Increased Expression of YAP Inhibited the Autophagy Level by Upregulating mTOR Signal in the Eutopic ESCs of Endometriosis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35173685", "kind": "science-record", "revision": 5, "content_hash": "sha256:45b336509705a6066ce734821bb299f106843861fda4182d7a27452ea54e37a6", "created_at": "2026-11-01T17:36:55Z", "updated_at": "2026-11-01T22:48:29Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Increased Expression of YAP Inhibited the Autophagy Level by Upregulating mTOR Signal in the Eutopic ESCs of Endometriosis.", "doi": "10.3389/fendo.2022.813165", "pmid": "35173685", "authors": ["Tianjiao Pei", "Bin Luo", "Wei Huang", "Dong Liu", "Yujing Li", "Li Xiao", "Xin Huang", "Yunwei Ouyang", "Huili Zhu"], "journal": "Frontiers in endocrinology", "year": 2022, "abstract": "We first reported that the Hippo-YAP signaling pathway plays a critical role in the pathogenesis of endometriosis (EMS). Autophagy is also related to the invasion ability of endometrial cells and is involved in the pathogenesis of EMS through multi-levels. However, the precise regulatory mechanism of YAP on autophagy in the eutopic endometrial stromal cells (ESCs) is still unclear. Primary eutopic ESCs of EMS patients (n = 12) and control patients without EMS (n = 9) were isolated and cultured to investigate the expressions of YAP and mTOR, the role of YAP in autophagy, and the effect of the YAP-autophagy signal on the decidualization of the eutopic ESCs. Endometriosis-related sequencing data (GSE51981) in the GEO database were used to find the genes significantly correlated with YAP. We found 155 genes with significant differences in the interaction with YAP in EMS from the dataset, and the autophagy pathway was significantly enriched. Following on from our previous studies of YAP knockdown, overexpression of YAP resulted in an increased expression of mTOR and decreased ratio of LC3-II/LC3-I and autophagy markers, in the eutopic ESCs; transmission electron microscope observation also showed fewer autophagosomes compared with the control cells. Furthermore, ESCs of the Rapamycin-treated group showed significant decidual-like changes with significantly increased decidual prolactin level at 72 h after in vitro decidualization. These results demonstrate that the increased YAP inhibited the level of autophagy by upregulating the mTOR signal in the eutopic ESCs of endometriosis. The YAP-autophagy signal plays an important role in the pathogenesis of endometriosis-associated infertility.", "claims": [{"id": "claim-method", "statement": "Gene differential analysis was conducted using the limma R package between EMS samples and non-EMS/normal uterus samples.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35173685", "locator": "sections[5] \"Differentially Expressed Genes\" paragraph 2", "quote": "Gene differential analysis was conducted using the limma R package between EMS samples and non-EMS/normal uterus samples."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/Supplementary Material.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35173685", "locator": "sections[23] \"Data Availability Statement\" paragraph 2", "quote": "The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/Supplementary Material."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The limma R package was used to analyze GSE51981, yielding 155 differentially expressed genes with significant differences in their interaction with YAP in endometriosis, with the autophagy pathway significantly enriched.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35173685", "locator": "sections[4] \"Data Collection\" paragraph 2", "quote": "The gene expression profile of GSE51981 was downloaded from the Gene Expression Omnibus (GEO) database (https://www.ncbi.nlm.nih.gov/geo/)."}, {"asset": "source:pmid:35173685", "locator": "sections[5] \"Differentially Expressed Genes\" paragraph 2", "quote": "Gene differential analysis was conducted using the limma R package between EMS samples and non-EMS/normal uterus samples."}, {"asset": "source:pmid:35173685", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "We found 155 genes with significant differences in the interaction with YAP in EMS from the dataset, and the autophagy pathway was significantly enriched."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The limma R package was used to analyze GSE51981, yielding 155 differentially expressed genes with significant differences in their interaction with YAP in endometriosis, with the autophagy pathway significantly enriched.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35173685"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE51981"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE51981"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:35173685", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.3389/fendo.2022.813165", "sha256": "sha256:2edc5d151c3ca7e880b1d9b4a4b4bb4f6e2bdcd0703f0f8879580799cbfdc4ca", "bytes": 47220, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.3389/fendo.2022.813165", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC8842667", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE51981", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE51981", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE51981", "version": null, "note": "The datasets presented in this study can be found in online repositories. 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Statistical Analysis A"], "order_basis": "paper_text_order:2685", "id": "assay:3"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["n efficiency of YAP was measured by qPCR and Western blotting. Statistical Analysis All statistical analys"], "order_basis": "paper_text_order:2694", "id": "assay:4"}, {"role": "analysis", "definition_id": "differential_expression", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["normalise_counts", "differential_expression_fit", "fdr_bh"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Gene differential analysis was conducted using the limma R package between EMS samples and non-EMS/normal uterus samples."], "order_basis": "consumes_earlier_output", "id": "assay:5"}]}