Regulated cell death joins in atherosclerotic plaque silent progression.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35181730", "kind": "science-record", "revision": 6, "content_hash": "sha256:7a258391469739df393eadf6461883e57c3693c5faa21df58402d0b6bbff84fa", "created_at": "2026-11-01T17:36:56Z", "updated_at": "2026-11-01T22:48:30Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Regulated cell death joins in atherosclerotic plaque silent progression.", "doi": "10.1038/s41598-022-06762-y", "pmid": "35181730", "authors": ["Elena Uyy", "Viorel I Suica", "Raluca M Boteanu", "Aurel Cerveanu-Hogas", "Luminita Ivan", "Rune Hansen", "Felicia Antohe"], "journal": "Scientific reports", "year": 2022, "abstract": "Non-apoptotic regulated cell death (ferroptosis and necroptosis) leads to the release of damage-associated molecular patterns (DAMPs), which initiate and perpetuate a non-infectious inflammatory response. We hypothesize that DAMPs and non-apoptotic regulated cell death are critical players of atherosclerotic plaque progression with inadequate response to lipid-lowering treatment. We aimed to uncover the silent mechanisms that govern the existing residual risk of cardiovascular-related mortality in experimental atherosclerosis. Proteomic and genomic approaches were applied on the ascending aorta of hyperlipidemic rabbits and controls with and without lipid-lowering treatment. The hyperlipidemic animals, which presented numerous heterogeneous atherosclerotic lesions, exhibited high concentrations of serum lipids and increased lipid peroxidation oxidative stress markers. The analyses revealed the significant upregulation of DAMPs and proteins implicated in ferroptosis and necroptosis by hyperlipidemia. Some of them did not respond to lipid-lowering treatment. Dysregulation of five proteins involved in non-apoptotic regulated cell death proteins (VDAC1, VDAC3, FTL, TF and PCBP1) and nine associated DAMPs (HSP90AA1, HSP90AB1, ANXA1, LGALS3, HSP90B1, S100A11, FN, CALR, H3-3A) was not corrected by the treatment. These proteins could play a key role in the atherosclerotic silent evolution and may possess an unexplored therapeutic potential. Mass spectrometry data are available via ProteomeXchange with identifier PXD026379.", "claims": [{"id": "claim-method", "statement": "Label-free relative quantification was performed on the precursor intensity level, with 90% of replicate features and ANOVA hypothesis test enabled.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35181730", "locator": "sections[16] \"Mass spectrometry data analysis\" paragraph 2", "quote": "Label-free relative quantification was performed on the precursor intensity level, with 90% of replicate features and ANOVA hypothesis test enabled."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The Mass spectrometry data were deposited in the PRIDE39–41 repository via ProteomeXchange with the dataset identifier PXD026379. We confirm that our study is reported in accordance with ARRIVE guidelines.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35181730", "locator": "sections[26] \"Data availability\" paragraph 2", "quote": "The Mass spectrometry data were deposited in the PRIDE39–41 repository via ProteomeXchange with the dataset identifier PXD026379. We confirm that our study is reported in accordance with ARRIVE guidelines."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Hyperlipidemia significantly upregulated DAMPs and proteins implicated in ferroptosis and necroptosis, and dysregulation of five non-apoptotic regulated cell-death proteins and nine associated DAMPs was not corrected by lipid-lowering treatment.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35181730", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The analyses revealed the significant upregulation of DAMPs and proteins implicated in ferroptosis and necroptosis by hyperlipidemia. Some of them did not respond to lipid-lowering treatment. Dysregulation of five proteins involved in non-apoptotic regulated cell death proteins (VDAC1, VDAC3, FTL, TF and PCBP1) and nine associated DAMPs (HSP90AA1, HSP90AB1, ANXA1, LGALS3, HSP90B1, S100A11, FN, CALR, H3-3A) was not corrected by the treatment."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Hyperlipidemia significantly upregulated DAMPs and proteins implicated in ferroptosis and necroptosis, and dysregulation of five non-apoptotic regulated cell-death proteins and nine associated DAMPs was not corrected by lipid-lowering treatment.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35181730"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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