Knockdown of PDX1 enhances the osteogenic differentiation of ADSCs partly via activation of the PI3K/Akt signaling pathway.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:35183219", "kind": "science-record", "revision": 6, "content_hash": "sha256:1c6efa9e8368c3fde1c8666f1f9d91aa9ac7257baed1a1c03e512424acf70425", "created_at": "2026-11-01T17:36:56Z", "updated_at": "2026-11-01T22:48:31Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Knockdown of PDX1 enhances the osteogenic differentiation of ADSCs partly via activation of the PI3K/Akt signaling pathway.", "doi": "10.1186/s13018-021-02825-4", "pmid": "35183219", "authors": ["Fan Liu", "Guang-Dong Chen", "Long-Kun Fan"], "journal": "Journal of orthopaedic surgery and research", "year": 2022, "abstract": "BACKGROUND: Osteoporosis (OP) is a systemic bone disease manifested as low bone mass, destruction of bone microstructure, increased bone fragility and fracture risk. The purpose of this study was to explore the role and mechanism of PDX1 for osteogenic differentiation of adipose derived stem cells (ADSCs).\nMETHODS: GSE37329 dataset was retrieved from NCBI Gene Expression Omnibus (GEO) database and performed bioinformatic analyses. ADSCs were incubated with normal medium, osteogenic induction medium (OIM) and OIM+si-PDX1. Then, alkaline phosphatase (ALP) staining and Alizarin Red Staining (ARS) were performed to assess the role of PDX1 for osteogenesis of ADSCs. PI3K inhibitor, LY294002 was then added to further explore the mechanism of PDX1 for osteogenic differentiation of ADSCs. Western blot assay was used to assess the osteogenic-related markers. Graphpad software was used to perform statistically analysis.\nRESULTS: A total of 285 DEGs were obtained from analysis of the dataset GSE37329, of which 145 were upregulated and 140 were downregulated genes. These differentially expressed genes mainly enriched in cell differentiation and PI3K/Akt signaling pathway. Moreover, PDX1 was decreased in osteogenic induced ADSCs. Knockdown of PDX1 significantly increased osteogenic differentiation capacity and p-PI3K and p-Akt protein levels. Administration with LY294002 could partially reversed the promotion effects of si-PDX1.\nCONCLUSION: In conclusion, knockdown of PDX1 promotes osteogenic differentiation of ADSCs through the PI3K/Akt signaling pathway.", "claims": [{"id": "claim-method", "statement": "Student’s t test or one-way analysis of variance (ANOVA) was utilized to analyze significant differences.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35183219", "locator": "sections[11] \"Statistical analysis\" paragraph 2", "quote": "Student’s t test or one-way analysis of variance (ANOVA) was utilized to analyze significant differences."}], "state": "complete"}, {"id": "claim-data-access", "statement": "We state that the data will not be shared since all the raw data are present in the figures included in the article.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:35183219", "locator": "sections[27] \"notes\" paragraph 1", "quote": "We state that the data will not be shared since all the raw data are present in the figures included in the article."}], "state": "complete"}, {"id": "claim-target-result", "statement": "In conclusion, knockdown of PDX1 promotes osteogenic differentiation of ADSCs through the PI3K/Akt signaling pathway.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:35183219", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "In conclusion, knockdown of PDX1 promotes osteogenic differentiation of ADSCs through the PI3K/Akt signaling pathway."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "In conclusion, knockdown of PDX1 promotes osteogenic differentiation of ADSCs through the PI3K/Akt signaling pathway.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:35183219"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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