Effect of PAIP1 on the metastatic potential and prognostic significance in oral squamous cell carcinoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35153296", "kind": "science-record", "revision": 5, "content_hash": "sha256:5392ae3a9b0fcb0ad3ad09eaa11f46e3dc09c93f6a2ae6523da2249e72c77700", "created_at": "2026-11-01T17:36:54Z", "updated_at": "2026-11-01T22:48:27Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Effect of PAIP1 on the metastatic potential and prognostic significance in oral squamous cell carcinoma.", "doi": "10.1038/s41368-022-00162-8", "pmid": "35153296", "authors": ["Neeti Swarup", "Kyoung-Ok Hong", "Kunal Chawla", "Su-Jung Choi", "Ji-Ae Shin", "Kyu-Young Oh", "Hye-Jung Yoon", "Jae-Il Lee", "Sung-Dae Cho", "Seong-Doo Hong"], "journal": "International journal of oral science", "year": 2022, "abstract": "Poly Adenylate Binding Protein Interacting protein 1 (PAIP1) plays a critical role in translation initiation and is associated with the several cancer types. However, its function and clinical significance have not yet been described in oral squamous cell carcinoma (OSCC) and its associated features like lymph node metastasis (LNM). Here, we used the data available from Gene Expression Omnibus (GEO), The Cancer Genome Atlas (TCGA), and Clinical Proteomic Tumor Analysis Consortium (CPTAC) to analyze PAIP1 expression in oral cancer. The publicly available data suggests that PAIP1 mRNA and protein levels were increased in OSCC. The high PAIP1 expression was more evident in samples with advanced stage, LNM, and worse pattern of invasion. Moreover, the in vitro experiments revealed that PAIP1 knockdown attenuated colony forming, the aggressiveness of OSCC cell lines, decreasing MMP9 activity and SRC phosphorylation. Importantly, we found a correlation between PAIP1 and pSRC through the analysis of the IHC scores and CPTAC data in patient samples. Our findings suggest that PAIP1 could be an independent prognostic factor in OSCC with LNM and a suitable therapeutic target to improve OSCC patient outcomes.", "claims": [{"id": "claim-method", "statement": "Multivariable correlations between the immunohistochemical staining and clinicopathological parameters of the tumors were performed via multivariable Pearson correlation, Student’s t-test, the Chi-square test, and Fisher’s exact test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35153296", "locator": "sections[24] \"Statistical analysis\" paragraph 2", "quote": "Multivariable correlations between the immunohistochemical staining and clinicopathological parameters of the tumors were performed via multivariable Pearson correlation, Student’s t-test, the Chi-square test, and Fisher’s exact test."}], "state": "complete"}, {"id": "claim-data-access", "statement": "All publicly accessed data are available on databases (TCGA, GEO, CCLE, and CPTAC) described in methodology. Analyzed datasets used during the study can be made available from the corresponding author upon reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35153296", "locator": "sections[29] \"Data availability\" paragraph 2", "quote": "All publicly accessed data are available on databases (TCGA, GEO, CCLE, and CPTAC) described in methodology. Analyzed datasets used during the study can be made available from the corresponding author upon reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "High PAIP1 expression was associated with lymph node metastasis in OSCC.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35153296", "locator": "sections[4] \"The PAIP1 expression significantly correlated with OSCC prognostic implications\" paragraph 2", "quote": "Moreover, the PAIP1 expression significantly increased in cases of nodal metastasis."}, {"asset": "source:pmid:35153296", "locator": "sections[4] \"The PAIP1 expression significantly correlated with OSCC prognostic implications\" paragraph 2", "quote": "These results data that a high PAIP1 level might be a risk factor indicating a worse OSCC prognosis."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "High PAIP1 expression was associated with lymph node metastasis in OSCC.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35153296"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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