Hsa-miR-557 Inhibits Osteosarcoma Growth Through Targeting KRAS.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35087570", "kind": "science-record", "revision": 5, "content_hash": "sha256:3905bef355e6d693fbfb2034e217c032fcc2f97a97ab2f13f49a97c538cdb7e6", "created_at": "2026-11-01T17:36:48Z", "updated_at": "2026-11-01T22:05:41Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Hsa-miR-557 Inhibits Osteosarcoma Growth Through Targeting KRAS.", "doi": "10.3389/fgene.2021.789823", "pmid": "35087570", "authors": ["Zhi Qiao", "Jinfeng Li", "Hongwei Kou", "Xiangrong Chen", "Deming Bao", "Guowei Shang", "Songfeng Chen", "Yanhui Ji", "Tian Cheng", "Yisheng Wang", "Hongjian Liu"], "journal": "Frontiers in genetics", "year": 2021, "abstract": "Objective: Osteosarcoma is the most common malignancy in the skeletal system; studies showed an important role of miRNAs in tumorigenesis, indicating miRNAs as possible therapeutic molecules. This study found abnormal hsa-miR-557 expression levels in osteosarcoma and tried to explore the potential function and the mechanism. Methods: Differential expression genes of osteosarcoma were analyzed using GSE28423 from the GEO database. Survival analysis of miRNAs was conducted with data obtained from the TARGET-OS database. STRING and miRDIP were used to predict target genes of hsa-miR-557; KRAS was then verified using dual-luciferase reporter assay. Expression of genes was detected by qPCR, and levels of proteins were detected by Western blot. The proliferation ability of cells was detected by CCK-8 and cell cycle analysis. Tumor formation assay in nude mice was used to detect the influence of osteosarcoma by hsa-miR-557 in vivo. Results: Analysis from the GEO and TARGET databases found 12 miRNAs that are significantly related to the osteosarcoma prognosis, 7 downregulated (hsa-miR-140-3p, hsa-miR-564, hsa-miR-765, hsa-miR-1224-5p, hsa-miR-95, hsa-miR-940, and hsa-miR-557) and 5 upregulated (hsa-miR-362-3p, hsa-miR-149, hsa-miR-96, hsa-miR-744, and hsa-miR-769-5p). CCK-8 analysis and cell cycle analysis found that hsa-miR-557 could significantly inhibit the proliferation of osteosarcoma cells. The tumor formation assay in nude mice showed that tumor sizes and weights were inhibited by hsa-miR-557 transfection. Further studies also proved that hsa-miR-557 could target the 3'UTR of KRAS and modulate phosphorylation of downstream proteins. Conclusion: This study showed that hsa-miR-557 could inhibit osteosarcoma growth both in vivo and in vitro, by modulating KRAS expression.", "claims": [{"id": "claim-method", "statement": "Survival analysis of miRNAs was conducted with data obtained from the TARGET-OS database.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35087570", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "Survival analysis of miRNAs was conducted with data obtained from the TARGET-OS database."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/Supplementary Material.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35087570", "locator": "sections[22] \"Data Availability Statement\" paragraph 2", "quote": "The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/Supplementary Material."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The GEO and TARGET analyses identified 12 miRNAs significantly related to osteosarcoma prognosis, including 7 downregulated miRNAs such as hsa-miR-557.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35087570", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "Analysis from the GEO and TARGET databases found 12 miRNAs that are significantly related to the osteosarcoma prognosis, 7 downregulated (hsa-miR-140-3p, hsa-miR-564, hsa-miR-765, hsa-miR-1224-5p, hsa-miR-95, hsa-miR-940, and hsa-miR-557) and 5 upregulated (hsa-miR-362-3p, hsa-miR-149, hsa-miR-96, hsa-miR-744, and hsa-miR-769-5p)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The GEO and TARGET analyses identified 12 miRNAs significantly related to osteosarcoma prognosis, including 7 downregulated miRNAs such as hsa-miR-557.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35087570"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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