Presence of periodontal pathogenic bacteria in blood of patients with coronary artery disease.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35075206", "kind": "science-record", "revision": 6, "content_hash": "sha256:6b2dd56ea1b78aca749a72de9655e1b0b7c1c571cd5c9d1bbea12078a78a4cbc", "created_at": "2026-11-01T17:36:48Z", "updated_at": "2026-11-01T22:48:18Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Presence of periodontal pathogenic bacteria in blood of patients with coronary artery disease.", "doi": "10.1038/s41598-022-05337-1", "pmid": "35075206", "authors": ["Zuray Corredor", "Andrés Suarez-Molina", "Cristian Fong", "Laura Cifuentes-C", "Sandra Guauque-Olarte"], "journal": "Scientific reports", "year": 2022, "abstract": "It has been hypothesised that oral bacteria can migrate, through the blood, from the mouth to the arterial plaques, thus exacerbating atherosclerosis. This study compared bacteria present in the peripheral blood of individuals with and without coronary artery disease (CAD). RNA sequences obtained from blood were downloaded from GEO (GSE58150). Eight patients with coronary artery calcification (CAC) scoring > 500 and eight healthy individuals were analysed. After conducting quality control, the sequences were aligned to the hg38 reference genome using Hisat2. Bacterial taxa were analysed by inputting the unmapped sequences into Kraken. Ecological indices were calculated using Vegan. The package DESeq2 was used to compare the counts of bacteria per standard rank between groups. A total of 51 species were found only in patients with CAD and 41 were exclusively present in healthy individuals. The counts of one phylum, one class, three orders, two families and one genus were significantly different between the analysed groups (p < 0.00032, FDR < 10%), including the orders Cardiobacteriales, Corynebacteriales and Fusobacteriales. Twenty-three bacterial species belonging to the subgingival plaque bacterial complexes were also identified in the blood of individuals from both the groups; Fusobacterium nucleatum was significantly less frequent in patients with CAD (p = 0.0012, FDR = 4.8%). Furthermore, the frequency of another 11 bacteria differed significantly among patients with CAD than that among healthy individuals (p < 0.0030, FDR < 10%). These bacteria have not been previously reported in patients with atherosclerosis and periodontitis. The presence of members of the subgingival plaque bacterial complexes in the blood of patients with CAC supports the hypothesis that the periodontopathogens can be disseminated through the blood flow to other body parts where they may enhance inflammatory processes that can lead to the development or exacerbation of atherosclerosis.", "claims": [{"id": "claim-method", "statement": "DESeq2 v.1.28.1 package was used to compare the counts of bacteria per standard rank (phyla, class, order, family, genus and species) between the two groups.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35075206", "locator": "sections[5] \"Comparison of taxa between CAD and healthy groups\" paragraph 2", "quote": "DESeq2 v.1.28.1 package was used to compare the counts of bacteria per standard rank (phyla, class, order, family, genus and species) between the two groups."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets analysed during the current study are available in the NCBI GEO database (accession number: GSE58150, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE58150).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35075206", "locator": "sections[19] \"Data availability\" paragraph 2", "quote": "The datasets analysed during the current study are available in the NCBI GEO database (accession number: GSE58150, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE58150)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The counts of one phylum, one class, three orders, two families and one genus were significantly different between the analysed groups (p < 0.00032, FDR < 10%), including the orders Cardiobacteriales, Corynebacteriales and Fusobacteriales.", "kind": "quantitative", "target": {"value": 10.0, "unit": "per cent", "tolerance": null, "source": "The counts of one phylum, one class, three orders, two families and one genus were significantly different between the analysed groups (p < 0.00032, FDR < 10%), including the orders Cardiobacteriales, Corynebacteriales and Fusobacteriales."}, "evidence": [{"asset": "source:pmid:35075206", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The counts of one phylum, one class, three orders, two families and one genus were significantly different between the analysed groups (p < 0.00032, FDR < 10%), including the orders Cardiobacteriales, Corynebacteriales and Fusobacteriales."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The counts of one phylum, one class, three orders, two families and one genus were significantly different between the analysed groups (p < 0.00032, FDR < 10%), including the orders Cardiobacteriales, Corynebacteriales and Fusobacteriales.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35075206"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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