Proteome data of neuroblastoma cells overexpressing Neuroglobin.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35128003", "kind": "science-record", "revision": 5, "content_hash": "sha256:8bb80e6023ef93281d97706de89d94fe9077afb092be8b6f3b10badef675d15a", "created_at": "2026-11-01T17:36:53Z", "updated_at": "2026-11-01T22:05:49Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Proteome data of neuroblastoma cells overexpressing Neuroglobin.", "doi": "10.1016/j.dib.2022.107843", "pmid": "35128003", "authors": ["Michele Costanzo", "Marianna Caterino", "Illari Salvatori", "Valeria Manganelli", "Alberto Ferri", "Roberta Misasi", "Margherita Ruoppolo"], "journal": "Data in brief", "year": 2022, "abstract": "In this article, we present data on the proteome of human neuroblastoma cells stably overexpressing Neuroglobin (NGB). The neuroprotective role of NGB is clearly established, nevertheless the related mechanistic processes, which are dependent on NGB overexpression, are not known. To address this question, we performed shotgun label-free quantification (LFQ) proteomics using an SH-SY5Y cell model of neuroblastoma that overexpresses an NGB-FLAG construct, and wild type cells transfected with an empty vector as control (CTRL). The proteomes from six biological samples per condition were digested using the S-Trap sample preparation followed by LC-MS/MS analysis with a LTQ-Orbitrap XL mass spectrometer. The quantitative analysis was performed using the LFQ algorithm of MaxQuant, leading to 1654 correctly quantified proteins over 2580 identified proteins. Finally, the statistic comparison of the two analyzed groups within Perseus platform identified 178 differential proteins (107 up- and 71 down-regulated). In addition, multivariate statistical analysis was carried out using MetaboAnalyst 5.0 software. MS proteomics data are available via ProteomeXchange with the dataset identifier PXD029012.", "claims": [{"id": "claim-method", "statement": "Finally, the comparison between the NGB-FLAG and CTRL conditions was carried out selecting significant proteins with values of S0 = 0.5 and Student's t-test with FDR = 0.01.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35128003", "locator": "sections[6] \"Proteomic data analysis\" paragraph 2", "quote": "Finally, the comparison between the NGB-FLAG and CTRL conditions was carried out selecting significant proteins with values of S0 = 0.5 and Student's t-test with FDR = 0.01."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The raw mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD029012 (available at https://www.ebi.ac.uk/pride/archive/projects/PXD029012)", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35128003", "locator": "sections[1] \"Specifications Table\" paragraph 2", "quote": "The raw mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD029012 (available at https://www.ebi.ac.uk/pride/archive/projects/PXD029012)"}], "state": "complete"}, {"id": "claim-target-result", "statement": "The statistical comparison identified 178 differential proteins in Neuroglobin-overexpressing SH-SY5Y cells, and the underlying mass spectrometry proteomics data are available through ProteomeXchange under PXD029012.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35128003", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Finally, the statistic comparison of the two analyzed groups within Perseus platform identified 178 differential proteins (107 up- and 71 down-regulated)."}, {"asset": "source:pmid:35128003", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "MS proteomics data are available via ProteomeXchange with the dataset identifier PXD029012."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "The statistical comparison identified 178 differential proteins in Neuroglobin-overexpressing SH-SY5Y cells, and the underlying mass spectrometry proteomics data are available through ProteomeXchange under PXD029012.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35128003"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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