EMG1 interacts with NOP14 to regulate the growth, migration, and invasion of melanoma cells via the Wnt/β-catenin pathway.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35117729", "kind": "science-record", "revision": 5, "content_hash": "sha256:67d7f7b743cdbba6334ff7371757b60a6cce2b9bb3a21869fec397359c083053", "created_at": "2026-11-01T17:36:52Z", "updated_at": "2026-11-01T22:48:24Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "EMG1 interacts with NOP14 to regulate the growth, migration, and invasion of melanoma cells via the Wnt/β-catenin pathway.", "doi": "10.21037/tcr.2020.03.79", "pmid": "35117729", "authors": ["Jingrong Li", "Rui Zhao", "Yunlong Guo", "Ruihua Fang"], "journal": "Translational cancer research", "year": 2020, "abstract": "BACKGROUND: The role of EMG1 in the progression of malignant melanoma remains unclear.\nMETHODS: Expression levels of EMG1 in melanoma tissues from GEO and TCGA databases was analyzed. The role of EMG1 was determined by overexpression on cell growth, apoptosis, migration and invasion. Glutathione S-transferase (GST) pulldown and co-immunoprecipitation (CoIP) assays were applied to reveal the relationship of EMG1 and nucleolar complex protein 14 (NOP14).\nRESULTS: The expression level of EMG1 was downregulated in melanoma tissues in GSE7553 dataset and further decreased in tissues from metastasis patients from both GEE7553 and TCGA cohorts. Overexpression of EMG1 suppressed proliferation, promoted apoptosis, and inhibited migration and invasion in melanoma cells. EMG1 interacted with NOP14 and functioned together to regulate the growth, apoptosis, migration and invasion of cultured melanoma cells. Furthermore, simultaneous overexpression of EMG1 and NOP14 decreased the levels of WNT3a, β-catenin, phosphorylated-GSK-3β, and c-Myc.\nCONCLUSIONS: EMG1 and NOP14 inhibit melanoma cell proliferation, migration, and invasion by regulating the Wnt/β-catenin signaling pathway.", "claims": [{"id": "claim-method", "statement": "Student’s t-tests were used for comparisons of two groups, and one-way analysis of variance (ANOVA) was used for multiple group comparisons. The correlations between EMG1 protein levels and clinicopathological characteristics of melanoma patients were analyzed by chi-squared tests.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35117729", "locator": "sections[9] \"Statistical analysis\" paragraph 2", "quote": "Student’s t-tests were used for comparisons of two groups, and one-way analysis of variance (ANOVA) was used for multiple group comparisons. The correlations between EMG1 protein levels and clinicopathological characteristics of melanoma patients were analyzed by chi-squared tests."}], "state": "complete"}, {"id": "claim-data-access", "statement": "EMG1 expression in the Riker melanoma dataset was analyzed using the public database Oncomine (40).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35117729", "locator": "sections[11] \"EMG1 expression is associated with melanoma\" paragraph 2", "quote": "EMG1 expression in the Riker melanoma dataset was analyzed using the public database Oncomine (40)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "EMG1 and NOP14 inhibit melanoma cell proliferation, migration, and invasion by regulating the Wnt/β-catenin signaling pathway.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35117729", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "EMG1 and NOP14 inhibit melanoma cell proliferation, migration, and invasion by regulating the Wnt/β-catenin signaling pathway."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "EMG1 and NOP14 inhibit melanoma cell proliferation, migration, and invasion by regulating the Wnt/β-catenin signaling pathway.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35117729"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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Glutathione S-trans", "n of EMG1 suppressed proliferation, promoted apoptosis, and inhibited migration and invasion in mel", "functioned together to regulate the growth, apoptosis, migration and invasion of cultured melanoma"], "order_basis": "paper_text_order:328", "id": "assay:1"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["ells/well) were plated in 24-well plates and transfected with NOP14 or EMG1 overexpression plasmids u", "EMG1 overexpression plasmids using FuGENE HD transfection reagent (Roche Applied Science, USA). The ce", "ls were harvested for analysis after 48 h of transfection. 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