Prognostic Significance of CCNB2 Expression in Triple-Negative Breast Cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35002325", "kind": "science-record", "revision": 4, "content_hash": "sha256:ddb737064e83dfa74e16996ef21c9e584d4db1c61b581a71f28d72f38ee9e957", "created_at": "2026-11-01T17:36:43Z", "updated_at": "2026-11-01T22:05:32Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Prognostic Significance of CCNB2 Expression in Triple-Negative Breast Cancer.", "doi": "10.2147/CMAR.S339105", "pmid": "35002325", "authors": ["Jintao Cao", "Shuai Sun", "Rui Min", "Ran Li", "Xingyu Fan", "Yuexin Han", "Zhenzhong Feng", "Nan Li"], "journal": "Cancer management and research", "year": 2021, "abstract": "PURPOSE: The aim of this study was to explore potential gene therapy targets for triple-negative breast cancer (TNBC).\nPATIENTS AND METHODS: Three gene expression profiles (GSE64790, GSE62931, and GSE38959) from the Gene Expression Omnibus (GEO) database were analyzed. The GEO2R analysis tool was used to screen for differentially expressed genes (DEGs) between TNBC and normal tissues, followed by Gene Ontology functional annotation and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of the DEGs. The protein-protein interaction network of DEGs was visualized using Metascape to identify the core genes. Subsequently, transcriptional data for the core genes in patients with breast cancer were investigated in the ONCOMINE database. Kaplan-Meier survival analysis was used to evaluate the prognostic value of core gene expression levels in patients with TNBC. Finally, the clinicopathological and long-term follow-up data of 39 patients with TNBC were retrospectively analyzed at the First Affiliated Hospital of the Bengbu Medical College between January 2014 and July 2020. Immunohistochemistry was used to evaluate the expression and subcellular localization of CCNB2 in TNBC tissues.\nRESULTS: A total of 66 DEGs were identified between TNBC and normal tissues, including 33 upregulated and 33 downregulated genes in TNBC. Furthermore, a potential protein complex was identified for five core genes. The high expression of these core genes, especially the overexpression of CCNB2, was correlated with a poor prognosis of patients with TNBC. The CCNB2 protein was expressed in the cytoplasm, and its expression was significantly higher in TNBC tissues than that in the adjacent nontumor tissues. Overall survival of patients was significantly correlated with the expression of CCNB2 (p < 0.05).\nCONCLUSION: CCNB2 may play a crucial role in the development of TNBC and has the potential to be used as a prognostic biomarker for TNBC.", "claims": [{"id": "claim-method", "statement": "The Kaplan–Meier method was used for univariate overall survival (OS) analysis.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35002325", "locator": "sections[10] \"Statistical Analysis\" paragraph 2", "quote": "The Kaplan–Meier method was used for univariate overall survival (OS) analysis."}], "state": "complete"}, {"id": "claim-data-access", "statement": "No additional data are available.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35002325", "locator": "sections[23] \"Data Sharing Statement\" paragraph 2", "quote": "No additional data are available."}], "state": "complete"}, {"id": "claim-target-result", "statement": "High expression of the five core genes, especially CCNB2, was associated with poor prognosis in patients with TNBC.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35002325", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "Furthermore, a potential protein complex was identified for five core genes. The high expression of these core genes, especially the overexpression of CCNB2, was correlated with a poor prognosis of patients with TNBC."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "High expression of the five core genes, especially CCNB2, was associated with poor prognosis in patients with TNBC.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35002325"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE38959"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE38959"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:35002325", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.2147/CMAR.S339105", "sha256": 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