Comparative secretome analysis of Staphylococcus aureus strains with different within-herd intramammary infection prevalence.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35030987", "kind": "science-record", "revision": 3, "content_hash": "sha256:1f6c248e414bb565b8c9f6f19d10a8fcce52bd61f12315b16125439a28466f04", "created_at": "2026-11-01T17:36:45Z", "updated_at": "2026-11-01T21:26:59Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Comparative secretome analysis of Staphylococcus aureus strains with different within-herd intramammary infection prevalence.", "doi": "10.1080/21505594.2021.2024014", "pmid": "35030987", "authors": ["M Filippa Addis", "Salvatore Pisanu", "Valentina Monistero", "Alessandra Gazzola", "Martina Penati", "Joel Filipe", "Susanna Di Mauro", "Paola Cremonesi", "Bianca Castiglioni", "Paolo Moroni", "Daniela Pagnozzi", "Sebastiana Tola", "Renata Piccinini"], "journal": "Virulence", "year": 2022, "abstract": "Staphylococcus aureus is a major pathogen causing intramammary infection and mastitis in dairy cows. S. aureus genotypes (GT) can differ significantly in their ability to diffuse and persist in the herd; while the association of virulence gene carriage with epidemiological behavior remains unclear, a role for secreted proteins has been postulated. We characterized the secretome of six S. aureus strains belonging to two genotypes with opposite within-herd prevalence, GTB (high) and GTS (low), corresponding to sequence types (ST) 8 and 398, by high-resolution tandem mass spectrometry and differential analysis with Proteome Discoverer. Data are available via ProteomeXchange with identifier PXD029571. Out of 720 identified proteins, 98 were unique or more abundant in GTB/ST8 and 68 in GTS/ST398. GTB/ST8 released more immunoglobulin-binding proteins, complement and antimicrobial peptide inhibitors, enterotoxins, and metabolic enzymes, while GTS/ST398 released more leukocidins, hemolysins, lipases, and peptidases. Furthermore, GTB/ST8 released the von Willebrand factor protein, staphylokinase, and clumping factor B, while GTS released the staphylococcal coagulase and clumping factor A. Hence, GTB/ST8 secretomes indicated a higher propensity for immune evasion and chronicity and GTS/ST398 secretomes for cellular damage and inflammation, consistent with their epidemiological characteristics. Accordingly, GTS/ST398 secretions were significantly more cytotoxic against bovine PBMCs in vitro. Our findings confirm the crucial role of extracellular virulence factors in S. aureus pathogenesis and highlight the need to investigate their differential release adding to gene carriage for a better understanding of the relationship of S. aureus genotypes with epidemiological behavior and, possibly, disease severity.", "claims": [{"id": "claim-data-access", "statement": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium [65] via the PRIDE partner repository [66] with the dataset identifier PXD029571.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35030987", "locator": "sections[20] \"Data availability\" paragraph 2", "quote": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium [65] via the PRIDE partner repository [66] with the dataset identifier PXD029571."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Differential secretome analysis identified 166 proteins with significantly different abundance between the two S. aureus genotypes, with 98 more abundant in GTB/ST8 and 68 in GTS/ST398.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35030987", "locator": "sections[14] \"Differential shotgun proteomics; general results\" paragraph 2", "quote": "Upon differential proteomic analysis with Proteome Discoverer, 166 proteins showed a significantly different abundance between the two genotypes: 98 were more abundant in GTB/ST8 and 68 in GTS/ST398 (Figure 3(b))."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Differential secretome analysis identified 166 proteins with significantly different abundance between the two S. aureus genotypes, with 98 more abundant in GTB/ST8 and 68 in GTS/ST398.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35030987"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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