Identification of a five-miRNA signature as a novel potential prognostic biomarker in patients with nasopharyngeal carcinoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:34998434", "kind": "science-record", "revision": 5, "content_hash": "sha256:8233b69a5677b0e9f3a17cbff23fddf64e31e78692bd6d013ee4d228e136c0d7", "created_at": "2026-11-01T17:36:42Z", "updated_at": "2026-11-01T22:48:11Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identification of a five-miRNA signature as a novel potential prognostic biomarker in patients with nasopharyngeal carcinoma.", "doi": "10.1186/s41065-021-00214-9", "pmid": "34998434", "authors": ["Bo Tu", "Ling Ye", "Qingsong Cao", "Sisi Gong", "Miaohua Jiang", "Hui Li"], "journal": "Hereditas", "year": 2022, "abstract": "BACKGROUND: MicroRNAs (miRNAs) are involved in the prognosis of nasopharyngeal carcinoma (NPC). This study used clinical data and expression data of miRNAs to develop a prognostic survival signature for NPC patients to detect high-risk subject.\nRESULTS: We identified 160 differentially expressed miRNAs using RNA-Seq data from the GEO database. Cox regression model consisting of hsa-miR-26a, hsa-let-7e, hsa-miR-647, hsa-miR-30e, and hsa-miR-93 was constructed by the least absolute contraction and selection operator (LASSO) in the training set. All the patients were classified into high-risk or low-risk groups by the optimal cutoff value of the 5-miRNA signature risk score, and the two risk groups demonstrated significant different survival. The 5-miRNA signature showed high predictive and prognostic accuracies. The results were further confirmed in validation and external validation set. Results from multivariate Cox regression analysis validated 5-miRNA signature as an independent prognostic factor. A total of 13 target genes were predicted to be the target genes of miRNA target genes. Both PPI analysis and KEGG analysis networks were closely related to tumor signaling pathways. The prognostic model of mRNAs constructed using data from the dataset GSE102349 had higher AUCs of the target genes and higher immune infiltration scores of the low-risk groups. The mRNA prognostic model also performed well on the independent immunotherapy dataset Imvigor210.\nCONCLUSIONS: This study constructed a novel 5-miRNA signature for prognostic prediction of the survival of NPC patients and may be useful for individualized treatment of NPC patients.", "claims": [{"id": "claim-method", "statement": "We plotted survival curves using Kaplan-Meier (KM), and log-rank tests were conducted to evaluate survival differences in the two groups. Receiver operating characteristic curve (ROC) was established using “timeROC” software package [33], and we calculated area under the curve (AUC) value to analyze model sensitivity and specificity.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34998434", "locator": "sections[20] \"Evaluation of the riskcore in GEO dataset\" paragraph 2", "quote": "We plotted survival curves using Kaplan-Meier (KM), and log-rank tests were conducted to evaluate survival differences in the two groups. Receiver operating characteristic curve (ROC) was established using “timeROC” software package [33], and we calculated area under the curve (AUC) value to analyze model sensitivity and specificity."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets generated and/or analyzed during the current study are available in the [GSE32960] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE32960], in [GSE70970] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi], in [GSE36682] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi], in [GSE118721] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE118721], and in [GSE102349] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:34998434", "locator": "sections[29] \"notes\" paragraph 1", "quote": "The datasets generated and/or analyzed during the current study are available in the [GSE32960] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE32960], in [GSE70970] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi], in [GSE36682] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi], in [GSE118721] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE118721], and in [GSE102349] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi]."}], "state": "complete"}, {"id": "claim-target-result", "statement": "This study constructed a novel 5-miRNA signature for prognostic prediction of the survival of NPC patients and may be useful for individualized treatment of NPC patients.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:34998434", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "This study constructed a novel 5-miRNA signature for prognostic prediction of the survival of NPC patients and may be useful for individualized treatment of NPC patients."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "This study constructed a novel 5-miRNA signature for prognostic prediction of the survival of NPC patients and may be useful for individualized treatment of NPC patients.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:34998434"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE102349"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE102349"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:34998434", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1186/s41065-021-00214-9", "sha256": 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[https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE32960], in [GSE70970] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi], in [GSE36682] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi], in [GSE118721] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE118721], and in [GSE102349] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi].", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The datasets generated and/or analyzed during the current study are available in the [GSE32960] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE32960], in [GSE70970] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi], in [GSE36682] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi], in [GSE118721] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE118721], and in [GSE102349] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi].", 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in [GSE118721] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE118721], and in [GSE102349] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi].", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The datasets generated and/or analyzed during the current study are available in the [GSE32960] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE32960], in [GSE70970] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi], in [GSE36682] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi], in [GSE118721] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE118721], and in [GSE102349] repository [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi].", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM816038", "GSM816039", "GSM816040", "GSM816041", "GSM816042", "GSM816043", "GSM816044", "GSM816045", "GSM816046", "GSM816047", "GSM816048", "GSM816049", 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