cell line to study the role of this gene in the pathogenesis of PD. The results showed that overexpression of HNRNPC can promote the proliferation of PC12 cells, inhibit their apoptosis, and inhibit the expression of inflammatory factors IFN-β, IL-6, and TNF-α, suggesting that HNRNPC may cause PD by inhibiting the proliferation of dopaminergic nerve cells, promoting their apoptosis, and causing immune inflammation. Our study also has certain limitations. For example, the data of the experimental group and the validation group come from different cell types, and the data of the experimental group involve individuals with G2019S LRRK2 mutations. In addition, due to the low expression of HNRNPC in PC12 cells, we used the method of overexpressing this gene to study its function. All these factors may cause our conclusions to be biased. Therefore, more research is still needed to corroborate it in the future.", "claims": [{"id": "claim-method", "statement": "We used the classic Bayesian method provided by the limma package (Version 3.30.13, http://www.bioconductor.org/packages/2.9/bioc/html/limma.html) to analyze differentially expressed genes (DEGs) on the two sets of data.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34966539", "locator": "sections[3] \"2.1. Data Processing and DEG Screening\" paragraph 3", "quote": "We used the classic Bayesian method provided by the limma package (Version 3.30.13, http://www.bioconductor.org/packages/2.9/bioc/html/limma.html) to analyze differentially expressed genes (DEGs) on the two sets of data."}], "state": "complete"}, {"id": "claim-data-access", "statement": "In this study, the gene expression datasets analyzed were obtained from the GEO database (https://www.ncbi.nlm.nih.gov/geo/). After a careful review, we choose the transcriptome data GSE120306 as the experimental group and another set of chip data GSE22491 as a verification group. The experimental platform is GPL6480 Agilent-014850 Whole Human Genome Microarray 4 × 44K G4112F (Probe Name version). The data are freely available online.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34966539", "locator": "sections[20] \"Data Availability\" paragraph 2", "quote": "In this study, the gene expression datasets analyzed were obtained from the GEO database (https://www.ncbi.nlm.nih.gov/geo/). After a careful review, we choose the transcriptome data GSE120306 as the experimental group and another set of chip data GSE22491 as a verification group. The experimental platform is GPL6480 Agilent-014850 Whole Human Genome Microarray 4 × 44K G4112F (Probe Name version). The data are freely available online."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The results showed that overexpression of HNRNPC can promote the proliferation of PC12 cells, inhibit their apoptosis, and inhibit the expression of inflammatory factors IFN-β, IL-6, and TNF-α.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:34966539", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The results showed that overexpression of HNRNPC can promote the proliferation of PC12 cells, inhibit their apoptosis, and inhibit the expression of inflammatory factors IFN-β, IL-6, and TNF-α, suggesting that HNRNPC may cause PD by inhibiting the proliferation of dopaminergic nerve cells, promoting their apoptosis, and causing immune inflammation."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "The results showed that overexpression of HNRNPC can promote the proliferation of PC12 cells, inhibit their apoptosis, and inhibit the expression of inflammatory factors IFN-β, IL-6, and TNF-α.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34966539"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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The cells were cultured in RPMI-1640 medium containing 1% penicillin-st", "dium was changed once every 2-3 days, 1 : 2- passaged in a ratio of 1 : 4. Gas phase was as follow"], "order_basis": "paper_text_order:870", "id": "assay:1"}, {"role": "data_generation", "definition_id": "apoptosis_assay", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["e proliferation of PC12 cells, inhibit their apoptosis, and inhibit the expression of inflammatory", "of dopaminergic nerve cells, promoting their apoptosis, and causing immune inflammation. Our study"], "order_basis": "paper_text_order:896", "id": "assay:2"}, {"role": "data_generation", "definition_id": "rna_seq", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["hy individuals. This data set corresponds to RNA-Seq profiles of human iPSC-derived midbrain-patt", "ase from the GEO public database: one set is transcriptome data GSE120306, including four PD patients a"], "order_basis": "paper_text_order:1805", "id": "assay:3"}, {"role": "data_generation", "definition_id": "microarray", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["is GPL6480 Agilent-014850 Whole Human Genome Microarray 4 × 44K G4112F (Probe Name version). This gr"], "order_basis": "paper_text_order:2407", "id": "assay:4"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["d to m6A modification. 2.4. Cell Culture and Transfection Rat adrenal medullary pheochromocytoma cells", "to about 80%, we used lipofectamine 2000 for transfection and changed the medium 6 hours after transfe", "fection and changed the medium 6 hours after transfection. Cells were harvested for 48 hours after tra", "ion. Cells were harvested for 48 hours after transfection. 2.7. 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