The promotional effect of microRNA-103a-3p in cervical cancer cells by regulating the ubiquitin ligase FBXW7 function.

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Herein, the differentially expressed miRNAs that were screened based on GSE55940 microarray data retrieved from Gene Expression Omnibus (GEO), and miR-103a-3p was significantly upregulated in CC tissues which was selected as the target miRNA for further research. We also found that high expression of miR-103a-3p was closely associated with histological grades, FIGO stage and distant metastasis as well as reflected poor overall survival. Moreover, miR-103a-3p inhibition decreased the growth capacity of SiHa and HeLa cells by inducing cell apoptosis. And F-box and WD repeat-domain containing protein 7 (FBXW7), a well-known tumor suppressor in many cancer types, was identified as a direct target of miR-103a-3p. It was further found that FBXW7 was significantly downregulated in CC tissues, and it was inversely correlated with miR-103a-3p expression levels. Further investigation demonstrated that FBXW7 upregulation could simulate the roles of miR-103a-3p knockdown in cell viability and apoptosis. Moreover, FBXW7 knockdown efficiently abrogated the influences of CC cells proliferation caused by miR-103a-3p inhibition. Notably, miR-103a-3p could block FBXW7 mediated the downstream transcription factor pathways. Taken together, these findings suggest that miR-103a-3p functions as an oncogene in CC by targeting FBXW7.", "claims": [{"id": "claim-method", "statement": "Differences between two groups were analyzed by unpaired t test and continuous data from multiple groups were analyzed using a one-way ANOVA, followed by Tukey’s post hoc test. The correlation between FBXW7 and miR-103a-3p expression was analyzed using Pearson’s correlation coefficient. Survival curves were calculated by the Kaplan–Meier method by the log-rank test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35094292", "locator": "sections[14] \"Statistical analysis\" paragraph 2", "quote": "Differences between two groups were analyzed by unpaired t test and continuous data from multiple groups were analyzed using a one-way ANOVA, followed by Tukey’s post hoc test. The correlation between FBXW7 and miR-103a-3p expression was analyzed using Pearson’s correlation coefficient. Survival curves were calculated by the Kaplan–Meier method by the log-rank test."}], "state": "complete"}, {"id": "claim-data-access", "statement": "All data generated and/or analyzed during the present study are included in this article.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35094292", "locator": "sections[25] \"Data availability\" paragraph 2", "quote": "All data generated and/or analyzed during the present study are included in this article."}], "state": "complete"}, {"id": "claim-target-result", "statement": "miR-103a-3p functions as an oncogene in cervical cancer by targeting FBXW7.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35094292", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Taken together, these findings suggest that miR-103a-3p functions as an oncogene in CC by targeting FBXW7."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "miR-103a-3p functions as an oncogene in cervical cancer by targeting FBXW7.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35094292"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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And F-box", ". SiHa, HeLa, C-33A and Ect1/E6E7 cells were cultured in Dulbecco’s Modified Eagle (DMEM, Hyclone, Lo", "St. Louis, MO, USA), while CasKi cells were cultured in RPMI 1640 (Gibco, Grand Island, NY, USA) con", "NY, USA) containing 10% FBS. All cells were cultured in humidified 37 °C incubator with 5% CO2. Stat"], "order_basis": "paper_text_order:814", "id": "assay:1"}, {"role": "data_generation", "definition_id": "apoptosis_assay", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["city of SiHa and HeLa cells by inducing cell apoptosis. And F-box and WD repeat-domain containing p", "miR-103a-3p knockdown in cell viability and apoptosis. 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