Proteomics Investigations of Potential Protein Biomarkers in Sera of Rabbits Infected With Schistosoma japonicum.

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The primary pathological damage from the disease is granuloma and fibrosis caused by egg aggregation, and early treatment can effectively prevent the occurrence of liver fibrosis. Therefore, it is very important to identify biomarkers that can be used for early diagnosis of Schistosoma japonicum infection. In this study, a label-free proteomics method was performed to observe the alteration of proteins before infection, 1 and 6 weeks after infection, and 5 and 7 weeks after treatment. A total of 10 proteins derived from S. japonicum and 242 host-derived proteins were identified and quantified as significantly changed. Temporal analysis was carried out to further analyze potential biomarkers with coherent changes during infection and treatment. The results revealed biological process changes in serum proteins compared to infection and treatment groups, which implicated receptor-mediated endocytosis, inflammatory response, and acute-phase response such as mannan-binding lectin serine peptidase 1, immunoglobulin, and collagen. These findings offer guidance for the in-depth analysis of potential biomarkers of schistosomiasis, host protein, and early diagnosis of S. japonicum and its pathogenesis. Data are available via ProteomeXchange with identifier PXD029635.", "claims": [{"id": "claim-method", "statement": "The proteins that were significantly up- or downregulated (two-sided, unpaired Student’s t-test with equal variance assumed, p-adjust< 0.05, n = 3, | log2FC | > | log21.5 |) in at least one infected sample compared with the corresponding Ctrl are shown.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35004354", "locator": "sections[7] \"Statistical Analysis of Mass Spectrometry\" paragraph 3", "quote": "The proteins that were significantly up- or downregulated (two-sided, unpaired Student’s t-test with equal variance assumed, p-adjust< 0.05, n = 3, | log2FC | > | log21.5 |) in at least one infected sample compared with the corresponding Ctrl are shown."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry proteomics data are available in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD029635.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35004354", "locator": "sections[15] \"Data Availability Statement\" paragraph 2", "quote": "The mass spectrometry proteomics data are available in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD029635."}], "state": "complete"}, {"id": "claim-target-result", "statement": "A total of 10 proteins derived from S. japonicum and 242 host-derived proteins were identified and quantified as significantly changed.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35004354", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "A total of 10 proteins derived from S. japonicum and 242 host-derived proteins were identified and quantified as significantly changed."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "A total of 10 proteins derived from S. japonicum and 242 host-derived proteins were identified and quantified as significantly changed.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35004354"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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In this study, a label-free proteomics method was performed to observe the alterati", "l., 2015) by Blast2GO. The mass spectrometry proteomics data have been deposited to the ProteomeXcha", "mple was calculated. Statistical Analysis of Mass Spectrometry All the original MS data were processed usin"], "order_basis": "paper_text_order:0", "id": "assay:1"}, {"role": "analysis", "definition_id": "group_comparison", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["The proteins that were significantly up- or downregulated (two-sided, unpaired Student’s t-test with equal variance assumed, p-adjust< 0.05, n = 3, | log2FC | > | log21.5 |) in at least one infected sample compared with the corresponding Ctrl are shown."], "order_basis": "consumes_earlier_output", "id": "assay:2"}]}